Difference between revisions of "Team:Jiangnan/Results"

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{{Jiangnan}}
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.JTrow{
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<h1>Results</h1>
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<p>Here you can describe the results of your project and your future plans. </p>
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</div>
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<h3>What should this page contain?</h3>
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.JTp{
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<ul>
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    display: block;
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<li> Clearly and objectively describe the results of your work.</li>
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    margin-block-start: 0.2em;
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<li> Future plans for the project. </li>
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    margin-block-end: 0.2em;
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<li> Considerations for replicating the experiments. </li>
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</ul>
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.Jnavtitle{float: right;width: 10%;text-align: center;padding: 1em 0;}
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.Jnavdrag>ul{position: absolute;top: 0;width: 100%;border-radius: 5px;background-color: white;transition: all .4s ease-in-out;opacity: 0;}
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</script>
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<div class="Jnav">
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<div class="Jnavtitle">
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<a href="https://2018.igem.org/Team:Jiangnan/Safety">Safety</a>
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</div>
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<div class="Jnavtitle">
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<a href="https://2018.igem.org/Team:Jiangnan/Hardware">Hardware</a>
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</div>
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<div class="Jnavtitle" onmouseover="Jnavshow(this)" onmouseleave="Jnavhide(this)">
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<a href="https://2018.igem.org/Team:Jiangnan/Team">Team</a>
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<div class="Jnavdrag">
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<ul>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Team">Team Members</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Attributions">Attribution</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Collaborations">Collaboration</a></li>
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</ul>
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</div>
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</div>
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<div class="Jnavtitle" onmouseover="Jnavshow(this)" onmouseleave="Jnavhide(this)">
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<a href="https://2018.igem.org/Team:Jiangnan/Human_Practices">Human Practice</a>
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<div class="Jnavdrag">
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<ul>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Human_Practices">Overview</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Silver">Silver</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Entrepreneurship">Gold</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Public_Engagement">Pulic Engagement</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Entrepreneurship">Entrepreneurship</a></li>
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</ul>
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</div>
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</div>
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<div class="Jnavtitle">
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<a href="https://2018.igem.org/Team:Jiangnan/Model">Model</a>
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</div>
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<div class="Jnavtitle" onmouseover="Jnavshow(this)" onmouseleave="Jnavhide(this)">
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<a href="https://2018.igem.org/Team:Jiangnan/Notebook">Notebook</a>
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<div class="Jnavdrag">
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<ul>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Notebook">Lab Book</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Protocol">Protocol</a></li>
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</ul>
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</div>
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</div>
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<div class="Jnavtitle" onmouseover="Jnavshow(this)" onmouseleave="Jnavhide(this)">
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<a href="https://2018.igem.org/Team:Jiangnan">Project</a>
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<div class="Jnavdrag">
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<ul>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Background">Background</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Design">Design</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Demonstrate">Demonstration</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Results">Result</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Parts">Part</a></li>
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</ul>
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</div>
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</div>
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<div class="navlogo" style="float: left;width: 20%;text-align: center;">
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<a href="https://2018.igem.org/Team:Jiangnan"><img src="https://static.igem.org/mediawiki/2018/d/d7/T--Jiangnan--igemJN_logo.png" style="width: 3em;"></a>
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</div>
 
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<h3>Describe what your results mean </h3>
 
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<ul>
 
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
 
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
 
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
 
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<img src="https://static.igem.org/mediawiki/2018/7/74/T--Jiangnan--result_top.png" width="100%">
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<h3> Project Achievements </h3>
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<div class="col s10 offset-s1">
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<h5>Broad Spectrum</h5>
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<p>Both biobricks were successfully constructed, and the Nectin 4 biobrick has been transfected into MDBK cells, and functionally expressed on the surface of MDBK cells as validated by its enabled sensitivity to CDV infection.</p>
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<ul>
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</div>
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<li>A list of linked bullet points of the successful results during your project</li>
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<div class="col s10 offset-s1">
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<h5>Suspension Cultivation</h5>
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</ul>
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<p>
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·We identified PABPC1 as the candidate gene responsible for cell suspension through analyzing the RNA-Seq results of adherent and suspended cells from the same line and origin.<br>
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·We validated that suspended cells have low PABPC1 expression by qPCR.<br>
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·We validated that PABPC1 under expression can make cells feasible for suspension cultivation.</p>
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</div>
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<div class="col s10 offset-s1">
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<h5>High Titer</h5>
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<p>
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IRF7 expression was sufficiently suppressed.<br>
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Virus titer was increased over 2 folds once suppressing IRF7 expression.<br>
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Two mins cold atmospheric plasma treatment can further increase virus titer 2.5 folds.</p>
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</div>
 
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</div>
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<div class="highlight decoration_A_full">
 
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<h3>Inspiration</h3>
 
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<p>See how other teams presented their results.</p>
 
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<ul>
 
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
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</ul>
 
 
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Revision as of 06:18, 17 October 2018

Safety
Hardware
Team
Human Practice
Model
Notebook
Project
Broad Spectrum

Both biobricks were successfully constructed, and the Nectin 4 biobrick has been transfected into MDBK cells, and functionally expressed on the surface of MDBK cells as validated by its enabled sensitivity to CDV infection.

Suspension Cultivation

·We identified PABPC1 as the candidate gene responsible for cell suspension through analyzing the RNA-Seq results of adherent and suspended cells from the same line and origin.
·We validated that suspended cells have low PABPC1 expression by qPCR.
·We validated that PABPC1 under expression can make cells feasible for suspension cultivation.

High Titer

IRF7 expression was sufficiently suppressed.
Virus titer was increased over 2 folds once suppressing IRF7 expression.
Two mins cold atmospheric plasma treatment can further increase virus titer 2.5 folds.