Difference between revisions of "Team:NAU-CHINA/testh"

 
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Fig.7. Function verification of the reversal ability of recombinase-RDFs in HEK 293 T Cells
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Overview
Fluorescence microscope observation of HEK 293 T transfect with plasmids containing the recombinase-RDF recognition sites and corresponding recombinase-RDF gene. The image under fluorescence microscope for 293T cells, transfected with plasmids containing the recombinase recognition sites (upper panel) or transfected with plasmids containing corresponding recombinase gene together (bottom panel), are shown.
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We developed iGEMCloud to solve some of iGEM's current problems, with its immediacy, openness and interactivity. At present, all the information about iGEM comes from many websites. They are scattered, cluttered and unfriendly to personal user.
  
The results show that the recombinase-RDFs can recognise the sites and reverse the sequence between sites in HEK 293T.
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So we have created this application(So far, it is android based, and we hope to launch the iOS version in the future) , which is hoped to be a information gathering platform that receives the latest information of iGEM, a micro-blog that allows all users to participate in discussions in group or individual, and a community that built for iGEMers all around the world.
  
Conclusion
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Instructions
We verified the functions of most parts and most upstream and downstream paths step by step. We verified the function of synNotch, the inhibition of tetR after modification, the reversal function and threshold characteristics of some recombinase and promoter combinations. However, due to time constraints, we are unable to complete verification of TEV and the combinations of some recombinases and promoters. Moreover, the combination of upstream and downstream circuits needs to be verified by experiments. We will carry out supplementary experiments in the future to carry out a complete experimental verification of our subject.
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1.Register an account with your email address, enter the verify code, and use this address to log in.
  
Future experiments
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2.In the application, you can receive official news, notifications, etc. And so that you can get the latest news of iGEM events in a timely, convenient and accurate way.
In a short period of one year, it is not easy to fully realize such a complex idea. Therefore, we have envisaged the next series of experiments to further realize our subject idea, combining the idea of continuous feedback between modeling and wet lab to ensure the best system.
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1. Optimized function verification of TEV suppressing tetR Inhibition
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3.Users can write blog posts in the discussion forum for all users to browse. The blog post is in the form of pictures + text. The blog posts are classified into different sections, such as experiment, seek help, etc. And so that you can share your ideas with all iGEM participants.
As mentioned earlier, since the reporter gene selected GFP, our experimental results are not intuitive. We will replace the reporter gene with RFP to solve this problem.
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2. Verification of the combinations of remaining recombinases and promoters
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Excellent blog posts will be tagged so that they are discovered by users. At the same time, users who see the blog posts written by you can comment or reply to you. The content of the reply is in the form of image + text. When you find a nice blog post, click on the star symbol in the upper right to bookmark it for later reading.
We plan to continue the experiment of remaining combinations that have not yet been verified in order to verify the function and threshold characteristics of these combinations of recombinases and compare the inversion efficiency of recombinases.
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3. Construction of a fully functional stable cell line combining upstream and downstream circuits
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4.If you want to remind specific users to view the content you have posted, you can remind the user to view this blog post or comment by "@" + "user nickname".
We plan to finally construct our parts on two plasmids. Stable cell lines with complete functions were constructed through Puro and BSD screening and their concentration threshold functions will be verified by using agarose beads with different amounts of GFP adsorbed. We intend to apply it to real life.
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4. Upgrade our system
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5.You can search for blog posts in one or more keywords in the app, and sort the search results by time, number of view , and collection. You can also search for users or teams.
The above mentioned is only a condensed version of our ultimate system which include inhabitor and more efficient RDF. We hope to upgrade the condensed version to the final version, which also requires the search for appropriate inhabitor and more efficient RDF. We look forward to the day when our final version will come into being.
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Reference
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6.You can follow the teams or users that you think are worthy of attention so that you can view their published content more easily in the future.
[1] Circuits, C. A. et al. Precision Tumor Recognition by T Cells With Article Precision Tumor Recognition by T Cells With Combinatorial Antigen-Sensing Circuits. 1–10 (2016).
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[2] Morsut, L., Roybal, K. T., Xiong, X., Gordley, R. M. & Coyle, S. M. Engineering customized cell sensing and response behaviors using synthetic notch receptors. Cell 780–791 (2016). doi:10.1016/j.cell.2016.01.012
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7.Each user can create or join a team, become a member, and use their team space. The team creator is the captain who can edit the team information in the team space for others to join and show the team research direction. Only members of the team can post in the team space, but everyone can view it. Also, the captain can add albums in the team space,where members of the team can post photos.
  
[3] Rubens, J. R., Selvaggio, G. & Lu, T. K. Synthetic mixed-signal computation in living cells. Nat. Commun. 7, 1–10 (2016).
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8.This app has built-in gadgets that may be needed for the lab and more other features will be added in the future...
  
[4] Rutherford, K. & Van Duyne, G. D. The ins and outs of serine integrase site-specific recombination. Curr. Opin. Struct. Biol. 24, 125–131 (2014).
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What it could be
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An information gathering platform:
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We will gather information from other website to release, and we hope to build contact with some organization to get instant and accurate information. The user of this app can share their information in it. If it is valuable and reliable, we will push it to everyone.
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A platform for interaction between teams:
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Through the form of the team space, each team can easily interact with other teams. This will strengthen the connection between iGEM teams and cause countless positive possibilities.
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 +
An iGEM community:
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Each iGEMer could leave a footprint here and show themselves here. Everyone here is part of the iGEM.
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The App we conceived can provide comprehensive information on the release of events, each team to register their own team home page, various activities related to iGEM, question answer and experience sharing , topic discussion, experimental supplies, and more.

Latest revision as of 10:05, 17 October 2018

Overview We developed iGEMCloud to solve some of iGEM's current problems, with its immediacy, openness and interactivity. At present, all the information about iGEM comes from many websites. They are scattered, cluttered and unfriendly to personal user.

So we have created this application(So far, it is android based, and we hope to launch the iOS version in the future) , which is hoped to be a information gathering platform that receives the latest information of iGEM, a micro-blog that allows all users to participate in discussions in group or individual, and a community that built for iGEMers all around the world.

Instructions 1.Register an account with your email address, enter the verify code, and use this address to log in.

2.In the application, you can receive official news, notifications, etc. And so that you can get the latest news of iGEM events in a timely, convenient and accurate way.

3.Users can write blog posts in the discussion forum for all users to browse. The blog post is in the form of pictures + text. The blog posts are classified into different sections, such as experiment, seek help, etc. And so that you can share your ideas with all iGEM participants.

Excellent blog posts will be tagged so that they are discovered by users. At the same time, users who see the blog posts written by you can comment or reply to you. The content of the reply is in the form of image + text. When you find a nice blog post, click on the star symbol in the upper right to bookmark it for later reading.

4.If you want to remind specific users to view the content you have posted, you can remind the user to view this blog post or comment by "@" + "user nickname".

5.You can search for blog posts in one or more keywords in the app, and sort the search results by time, number of view , and collection. You can also search for users or teams.

6.You can follow the teams or users that you think are worthy of attention so that you can view their published content more easily in the future.

7.Each user can create or join a team, become a member, and use their team space. The team creator is the captain who can edit the team information in the team space for others to join and show the team research direction. Only members of the team can post in the team space, but everyone can view it. Also, the captain can add albums in the team space,where members of the team can post photos.

8.This app has built-in gadgets that may be needed for the lab and more other features will be added in the future...

What it could be An information gathering platform: We will gather information from other website to release, and we hope to build contact with some organization to get instant and accurate information. The user of this app can share their information in it. If it is valuable and reliable, we will push it to everyone.

A platform for interaction between teams: Through the form of the team space, each team can easily interact with other teams. This will strengthen the connection between iGEM teams and cause countless positive possibilities.

An iGEM community: Each iGEMer could leave a footprint here and show themselves here. Everyone here is part of the iGEM.

The App we conceived can provide comprehensive information on the release of events, each team to register their own team home page, various activities related to iGEM, question answer and experience sharing , topic discussion, experimental supplies, and more.