Difference between revisions of "Team:NAU-CHINA/testh"

 
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Figure5. Function verification of recombinases in HEK 293T cell
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Overview
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Fluorescence microscope observation of HEK 293 T transfect with plasmids containing the recombinase recognition sites and corresponding recombinase gene.
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We developed iGEMCloud to solve some of iGEM's current problems, with its immediacy, openness and interactivity. At present, all the information about iGEM comes from many websites. They are scattered, cluttered and unfriendly to personal user.
  
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The image under fluorescence microscope for 293T cells, transfected with plasmids containing the recombinase recognition sites (upper panel) or transfected with plasmids containing corresponding recombinase gene together (bottom panel), are shown.
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So we have created this application(So far, it is android based, and we hope to launch the iOS version in the future) , which is hoped to be a information gathering platform that receives the latest information of iGEM, a micro-blog that allows all users to participate in discussions in group or individual, and a community that built for iGEMers all around the world.
  
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The results show that the recombinases can recognise the sites and reverse the sequence between sites in HEK 293T.
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Instructions
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1.Register an account with your email address, enter the verify code, and use this address to log in.
  
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Function verification of reversal efficiency and threshold characteristics of different recombinase in HEK 293 T Cells
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2.In the application, you can receive official news, notifications, etc. And so that you can get the latest news of iGEM events in a timely, convenient and accurate way.
  
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Fig.6. Function verification of recombinases in HEK 293T cell
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3.Users can write blog posts in the discussion forum for all users to browse. The blog post is in the form of pictures + text. The blog posts are classified into different sections, such as experiment, seek help, etc. And so that you can share your ideas with all iGEM participants.
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HEK 293T cells were co-transfected with six different amounts of plasmids containing recombinase genes (miniCMV-Bxb1 and miniCMV-TP901) , and fixed numbers of plasmids containing corresponding recombinase recognition sites. After 36 hours of plasmid co-transfection, the proportion of fluorescent cells and the average fluorescence intensity of cells were detected by flow cytometry. Transfection of different amounts of plasmids containing recombinase genes into cells indicates that cells can produce recombinase at different concentrations. The experiment was repeated three times.
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(A) Fluorescence microscope observation of HEK 293T undergone different experimental treatments
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(B) The statistical chart of average fluorescence intensity of cells shows that the cells with Bxb1 recombinase have a higher fluorescence intensity than those with TP901 recombinase under the same promoter strength and recombinase concentration. However, if the concentration of recombinase is low, there is no significant difference in fluorescence intensity.
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(C) The statistics of the proportion of fluorescent cells show that the proportion of fluorescent cells has a sudden jump discontinuity between low concentration and high concentration of Bxb1 and TP901 recombinases. Similar results were obtained in all three repetitions.
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The results of image B show that the reverse efficiency of Bxb1 recombinase is higher than TP901 recombinase under the same promoter strength and recombinase concentration. However, if the concentration of recombinase is low, there is no significant difference in fluorescence intensity. The results of the image C show that Bxb1 and TP901 recombinases have a threshold property. So, the proportion of fluorescent cells have a jump discontinuity between low concentration and high concentration of recombinase.
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Function verification of RDF in HEK 293T cell
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Excellent blog posts will be tagged so that they are discovered by users. At the same time, users who see the blog posts written by you can comment or reply to you. The content of the reply is in the form of image + text. When you find a nice blog post, click on the star symbol in the upper right to bookmark it for later reading.
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4.If you want to remind specific users to view the content you have posted, you can remind the user to view this blog post or comment by "@" + "user nickname".
 +
 
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5.You can search for blog posts in one or more keywords in the app, and sort the search results by time, number of view , and collection. You can also search for users or teams.
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6.You can follow the teams or users that you think are worthy of attention so that you can view their published content more easily in the future.
 +
 
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7.Each user can create or join a team, become a member, and use their team space. The team creator is the captain who can edit the team information in the team space for others to join and show the team research direction. Only members of the team can post in the team space, but everyone can view it. Also, the captain can add albums in the team space,where members of the team can post photos.
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8.This app has built-in gadgets that may be needed for the lab and more other features will be added in the future...
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What it could be
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An information gathering platform:
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We will gather information from other website to release, and we hope to build contact with some organization to get instant and accurate information. The user of this app can share their information in it. If it is valuable and reliable, we will push it to everyone.
 +
 
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A platform for interaction between teams:
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Through the form of the team space, each team can easily interact with other teams. This will strengthen the connection between iGEM teams and cause countless positive possibilities.
 +
 
 +
An iGEM community:
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Each iGEMer could leave a footprint here and show themselves here. Everyone here is part of the iGEM.
 +
 
 +
The App we conceived can provide comprehensive information on the release of events, each team to register their own team home page, various activities related to iGEM, question answer and experience sharing , topic discussion, experimental supplies, and more.

Latest revision as of 10:05, 17 October 2018

Overview We developed iGEMCloud to solve some of iGEM's current problems, with its immediacy, openness and interactivity. At present, all the information about iGEM comes from many websites. They are scattered, cluttered and unfriendly to personal user.

So we have created this application(So far, it is android based, and we hope to launch the iOS version in the future) , which is hoped to be a information gathering platform that receives the latest information of iGEM, a micro-blog that allows all users to participate in discussions in group or individual, and a community that built for iGEMers all around the world.

Instructions 1.Register an account with your email address, enter the verify code, and use this address to log in.

2.In the application, you can receive official news, notifications, etc. And so that you can get the latest news of iGEM events in a timely, convenient and accurate way.

3.Users can write blog posts in the discussion forum for all users to browse. The blog post is in the form of pictures + text. The blog posts are classified into different sections, such as experiment, seek help, etc. And so that you can share your ideas with all iGEM participants.

Excellent blog posts will be tagged so that they are discovered by users. At the same time, users who see the blog posts written by you can comment or reply to you. The content of the reply is in the form of image + text. When you find a nice blog post, click on the star symbol in the upper right to bookmark it for later reading.

4.If you want to remind specific users to view the content you have posted, you can remind the user to view this blog post or comment by "@" + "user nickname".

5.You can search for blog posts in one or more keywords in the app, and sort the search results by time, number of view , and collection. You can also search for users or teams.

6.You can follow the teams or users that you think are worthy of attention so that you can view their published content more easily in the future.

7.Each user can create or join a team, become a member, and use their team space. The team creator is the captain who can edit the team information in the team space for others to join and show the team research direction. Only members of the team can post in the team space, but everyone can view it. Also, the captain can add albums in the team space,where members of the team can post photos.

8.This app has built-in gadgets that may be needed for the lab and more other features will be added in the future...

What it could be An information gathering platform: We will gather information from other website to release, and we hope to build contact with some organization to get instant and accurate information. The user of this app can share their information in it. If it is valuable and reliable, we will push it to everyone.

A platform for interaction between teams: Through the form of the team space, each team can easily interact with other teams. This will strengthen the connection between iGEM teams and cause countless positive possibilities.

An iGEM community: Each iGEMer could leave a footprint here and show themselves here. Everyone here is part of the iGEM.

The App we conceived can provide comprehensive information on the release of events, each team to register their own team home page, various activities related to iGEM, question answer and experience sharing , topic discussion, experimental supplies, and more.