Difference between revisions of "Team:ECUST/Protocol"
| Line 29: | Line 29: | ||
<tr><td>DNA template</td><td>1</td></tr> | <tr><td>DNA template</td><td>1</td></tr> | ||
<tr><td>EasyPfu DNA Polymerase</td><td>1</td></tr> | <tr><td>EasyPfu DNA Polymerase</td><td>1</td></tr> | ||
| + | </table> | ||
| + | <p>Thermocycling</p> | ||
| + | <p>The PCRmachine should be set to run the following steps</p> | ||
| + | <table border="1"> | ||
| + | <tr><th>Pre-denaturation</th><th>94℃</th><th colspan="2">5min</th></tr> | ||
| + | <tr><td>transsexual</td><td>94℃</td><td>30s</td><td rowspan="3">30circles</td></tr> | ||
| + | <tr><td>annealing</td><td>Tm-5℃</td><td>30s</td></tr> | ||
| + | <tr><td>extend</td><td>72℃</td><td>0.5kb/min</td></tr> | ||
| + | <tr><td>extend</td><td>72℃</td><td colspan="2">5min</td></tr> | ||
</table> | </table> | ||
</div> | </div> | ||
Revision as of 06:20, 17 October 2018