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Revision as of 02:55, 17 October 2018
Overview
When considering how to develop our C. albicans detection assay into a more user friendly test, we consulted with several professionals to gain insight and conducted an Institutional Review Board (IRB) survey that provided feedback from potential users. Our process for Human Practices consisted of six basic steps.
Step 1
Doing research on the problem and learning diagnosis and treatment processes
Learning about how current tests are being used and what doctors would prefer to have was important in transforming our assay. We wanted to make sure that our diagnosis test could benefit professionals working.
Who we talked to:
Practicing OBGYN: Dr. Lisa Yang
Dr. Lisa Yang is a practicing OBGYN, who regularly sees patients, performs surgeries, and works at the hospital with labor and delivering babies. She also works with residents in training by overseeing the work they do with different tests they conduct and surgeries they perform. She answered our questions on different diagnostic tests that she conducts, which is located on the problem page.
From our talk with her we learned what her ideal detection method would encompass:
- If she is able to get information while in office and not have to send things into the lab
- If the test has a good positive predictive value and a low negative predictive value ie. accuracy
- If the test is relatively cost effective
- If her staff is able to perform the test because she either has to conduct the test herself or send it into a lab
- If the test is comparable to the gold standard test and can deliver the same results
Step 2
Developing our Paper-Based Assay
Throughout the process of building our paper-based assay, we consulted professionals who helped us change our assay to better fit the needs of healthcare professionals and patients everywhere.
Who we talked to:
Dr. Linnes
Dr. Linnes is an assistant professor in Biomedical Engineering at Purdue University who specializes in developing paper-based assays. She provided examples of paper-based assays she designed and brought to light different factors to include within our assay. Throughout the improvement of our assay, she was able to provide input and expertise in the design.
Step 3
Learn how to Relay our research to the public
Communicating our research and transporting or assay to the public is important when making our assay more globally accessible.
Who we talked to:
Dr. Cooky
Dr. Cooky is an associate professor of American Studies and Women’s, Gender, and Sexuality Studies at Purdue University. During our project she helped us in focusing in on how we can help the general public understand our research. She also brought to light that yeast infections are “a relatively minor medical issue that creates a disproportionate impact on women’s lives.”
Throughout our talk with her she brought to our attention:
- How the youth is getting educated on sexual health and what their programs entail
- Whether or not sexual health education programs are teach about infections like vulvovaginal candidiasis
- Different misconceptions that surround women’s health and the implications of what going to get treatment for infections like vulvovaginal candidiasis entail
- How different cultures view women’s health and the challenges women face in obtaining a test and getting treatment
- Make sure our test is something women could do by themselves by keeping it simple and accessible
- Consider a global reach with our test
Dr. Clase
Dr. Clase is the director of the Biotechnology Innovation and Regulatory Science Center and also a professor in Agricultural and Biological Engineering as well as Technology Leadership and Innovation. She works extensively with building programs to help secure access to medicine in Africa and developing nations. Dr. Clase helped us by answering questions about bringing our assay to the global market. From talking with her she answered our questions about international healthcare and suggested we find out how people learn about healthcare and where people go if there is no doctor or nurse.
Integrated HP:
Step 4
Changing our detection method based on the conversations with professionals
Originally we designed our assay with as many steps as necessary in order to gain the best results with the least amount of factors to account for. However, based on our talk Dr. Linnes who shed light on a method of a three step application system that users prefer. Due to this discovery, we modified our assay to fit within the three step application suggestion.
Step 5
Assess public knowledge through survey
A major part of our research was finding a way to make the assay more user-friendly than current brands. In order to do so we conducted an IRB survey that used Amazon Mechanical Turk that helped us receive feedback from a diverse demographic range.
Step 6
Public Engagement
We had the opportunity to work with Purdue University Libraries in creating an event called DIYBio and Maker, where we educated the Purdue community about synthetic biology and our project. During the event we interacted with students and shed light on the importance of synthetic biology and the implications of our research. Over the summer, we assisted in creating an escape room for high schoolers interested in synthetic biology. Before the high schoolers arrived, we went through the escape room and helped ensure that it was not too difficult and hard to understand. We also helped oversee the event to make sure everything ran smoothly.
Collaboration
A major part of our research was finding a way to make the assay more user-friendly than current brands. In order to do so we conducted an IRB survey that used Amazon Mechanical Turk that helped us receive feedback from a diverse demographic range.
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