Difference between revisions of "Team:IISER-Bhopal-India"
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<p align="justify" style="font-family:'Roboto', sans-serif; font-size:16px; font-weight:300; line-height:1.6em; color:#656565;">Methane is a Greenhouse gas associated with Global Warming,and green methods are desired for its real-time monitoring. | <p align="justify" style="font-family:'Roboto', sans-serif; font-size:16px; font-weight:300; line-height:1.6em; color:#656565;">Methane is a Greenhouse gas associated with Global Warming,and green methods are desired for its real-time monitoring. | ||
Thus, we have developed the prototype of a robust field-applicable methane biosensor, | Thus, we have developed the prototype of a robust field-applicable methane biosensor, | ||
| − | MethNote. We found an enzyme-complex methane monooxygenase(MMO) from Methylococcus capsulatus, | + | MethNote. We found an enzyme-complex methane monooxygenase(MMO) from <i>Methylococcus capsulatus</i>, |
a methanotrophic bacterium, that converts methane to methanol. We expressed soluble-MMO in the methylotrophic yeast, | a methanotrophic bacterium, that converts methane to methanol. We expressed soluble-MMO in the methylotrophic yeast, | ||
| − | Pichia pastoris, which harbors a plasmid expressing the reporter gene under a methanol inducible promoter AOX. | + | <i>Pichia pastoris</i>, which harbors a plasmid expressing the reporter gene under a methanol inducible promoter AOX. |
Thus, linking methane uptake to a reporter gene expression generates the proposed methane biosensor. The inclusion of | Thus, linking methane uptake to a reporter gene expression generates the proposed methane biosensor. The inclusion of | ||
sMMO pathway was also checked by metabolic modeling. The constructed part will be a useful contribution to the iGEM repository. | sMMO pathway was also checked by metabolic modeling. The constructed part will be a useful contribution to the iGEM repository. | ||
| − | A commercial design of MethNote will find widespread applications in environmental monitoring of methane. In future studies, | + | A commercial design of <b>MethNote</b> will find widespread applications in environmental monitoring of methane. In future studies, |
| − | we also anticipate an additional application of Mut- strain of P. pastoris expressing sMMO in biofuel production through | + | we also anticipate an additional application of Mut- strain of <i>P. pastoris</i> expressing sMMO in biofuel production through |
methanol sequestration.</p> | methanol sequestration.</p> | ||
</div> | </div> | ||
Revision as of 07:17, 17 October 2018
Overview
Methane is a Greenhouse gas associated with Global Warming,and green methods are desired for its real-time monitoring. Thus, we have developed the prototype of a robust field-applicable methane biosensor, MethNote. We found an enzyme-complex methane monooxygenase(MMO) from Methylococcus capsulatus, a methanotrophic bacterium, that converts methane to methanol. We expressed soluble-MMO in the methylotrophic yeast, Pichia pastoris, which harbors a plasmid expressing the reporter gene under a methanol inducible promoter AOX. Thus, linking methane uptake to a reporter gene expression generates the proposed methane biosensor. The inclusion of sMMO pathway was also checked by metabolic modeling. The constructed part will be a useful contribution to the iGEM repository. A commercial design of MethNote will find widespread applications in environmental monitoring of methane. In future studies, we also anticipate an additional application of Mut- strain of P. pastoris expressing sMMO in biofuel production through methanol sequestration.