Difference between revisions of "Team:CUNY Kingsborough/Design"

Line 1: Line 1:
 
{{:Team:CUNY_Kingsborough/Header}}
 
{{:Team:CUNY_Kingsborough/Header}}
 +
{{:Template:CUNY_Kingsborough/CSS}}
  
 
<html>
 
<html>
−
<meta charset="UTF-8">
 
−
<meta name="viewport" content="width=device-width, initial-scale=1">
 
−
<link rel="stylesheet" href="https://www.w3schools.com/w3css/4/w3.css">
 
−
<link rel="stylesheet" href="https://fonts.googleapis.com/css?family=Raleway">
 
−
 
−
<div class="column full_size">
 
−
<h1>Design</h1>
 
−
<p>
 
−
Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
 
−
</p>
 
−
 
−
<p>
 
−
This page is different to the "Applied Design Award" page. Please see the <a href="https://2018.igem.org/Team:CUNY_Kingsborough/Applied_Design">Applied Design</a> page for more information on how to compete for that award.
 
−
</p>
 
−
 
−
</div>
 
−
 
−
 
−
 
−
<div class="column two_thirds_size">
 
−
<h3>What should this page contain?</h3>
 
−
<ul>
 
−
<li>Explanation of the engineering principles your team used in your design</li>
 
−
<li>Discussion of the design iterations your team went through</li>
 
−
<li>Experimental plan to test your designs</li>
 
−
</ul>
 
−
 
−
</div>
 
−
 
−
<div class="column third_size">
 
−
<div class="highlight decoration_A_full">
 
−
<h3>Inspiration</h3>
 
−
<ul>
 
−
<li><a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">2016 MIT</a></li>
 
−
<li><a href="https://2016.igem.org/Team:BostonU/Proof">2016 BostonU</a></li>
 
−
<li><a href="https://2016.igem.org/Team:NCTU_Formosa/Design">2016 NCTU Formosa</a></li>
 
−
</ul>
 
−
</div>
 
−
</div>
 
  
 +
<body>
 +
<h1 class="title-padding">Design</h1>
  
 +
<h2 class="low-rise-padding">Kill Switch for Engineered Bacteria</h2>
 +
<p class="no-rise-padding">In 2016, <a id="bodyLink" href="https://2016.igem.org/Team:Kingsborough_NY">we engineered E.coli to break down nitrogenous waste in Jamaica Bay</a>. In 2017, <a id="bodyLink" href="https://2017.igem.org/Team:Kingsborough_NY">we designed a light-activated kill switch to prevent our bacteria from surviving in saltwater outside of the waste treatment plant</a>. Within this design we had two versions - one with MazF which causes high cell death, and one with LacZ which allowed us to collect data on expression levels without having to worry about high toxicity.</p>
  
 +
<h2 class="low-rise-padding">Design of Light-Activated Kill Switch</h2>
 +
<h3 class="low-rise-padding">K2268005</h3>
 +
<center><img src="https://static.igem.org/mediawiki/2018/5/5b/T--CUNY_Kingsborough--pdawnlacz.jpeg" width=35%"></center>
 +
<h3 class="low-rise-padding">K2268006</h3>
 +
<center><img src="https://static.igem.org/mediawiki/2018/9/9f/T--CUNY_Kingsborough--pdawnmazfssra.jpeg" width="35%"></center>
 +
</body>
 
</html>
 
</html>

Revision as of 09:31, 17 October 2018

Background Design Experiments Results
Overview Modeling 101 Light Operon Turing Patterns CRISPR-Cas13a
Human Practices Public Engagement
Team Members Collaborations Attributions

Design

Kill Switch for Engineered Bacteria

In 2016, we engineered E.coli to break down nitrogenous waste in Jamaica Bay. In 2017, we designed a light-activated kill switch to prevent our bacteria from surviving in saltwater outside of the waste treatment plant. Within this design we had two versions - one with MazF which causes high cell death, and one with LacZ which allowed us to collect data on expression levels without having to worry about high toxicity.

Design of Light-Activated Kill Switch

K2268005

K2268006