Difference between revisions of "Team:ZJU-China/Attributions"

Welcome! This year, we designed 23 parts, and all of them have been submitted to the iGEM Registry following the Biobricks assembly standard as described by RFC10. See more detailed information on our part pages !

This year ZJU-China consists of 14 members, who can be separated into 2 groups, including the wet-lab group and the dry-lab group. Wet-lab group members have the background in life science and medical science while the dry-lab group members mainly major in engineering and computer science. This year, ZJU-China focused on developing an in-vitro diagnostic device whose idea was first brought out after rounds of brainstorming. After identifying the direction of our project, we always worked together, designing and optimizing our products rounds by rounds accompanied with a continuous process of Human Practice.

Unless otherwise stated, all the experiments, constructs, logos and results documented on our wiki were performed, composed and collected by ourselves.

Lab and modelling support

Sincere thanks to whoever helped us!

Interlab support

Associate Professor Xianyin Zhang provided guidance and permissions on FCM.

Lab support

Associate Professor Fan Yang, Professor Min Wu, Professor Ming Chen helped us maintain our laboratory work!

Professor Neel Joshi, Professor Urartu Seker, Professor Chao Zhong gave us valuable advice on our experiments!

Professor Chao Zhong provided us with the CsgA Knockout Bacteria Strain From E.Coli MG1655.

Professor Bin Su provided guidance and permissions on electrochemical workstation.

Director of Clinical Laboratory in Run Run Shaw Hospital, Jun Zhang, Director of General Surgery Department in Run Run Shaw Hospital, Yifan Wang, Doctor Hong Jin, Doctor Jingjuan Song, Doctor Wei Jin helped us apply our diagnostic device in Run Run Shaw Hospital.

Storage

The chemical and biological reagents are stored in the proper place and suitable temperature. Hazardous or corrosive chemicals are stored separately.

Fig.2 Storage for chemical and biological reagents

Waste

Waste should be classified and disposed of separately.

Safety Apparatus

Our iGEM lab is equipped with safety apparatus such as fire extinguishers, emergency showers, eyewash stations, and first aid kits in case of dire situations.

Fig.3 The fire extinguisher

Fig.4 The fire blanket

Fig.5 The emergency shower

Fig.6 The eyewash equipment

Fig.7 The first-aid case

References

[1] Michelle M. Barnhart Matthew R. Chapman. Curli Biogenesis and Function. Annual Review of Microbiology(2006):131-147

[2] CDC.Recommendations for Prevention of HIV Transmission in Health-Care Settings.MMWR,1987;36

[3] Z Botyanszki, PKR Tay, PQ Nguyen, MG Nussbaumer, NS Joshi.Engineered catalytic biofilms: Site-specific enzyme immobilization onto E. coli curli nanofibers. Biotechnology & Bioengineering, 2015, 112 (10) :2016-2024

Our Sponsors

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 			<div class="psg" >
-				<span class="psg_ttl">Project Safety</span>
+				<span class="psg_ttl">Lab and modelling support</span>
-				<span class="psg_subtitle">Biofilm Scaffold</span>
+				<p>Sincere  thanks to whoever helped us!</p>
-				<p>Our biofilm scaffold consists of curli which is the protein found on the surface of E.Coli. Curli may facilitate bacteria’s invasion into host cells and activate corresponding cytokines and inflammatory mediators in plasma. But the system we wish to produce will be ultimately cell-free and no living organisms will be included. Besides, curli will be immobilized and covered by Nafion on the electrode, so contact with curli will not happen. Therefore, biofilm scaffold is relatively safe.</p>
-				<span class="psg_subtitle">Blood Samples</span>
-				<p>We have gotten approval from our university authority and collected some blood samples from SIR RUN RUN SHAW HOSPITAL, which is classified as a Grade 3 Class A hospital by Chinese government. The procedure of blood sampling was done by medical personnel using ideal blood collection equipment. Besides, our experiment was conducted under professional doctors' supervision and waste was collected by the specialties in the hospital. (It is also an essential part of <a href="#">Integrated Human Practice.</a>) </br></p>
-<p></br>Our project meets ethical requirements. Blood samples we got from hospital are all remaining samples after satisfying patients’ pathological diagnosis need. We keep secret of patients’ privacy information. We conduct experiments with clinical material following our country's laws and our university's rules.</br> </p>
-<p></br>The main issue for work with blood samples is the potential for infection. Clinical samples may be contaminated with pathogens. The high risk, well known viral agents such as Human Immunodeficiency Virus (HIV), Hepatitis B virus (HBV) and Hepatitis C Virus (HCV) are not the only agents that may be present in blood. Other viruses such as HTLV1 and B19 as well as various bacterial agents may also be present. Regarding the likely incidence of a pathogen in blood samples, several factors should be considered. These include known medical history of a patient or donor, whether the samples are from individuals showing clinical symptoms of infectious disease, the incidence of the various pathogens that are endemic in the local population or donor group and the type of sample. Fortunately, SIR RUN RUN SHAW HOSPITAL offered us the physiological indices of blood samples and other relevant information. So, the samples we used are from formal approach and relatively less dangerous. To minimize the risks，we have made Standard Operating Procedure (SOP) which is strictly followed in our iGEM lab.</p>
-				<span class="psg_subtitle">Standard Operating Procedure (SOP)</span>
-				<p>As insurance, Blood samples from all patients should be considered infective. The following precautions followed in our lab are also recommended for all health-care workers in clinical laboratories.</p>
-				<p style="padding-left:.5em; margin-top:.5em;">1. All the experiments where blood is involved must be conducted in a separate room within the laboratory.</p>
-<p style="padding-left:.5em; margin-top:.5em;">2. All specimens of blood should be put in a well- constructed container with a secure lid to prevent leaking. Care should be taken when dealing with each specimen to avoid contaminating the outside of the container and of the laboratory form accompanying the specimen.</p>
-<p style="padding-left:.5em; margin-top:.5em;">3. Lab surface including correlated equipment is decontaminated with chemical disinfectant prior and after disposal.</p>
-<p style="padding-left:.5em; margin-top:.5em;">4. Every piece of material that has been in direct contact with blood should be disposed of correctly via the clinical waste route when work activities are completed.</p>
-<p style="padding-left:.5em; margin-top:.5em;">5. All persons processing blood specimens should wear gloves. Gloves should be changed, and hands washed after completion of specimen processing.</p>
-<p style="padding-left:.5em; margin-top:.5em;">6. Use of sharp objects (e.g. needles, syringes, scissors) should be limited for fear of injuries and cross infection.</p>
-<p style="padding-left:.5em; margin-top:.5em;">7. All persons should wash their hands after completing blood specimens’ processing.</p>
-				<span class="psg_ttl">General Safety</span>
+			<span class="psg_ttl">Interlab support</span>
-<p>Working with living modified organisms is always a risk. All standard precautions must be taken. Before starting to work in the lab, every member of our team was asked to attend laboratory safety lesson and take an biosafety examination.</p>
+<p>Associate Professor Xianyin Zhang provided guidance and permissions on FCM.</p>
-				<span class="psg_ttl psg_subtitle">Wearing</span>
+				<span class="psg_ttl">Lab support</span>
-<p>Everyone wears protective lab coats, enclosed leather shoes, gloves and safety glasses before laboratory activities.</br></p>
+<p>Associate Professor Fan Yang, Professor Min Wu, Professor Ming Chen helped us maintain  our laboratory work! </p></br>
-<img src="https://static.igem.org/mediawiki/2018/0/01/T--ZJU-China--Safety1.jpg" style="width:500px">
+<p>Professor Neel Joshi, Professor Urartu Seker, Professor Chao Zhong gave us valuable advice on our experiments! </p></br>
-				<h5 style="color: black;">Fig.1 Working with the Clean Bench</h5>
+<p>Professor Chao Zhong provided us with the CsgA Knockout Bacteria Strain From E.Coli MG1655. </p></br>
+<p>Professor Bin Su provided guidance and permissions on electrochemical workstation. </p></br>
+<p>Director of Clinical Laboratory in Run Run Shaw Hospital, Jun Zhang, Director of General Surgery Department in Run Run Shaw Hospital, Yifan Wang, Doctor Hong Jin, Doctor Jingjuan Song, Doctor Wei Jin helped us apply our diagnostic  device in Run Run Shaw Hospital. </p></br>