Difference between revisions of "Team:NAU-CHINA/testh"

 
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Overview
 
Overview
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Due to the complex circuit design of our subject, the numerous combination of promoters and gene elements lead to the effect on the whole system in case of the malfunction of any parts, making it difficult for us to locate the malfunction. Therefore, we decided to adopt the method of debugging the program usually employed by computer programmers.
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We developed iGEMCloud to solve some of iGEM's current problems, with its immediacy, openness and interactivity. At present, all the information about iGEM comes from many websites. They are scattered, cluttered and unfriendly to personal user.
  
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1)Verify the function of each part.
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So we have created this application(So far, it is android based, and we hope to launch the iOS version in the future) , which is hoped to be a information gathering platform that receives the latest information of iGEM, a micro-blog that allows all users to participate in discussions in group or individual, and a community that built for iGEMers all around the world.
  
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2)Combine the parts into two large modules of upstream and downstream circuits to verify the function.
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Instructions
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1.Register an account with your email address, enter the verify code, and use this address to log in.
  
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3)Assemble the upstream and downstream circuits to verify the function of the whole system.
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2.In the application, you can receive official news, notifications, etc. And so that you can get the latest news of iGEM events in a timely, convenient and accurate way.
  
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However, due to time constraints, we cannot complete such detailed and complete functional verification of various combination designs in just a few months. We have completed the functional verification for most parts and some of the upstream and downstream circuits, but time does not allow us to combine the upstream and downstream circuits for final functional verification. This is undoubtedly regretful, but we have provided concrete ideas for future experiments to help us complete the improvement of the subject. We also put these future experiments on our Wiki.
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3.Users can write blog posts in the discussion forum for all users to browse. The blog post is in the form of pictures + text. The blog posts are classified into different sections, such as experiment, seek help, etc. And so that you can share your ideas with all iGEM participants.
  
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Demonstrate
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Excellent blog posts will be tagged so that they are discovered by users. At the same time, users who see the blog posts written by you can comment or reply to you. The content of the reply is in the form of image + text. When you find a nice blog post, click on the star symbol in the upper right to bookmark it for later reading.
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Upstream circuit
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The upstream circuit mainly designs a signal path to enable cells to receive specific external signals and activate the downstream core circuit to realize the threshold function. Therefore, the upstream circuit can be replaced considering different situations. Here, we provide an upstream circuit design as a reference and other researchers can design their own upstream path to their own needs.
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Customizing the signal path of cells in response to external signals
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4.If you want to remind specific users to view the content you have posted, you can remind the user to view this blog post or comment by "@" + "user nickname".
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There is a wide type of extracellular signals. Cells receive extracellular signals and respond to the signal molecules accordingly. We hope to customize a kind of receptor so that it can recognize the signal molecules and regulate downstream gene expression [1]. We choose synNotch as an ideal receptor.
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Similar to some signal molecules, take Epidermal Growth Factor Receptor in our realistic system as example, the GFP is also protein but more stable and has no impact on the system. As for visibility and operability, cell surface-expressed GFP as a model of extracellular signal molecule is a better choice. Therefore, we want to replace the excellular domain of synNotch with Lag16, a kind of antigen of GFP. Similar parts have been used in previous project (iGEM 2017 Fudan). We received the plasmids with the gene of cell surface-expressed GFP (标注复旦Part名） and synNotch (标注复旦Part名） from iGEM 2018 Fudan team. But the intracellular domain of synNotch is tTA, a kind of activation factor. Since synNotch was applied to the transformation of cells, the intracellular domain has been replaced by transcription activator factors such as GAL - VP64 and tTA [2]. However, promoters are not completely non-expressed until they are activated, and they often have background expression. Moreover, we hope to make some changes to the intracellular domain of synNotch, trying to replace the intracellular domain with non-traditional transcriptional activator factor to broaden the selection and application of synNotch intracellular domain.
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5.You can search for blog posts in one or more keywords in the app, and sort the search results by time, number of view , and collection. You can also search for users or teams.
  
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We found that the previous team iGEM 2017 Oxford modified tetR by replacing the domain between tetR DNA binding domain and regulatory core domain with TEV enzyme cleavage site, so that tetR will be destroyed in the presence of TEV, losing the function of repressing promoter after tetO sequence and opening up the expression of downstream genes.
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6.You can follow the teams or users that you think are worthy of attention so that you can view their published content more easily in the future.
  
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According to the idea of iGEM 2017 Oxford, we replaced the intracellular domain of synNotch with TEV and repeated the function verification of synNotch to explore whether replacing intracellular domain with TEV will affect the function of synNotch.
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7.Each user can create or join a team, become a member, and use their team space. The team creator is the captain who can edit the team information in the team space for others to join and show the team research direction. Only members of the team can post in the team space, but everyone can view it. Also, the captain can add albums in the team space,where members of the team can post photos.
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8.This app has built-in gadgets that may be needed for the lab and more other features will be added in the future...
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What it could be
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An information gathering platform:
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We will gather information from other website to release, and we hope to build contact with some organization to get instant and accurate information. The user of this app can share their information in it. If it is valuable and reliable, we will push it to everyone.
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A platform for interaction between teams:
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Through the form of the team space, each team can easily interact with other teams. This will strengthen the connection between iGEM teams and cause countless positive possibilities.
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An iGEM community:
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Each iGEMer could leave a footprint here and show themselves here. Everyone here is part of the iGEM.
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The App we conceived can provide comprehensive information on the release of events, each team to register their own team home page, various activities related to iGEM, question answer and experience sharing , topic discussion, experimental supplies, and more.

Latest revision as of 10:05, 17 October 2018

Overview We developed iGEMCloud to solve some of iGEM's current problems, with its immediacy, openness and interactivity. At present, all the information about iGEM comes from many websites. They are scattered, cluttered and unfriendly to personal user.

So we have created this application(So far, it is android based, and we hope to launch the iOS version in the future) , which is hoped to be a information gathering platform that receives the latest information of iGEM, a micro-blog that allows all users to participate in discussions in group or individual, and a community that built for iGEMers all around the world.

Instructions 1.Register an account with your email address, enter the verify code, and use this address to log in.

2.In the application, you can receive official news, notifications, etc. And so that you can get the latest news of iGEM events in a timely, convenient and accurate way.

3.Users can write blog posts in the discussion forum for all users to browse. The blog post is in the form of pictures + text. The blog posts are classified into different sections, such as experiment, seek help, etc. And so that you can share your ideas with all iGEM participants.

Excellent blog posts will be tagged so that they are discovered by users. At the same time, users who see the blog posts written by you can comment or reply to you. The content of the reply is in the form of image + text. When you find a nice blog post, click on the star symbol in the upper right to bookmark it for later reading.

4.If you want to remind specific users to view the content you have posted, you can remind the user to view this blog post or comment by "@" + "user nickname".

5.You can search for blog posts in one or more keywords in the app, and sort the search results by time, number of view , and collection. You can also search for users or teams.

6.You can follow the teams or users that you think are worthy of attention so that you can view their published content more easily in the future.

7.Each user can create or join a team, become a member, and use their team space. The team creator is the captain who can edit the team information in the team space for others to join and show the team research direction. Only members of the team can post in the team space, but everyone can view it. Also, the captain can add albums in the team space,where members of the team can post photos.

8.This app has built-in gadgets that may be needed for the lab and more other features will be added in the future...

What it could be An information gathering platform: We will gather information from other website to release, and we hope to build contact with some organization to get instant and accurate information. The user of this app can share their information in it. If it is valuable and reliable, we will push it to everyone.

A platform for interaction between teams: Through the form of the team space, each team can easily interact with other teams. This will strengthen the connection between iGEM teams and cause countless positive possibilities.

An iGEM community: Each iGEMer could leave a footprint here and show themselves here. Everyone here is part of the iGEM.

The App we conceived can provide comprehensive information on the release of events, each team to register their own team home page, various activities related to iGEM, question answer and experience sharing , topic discussion, experimental supplies, and more.