Difference between revisions of "Team:iTesla-SoundBio/InterLab"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="headers"><p><b><u>Summary</u>:</b> For the 2018 InterLab study, high school students at iTesla-SoundBio prepared various colonies and dilutions to test for trends absorbance, fluorescence, and concentration. For controls we took the absorbance of silica beads that are similar to cell absorbance. Then we took the absorbance of fluorescein serial dilutions to get a trend line of different absorbances as concentration of fluorescence decreases. <br> Massive thanks to the Washington iGEM InterLab team for guiding us through the protocol and lending us their plate readers. </P>
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<p>Below, is the timeline that we followed in completing the InterLab protocol.</p></div>
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<img src="https://static.igem.org/mediawiki/2018/5/55/T--iTesla-SoundBio--interlabtimeline.jpg" alt= "ludoxprotocol" style="width:80%" align= "middle">
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<div class="horizontalline">
 
</div>
 
</div>
  
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<div class="middles"><h1>Part One- LUDOX Calibration Protocol</h1></div>
  
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<div class="clear"></div>
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<div class="middles"><h2>96 Well Plate Table</h2></div>
  
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<table align="center">
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  <tr>
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    <th>  </th>
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    <th>1</th>
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    <th>2</th>
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    <th>3</th>
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    <th>4</th>
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    <th>5</th>
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    <th>6</th>
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    <th>7</th>
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    <th>8</th>
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    <th>9</th>
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    <th>10</th>
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    <th>11</th>
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    <th>12</th>
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  </tr>
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  <tr>
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    <td>A</td>
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    <td>LUDOX </td>
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    <td>ddH2O</td>
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    <td>  </td>
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    <td>  </td>
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    <td>  </td>
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    <td>  </td>
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    <td>  </td>
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    <td>  </td>
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    <td>  </td>
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    <td>  </td>
  
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<div class="column full_size">
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  </tr>
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<h1>InterLab</h1>
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  <tr>
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<h3>Bronze Medal Criterion #4</h3>
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    <td>B</td>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
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    <td>LUDOX </td>
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<br><br>
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    <td>ddH2O</td>
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For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab">InterLab study</a>, all work must be shown on this page.
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    <td>  </td>
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    <td>  </td>
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    <td> </td>
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  </tr>
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  <tr>
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    <td>C</td>
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    <td>LUDOX </td>
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    <td>ddH2O</td>
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    <td>  </td>
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  </tr>
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  <tr>
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    <td>D</td>
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    <td>LUDOX </td>
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    <td>ddH2O</td>
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    <td>  </td>
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    <td>  </td>
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    <td>  </td>
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    <td>E  </td>
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    <td> </td>
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    <td>  </td>
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      <td>F  </td>
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    <td> </td>
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    <td>  </td>
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    <td>  </td>
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    <td>G  </td>
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    <td> </td>
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    <td>  </td>
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  <tr>
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    <td>H  </td>
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    <td> </td>
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<div class="middles">
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<p>Here are the results for the Absorbance 600 of the LUDOX protcol</p>
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<h2> OD600 Reference Point Data </h2>
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<img src="https://static.igem.org/mediawiki/2018/8/8b/T--iTesla-SoundBio--LUDOX_absorbance_readings.jpeg.JPG" alt= "ludoxprotocol">
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<h1> Part Two- Microsphere Protocol</h1>
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<img src="https://static.igem.org/mediawiki/2018/f/f6/T--iTesla-SoundBio--microspherestandardcurvelinear.jpg" alt= "microspherecurve", style="width:40%;">
  
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<img src="https://static.igem.org/mediawiki/2018/6/64/T--iTesla-SoundBio--microspherestandardcurvelog.jpg" alt= "microsphere log curve", style="width:40%;">
  
  
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<div class="middles"><h1> Part Three- Fluorescein Protocol</h1><div>
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<img src="https://static.igem.org/mediawiki/2018/4/44/T--iTesla-SoundBio--fluoresceinlinear.jpg" alt= "fluoresciencurve", style="width:40%;">
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<img src="https://static.igem.org/mediawiki/2018/5/51/T--iTesla-SoundBio--fluorescein.jpg" alt= "microsphere log curve", style="width:40%;">
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<br>
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</p>
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</div>
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<img src="https://static.igem.org/mediawiki/2018/f/fe/T--iTesla-SoundBio--interlabjoy.jpg" alt= "interlab joy", style="width:80%;">
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<p> Our experience with Interlab in one word would be rough. Our lab did not have a -80 degree freezer at that time so we had to store our competent cells at -20 degrees. One issue we had was when the InterLab leads were not present at the wetlab session to transform the E. coli cells with the devices, the members that were present used all the DNA that was provided. So when we had to re-do our InterLab protocols, we had to think of work arounds for the protocols. Additionally, we had to use the plate reader at Washington iGEM lab meaning that we had to schedule the wetlab days taking into account the waiting steps according to the schedule of the Washington iGEM team.
  
 +
Some possible sources of error or limitations were that we did not have black 96 well plates with a clear bottom. Instead, we used a 96 well plate that was clear throughout.
  
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Latest revision as of 05:31, 17 October 2018

Safety Protocols
InterLab
Team Members Attributions
Human Practices Collaborations
Project Description Design Results Biobrick Parts Lab Notebook
iT-SB 2018 iT-SB 2017

Summary: For the 2018 InterLab study, high school students at iTesla-SoundBio prepared various colonies and dilutions to test for trends absorbance, fluorescence, and concentration. For controls we took the absorbance of silica beads that are similar to cell absorbance. Then we took the absorbance of fluorescein serial dilutions to get a trend line of different absorbances as concentration of fluorescence decreases.
Massive thanks to the Washington iGEM InterLab team for guiding us through the protocol and lending us their plate readers.

Below, is the timeline that we followed in completing the InterLab protocol.

ludoxprotocol

Part One- LUDOX Calibration Protocol

96 Well Plate Table

1 2 3 4 5 6 7 8 9 10 11 12
A LUDOX ddH2O
B LUDOX ddH2O
C LUDOX ddH2O
D LUDOX ddH2O
E
F
G
H

Here are the results for the Absorbance 600 of the LUDOX protcol

OD600 Reference Point Data

ludoxprotocol

Part Two- Microsphere Protocol

microspherecurve microsphere log curve

Part Three- Fluorescein Protocol

fluoresciencurve microsphere log curve


interlab joy

Our experience with Interlab in one word would be rough. Our lab did not have a -80 degree freezer at that time so we had to store our competent cells at -20 degrees. One issue we had was when the InterLab leads were not present at the wetlab session to transform the E. coli cells with the devices, the members that were present used all the DNA that was provided. So when we had to re-do our InterLab protocols, we had to think of work arounds for the protocols. Additionally, we had to use the plate reader at Washington iGEM lab meaning that we had to schedule the wetlab days taking into account the waiting steps according to the schedule of the Washington iGEM team. Some possible sources of error or limitations were that we did not have black 96 well plates with a clear bottom. Instead, we used a 96 well plate that was clear throughout.