Difference between revisions of "Team:Makerere University/Description"

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<h1>Description</h1>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<li><a href="#">igem</a></li>
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<li><a href="#">wiki tools</a></li>
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<li><a href="#">source</a></li>
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<li><a href="#">discussion</a></li>
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<li><a href="#">teams</a></li>
  
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<li><a  href="home.html">Home</a></li>
  
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<li><a class="active" href="description.html" >Project
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<h3>What should this page contain?</h3>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<h3>Inspiration</h3>
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="design.html">design</a></li>
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<li><a href="notebook.html">notebook</a></li>
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<li><a href="results.html">results</a></li></a>
  
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
 
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<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
 
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
 
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
 
 
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<li><a href="members.html" >Team
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<li><a href="members.html" >Team members</a></li>
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<li><a href="collaborations.html" >Team collaborations</a></li></a>
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<li><a href="parts.html" >Parts</a></li>
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<li><a href="safety.html" >Safety</a></li>
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<li><a href="human practices.html" >Humanpractices</a></li>
  
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<h3>Advice on writing your Project Description</h3>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements.
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<h3>References</h3>
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<h1>Project description</h1>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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<p>The project is set up to utilize the enzymatic machinery from the bacterium <b><i>Ideonella sakaiensis</i></b> as it is capable of manufacturing the enzymes of <i>PETase</i> and <i>MHETase</i> which are important in the degradation of PET. <br>
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Isolated segments of the genes for the respective enzymes are transformed into the a competent <i>E. coli</i> cell so that it also obtains the plastic degrading character as in <i>Ideonella</i>.
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<i>Ideonella sakaiensis</i> is a bacteria that naturally decomposes polyethylene terephthalate, we decide to genetically modify E. coli cells to model the plastic degradation machine by adding the Lipase and Chlorogenate Esterase genes from Ideonella sakaiensis into E. coli bacterial cells. We shall obtain the two genes encoding the enzymes used by Ideonella sakaiensis, PETase and MHETase, and insert the gene into E. coli plasmids and then put the plasmids into E. coli cells. With the engineered E.coli bacteria, the enzymes produced are able to express the plastic-degrading abilities
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<b>Goal:</b> To have a clean and safe environment free of plastic pollution.
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<b>Objectives.</b>
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<li>To engineer a bacteria capable of degrading PET plastics.</li>
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<li>To sensitize the community about the danger associated with plastic wastes.</li>
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Revision as of 11:18, 28 June 2018

Project description

Project description

The project is set up to utilize the enzymatic machinery from the bacterium Ideonella sakaiensis as it is capable of manufacturing the enzymes of PETase and MHETase which are important in the degradation of PET.
Isolated segments of the genes for the respective enzymes are transformed into the a competent E. coli cell so that it also obtains the plastic degrading character as in Ideonella.

Ideonella sakaiensis is a bacteria that naturally decomposes polyethylene terephthalate, we decide to genetically modify E. coli cells to model the plastic degradation machine by adding the Lipase and Chlorogenate Esterase genes from Ideonella sakaiensis into E. coli bacterial cells. We shall obtain the two genes encoding the enzymes used by Ideonella sakaiensis, PETase and MHETase, and insert the gene into E. coli plasmids and then put the plasmids into E. coli cells. With the engineered E.coli bacteria, the enzymes produced are able to express the plastic-degrading abilities

Goal: To have a clean and safe environment free of plastic pollution.

Objectives.

  1. To engineer a bacteria capable of degrading PET plastics.
  2. To sensitize the community about the danger associated with plastic wastes.