Difference between revisions of "Team:NAU-CHINA/testh"

 
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Mosfet
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Overview
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1． What is Mosfet?
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We developed iGEMCloud to solve some of iGEM's current problems, with its immediacy, openness and interactivity. At present, all the information about iGEM comes from many websites. They are scattered, cluttered and unfriendly to personal user.
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The metal-oxide-semiconductor field-effect transistor (MOSFET, MOS-FET, or MOS FET) is a type of field-effect transistor (FET). It has an insulated gate, whose voltage determines the conductivity of the device. This capacity to change conductivity by varying the amount of applied voltage can be used for amplifying or switching electronic signals. In an enhancement MOSFET mode, voltage applied to the gate terminal increases the conductivity of the device. In depletion mode transistors, voltage applied at the gate reduces the conductivity.
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The MOSFET is by far the most common transistor in digital circuits,. Millions of transistors may be included in a memory chip or microprocessor.
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So we have created this application(So far, it is android based, and we hope to launch the iOS version in the future) , which is hoped to be a information gathering platform that receives the latest information of iGEM, a micro-blog that allows all users to participate in discussions in group or individual, and a community that built for iGEMers all around the world.
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2． Why are our cells also called Mosfet?
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Inspired by electronic Mosfet, we designed our project, Monitoring and Operating System Founded on Engineered T cells,which is very similar to Mosfet in many ways. Our Mosfet can be divided into two systems, signal detection system and signal response system.
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Instructions
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1) Signal detection
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1.Register an account with your email address, enter the verify code, and use this address to log in.
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We use synthetic Notch (synNotch) as our signal detection receptor of the engineered T cell. When the recognition domain binds to its target antigen, it will release the effector domain (ED) and the ED is TEV protease.  
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2) Signal processing
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2.In the application, you can receive official news, notifications, etc. And so that you can get the latest news of iGEM events in a timely, convenient and accurate way.
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We want to convert the extracellular analog signal into an intracellular digital signal . We use Tet operator (TetO) to achieve this goal. This part contains TetO, TEV protease from the ED of synNotch and Tet repressor (TetR) from 2017 Oxford University iGEM project. We can initially implement the filtering function by using these elements but also has leakage because of TetO and TetR.
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To solve the leakage problem, we introduced a recombinase into the system. The recombinase is capable of flipping the 5' end to the 3' end of the target sequence containing the specific sites, thereby realizing the zero-one switch precisely.
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3.Users can write blog posts in the discussion forum for all users to browse. The blog post is in the form of pictures + text. The blog posts are classified into different sections, such as experiment, seek help, etc. And so that you can share your ideas with all iGEM participants.
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3) Signal output
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Finally, we achieved equal outputs by using promoters which have different  strength and recombinases which have different effects . Because the promoter was located at downstream of TetO and upstream of recombinase, the function is self-evident (if you want to see more, you can click here).
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Excellent blog posts will be tagged so that they are discovered by users. At the same time, users who see the blog posts written by you can comment or reply to you. The content of the reply is in the form of image + text. When you find a nice blog post, click on the star symbol in the upper right to bookmark it for later reading.
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4) Reset
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When the external signal disappears or falls below the threshold, our mosfet has a reset function, which is to restore the initial state. This function is achieved by a protein called recombination directionality factor (RDF).
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4.If you want to remind specific users to view the content you have posted, you can remind the user to view this blog post or comment by "@" + "user nickname".
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5) The relationship between electronic Mosfet and our Mosfet
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From another perspective, the original Mosfet loaded into the circuit can do the same thing as our Mosfet: 1) constantly detect the voltage signal, 2) to process voltage signal(change the resistance),3) output the corresponding current, and 4) immediately restore the original state after the external changed voltage is restored. But the principles to achieve these are completely different,so the extension must be combined with the actual situation.
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5.You can search for blog posts in one or more keywords in the app, and sort the search results by time, number of view , and collection. You can also search for users or teams.
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6.You can follow the teams or users that you think are worthy of attention so that you can view their published content more easily in the future.
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7.Each user can create or join a team, become a member, and use their team space. The team creator is the captain who can edit the team information in the team space for others to join and show the team research direction. Only members of the team can post in the team space, but everyone can view it. Also, the captain can add albums in the team space,where members of the team can post photos.
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8.This app has built-in gadgets that may be needed for the lab and more other features will be added in the future...
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What it could be
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An information gathering platform:
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We will gather information from other website to release, and we hope to build contact with some organization to get instant and accurate information. The user of this app can share their information in it. If it is valuable and reliable, we will push it to everyone.
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A platform for interaction between teams:
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Through the form of the team space, each team can easily interact with other teams. This will strengthen the connection between iGEM teams and cause countless positive possibilities.
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An iGEM community:
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Each iGEMer could leave a footprint here and show themselves here. Everyone here is part of the iGEM.
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The App we conceived can provide comprehensive information on the release of events, each team to register their own team home page, various activities related to iGEM, question answer and experience sharing , topic discussion, experimental supplies, and more.

Latest revision as of 10:05, 17 October 2018

Overview We developed iGEMCloud to solve some of iGEM's current problems, with its immediacy, openness and interactivity. At present, all the information about iGEM comes from many websites. They are scattered, cluttered and unfriendly to personal user.

So we have created this application(So far, it is android based, and we hope to launch the iOS version in the future) , which is hoped to be a information gathering platform that receives the latest information of iGEM, a micro-blog that allows all users to participate in discussions in group or individual, and a community that built for iGEMers all around the world.

Instructions 1.Register an account with your email address, enter the verify code, and use this address to log in.

2.In the application, you can receive official news, notifications, etc. And so that you can get the latest news of iGEM events in a timely, convenient and accurate way.

3.Users can write blog posts in the discussion forum for all users to browse. The blog post is in the form of pictures + text. The blog posts are classified into different sections, such as experiment, seek help, etc. And so that you can share your ideas with all iGEM participants.

Excellent blog posts will be tagged so that they are discovered by users. At the same time, users who see the blog posts written by you can comment or reply to you. The content of the reply is in the form of image + text. When you find a nice blog post, click on the star symbol in the upper right to bookmark it for later reading.

4.If you want to remind specific users to view the content you have posted, you can remind the user to view this blog post or comment by "@" + "user nickname".

5.You can search for blog posts in one or more keywords in the app, and sort the search results by time, number of view , and collection. You can also search for users or teams.

6.You can follow the teams or users that you think are worthy of attention so that you can view their published content more easily in the future.

7.Each user can create or join a team, become a member, and use their team space. The team creator is the captain who can edit the team information in the team space for others to join and show the team research direction. Only members of the team can post in the team space, but everyone can view it. Also, the captain can add albums in the team space,where members of the team can post photos.

8.This app has built-in gadgets that may be needed for the lab and more other features will be added in the future...

What it could be An information gathering platform: We will gather information from other website to release, and we hope to build contact with some organization to get instant and accurate information. The user of this app can share their information in it. If it is valuable and reliable, we will push it to everyone.

A platform for interaction between teams: Through the form of the team space, each team can easily interact with other teams. This will strengthen the connection between iGEM teams and cause countless positive possibilities.

An iGEM community: Each iGEMer could leave a footprint here and show themselves here. Everyone here is part of the iGEM.

The App we conceived can provide comprehensive information on the release of events, each team to register their own team home page, various activities related to iGEM, question answer and experience sharing , topic discussion, experimental supplies, and more.