Difference between revisions of "Team:ZJU-China/Notebook"
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<ol> | <ol> | ||
<li style="text-align: left;"class="selected" data-date="31/12/2010"> | <li style="text-align: left;"class="selected" data-date="31/12/2010"> | ||
| − | |||
<em>End of Winter</em> | <em>End of Winter</em> | ||
<p>After the end of the winter selection, we formed a new team - ZJU-China 2018. It is the beginning of the story.</p> | <p>After the end of the winter selection, we formed a new team - ZJU-China 2018. It is the beginning of the story.</p> | ||
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<li data-date="30/07/2011"> | <li data-date="30/07/2011"> | ||
| − | |||
<em>March 8 - June 2</em> | <em>March 8 - June 2</em> | ||
<p style="font-size: .9em !important;">At the group meeting, everyone had brainstormed many times. This is a rather long process, many ideas were proposed and rejected, and finally, we reached a consensus - to create a cool artificial multi-enzyme complex</p> | <p style="font-size: .9em !important;">At the group meeting, everyone had brainstormed many times. This is a rather long process, many ideas were proposed and rejected, and finally, we reached a consensus - to create a cool artificial multi-enzyme complex</p> | ||
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<li data-date="31/11/2011"> | <li data-date="31/11/2011"> | ||
| − | |||
<em>June 15</em> | <em>June 15</em> | ||
<p>We went out to play and know each other better!</p> | <p>We went out to play and know each other better!</p> | ||
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<li data-date="31/01/2012"> | <li data-date="31/01/2012"> | ||
| − | |||
<em>June 23</em> | <em>June 23</em> | ||
<p>We officially confirmed the project as a protein scaffold and started the experiment.</p> | <p>We officially confirmed the project as a protein scaffold and started the experiment.</p> | ||
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<li data-date="30/04/2012"> | <li data-date="30/04/2012"> | ||
| − | |||
<em>June 25</em> | <em>June 25</em> | ||
<p>We divided our project into three parts. 1 - Curli expression. 2 - Enzyme construction 3 - Electrode modification. We prepared essential material and protocols for the the most important battlefield - Summer!</p> | <p>We divided our project into three parts. 1 - Curli expression. 2 - Enzyme construction 3 - Electrode modification. We prepared essential material and protocols for the the most important battlefield - Summer!</p> | ||
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<li data-date="31/01/2013"> | <li data-date="31/01/2013"> | ||
| − | |||
<em>July 13</em> | <em>July 13</em> | ||
<p>The summer vacation had begun. The csgA protein has been successfully expressed. However, the construction of csgA KO strains met some difficulties. Despite this, the staged results still make us very happy!</p> | <p>The summer vacation had begun. The csgA protein has been successfully expressed. However, the construction of csgA KO strains met some difficulties. Despite this, the staged results still make us very happy!</p> | ||
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</li> | </li> | ||
<li data-date="30/06/2013"> | <li data-date="30/06/2013"> | ||
| − | |||
<em>July 25</em> | <em>July 25</em> | ||
<p>We have successfully constructed recombination strains with exogenous protein. Since the expression of eukaryotic proteins in prokaryotic systems is indeed a challenge, we have many failures. However, after many attempts, we successfully completed the expected goal!</p> | <p>We have successfully constructed recombination strains with exogenous protein. Since the expression of eukaryotic proteins in prokaryotic systems is indeed a challenge, we have many failures. However, after many attempts, we successfully completed the expected goal!</p> | ||
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<li data-date="30/08/2013"> | <li data-date="30/08/2013"> | ||
| − | |||
<em>July 29</em> | <em>July 29</em> | ||
<p>We had a pleasant meeting with ACON Bio. After showing them the project, they feel that our project has great possibilities, but it needs to be improved in some practical aspects.</p> | <p>We had a pleasant meeting with ACON Bio. After showing them the project, they feel that our project has great possibilities, but it needs to be improved in some practical aspects.</p> | ||
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<li data-date="31/01/2014"> | <li data-date="31/01/2014"> | ||
| − | <h2> | + | <h2></h2> |
<em>August 6</em> | <em>August 6</em> | ||
<p>We went to the Zhejiang Science and Technology Museum and hoped to promote our own projects. In a cheerful atmosphere, we not only introduced them to the basic knowledge of synthetic biology, but also introduced some of our projects. To our surprise, the children have a very strong interest in our projects. A child even pointed out a problem that we ourselves are confused! What a surprising experience.</p> | <p>We went to the Zhejiang Science and Technology Museum and hoped to promote our own projects. In a cheerful atmosphere, we not only introduced them to the basic knowledge of synthetic biology, but also introduced some of our projects. To our surprise, the children have a very strong interest in our projects. A child even pointed out a problem that we ourselves are confused! What a surprising experience.</p> | ||
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<li data-date="30/04/2014"> | <li data-date="30/04/2014"> | ||
| − | |||
<em>August 14</em> | <em>August 14</em> | ||
<p style="font-size: .9em !important;">We have learned the electrode technology and decided to make two products - one is basic, using electrodes as well as electrochemical workstations, the other is smaller, and can even in a pocket.</p> | <p style="font-size: .9em !important;">We have learned the electrode technology and decided to make two products - one is basic, using electrodes as well as electrochemical workstations, the other is smaller, and can even in a pocket.</p> | ||
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<li data-date="31/06/2014"> | <li data-date="31/06/2014"> | ||
| − | |||
<em>August 18</em> | <em>August 18</em> | ||
<p>We had sought help from Shanghai Tech University and gotten a csgA-KO strain. With this bacterium, our project has taken a big step forward. By testing the KO strain, we verified the function of Curli and prepared to fix it to the electrode in the next step.</p> | <p>We had sought help from Shanghai Tech University and gotten a csgA-KO strain. With this bacterium, our project has taken a big step forward. By testing the KO strain, we verified the function of Curli and prepared to fix it to the electrode in the next step.</p> | ||
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<li data-date="31/11/2014"> | <li data-date="31/11/2014"> | ||
| − | |||
<em>August 25</em> | <em>August 25</em> | ||
<p>We went to CCIC. This is the Asian Forum on Synthetic Biology, and we showcased our projects. Other teams in china gave us many useful suggestions and had a lot of communication with us. Through CCIC, we knew everyone's progress and also clarified the subsequent tasks.</p> | <p>We went to CCIC. This is the Asian Forum on Synthetic Biology, and we showcased our projects. Other teams in china gave us many useful suggestions and had a lot of communication with us. Through CCIC, we knew everyone's progress and also clarified the subsequent tasks.</p> | ||
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<li data-date="31/12/2014"> | <li data-date="31/12/2014"> | ||
| − | |||
<em>August 26</em> | <em>August 26</em> | ||
<p>We have consulted some question about safety with Joichim messing，he gave us a lot of practical advises. Thanks so much for him! </p> | <p>We have consulted some question about safety with Joichim messing，he gave us a lot of practical advises. Thanks so much for him! </p> | ||
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<li data-date="31/05/2015"> | <li data-date="31/05/2015"> | ||
| − | |||
<em>September 3</em> | <em>September 3</em> | ||
<p>We basically completed the production of the first generation of products. The results given by the electrochemical workstation are very satisfactory. However, its large size limits the practicality of the product. At the same time, the development of the second generation of smaller and lighter products is nearing completion. We are going to get the final result!</p> | <p>We basically completed the production of the first generation of products. The results given by the electrochemical workstation are very satisfactory. However, its large size limits the practicality of the product. At the same time, the development of the second generation of smaller and lighter products is nearing completion. We are going to get the final result!</p> | ||
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</li> | </li> | ||
<li data-date="31/12/2015"> | <li data-date="31/12/2015"> | ||
| − | |||
<em>September 25</em> | <em>September 25</em> | ||
<p>We finally finished the final product! A beautiful interdigital electrode successfully distinguishes between the patient and the normal person. Although its stability needs to be further improved, this achievement is very exciting!</p> | <p>We finally finished the final product! A beautiful interdigital electrode successfully distinguishes between the patient and the normal person. Although its stability needs to be further improved, this achievement is very exciting!</p> | ||
| Line 583: | Line 573: | ||
</li> | </li> | ||
<li data-date="31/05/2016"> | <li data-date="31/05/2016"> | ||
| − | |||
<em>August 1 – 15</em> | <em>August 1 – 15</em> | ||
<p>After completing the final optimization of the product, we started writing wikis. Writing a wiki every day keeps doctor away.</p> | <p>After completing the final optimization of the product, we started writing wikis. Writing a wiki every day keeps doctor away.</p> | ||
Revision as of 10:24, 17 October 2018
-
End of Winter
After the end of the winter selection, we formed a new team - ZJU-China 2018. It is the beginning of the story.
-
March 8 - June 2
At the group meeting, everyone had brainstormed many times. This is a rather long process, many ideas were proposed and rejected, and finally, we reached a consensus - to create a cool artificial multi-enzyme complex
At the group meeting, everyone had brainstormed many times. This is a rather long process, many ideas were proposed and rejected, and finally, we reached a consensus - to create a cool artificial multi-enzyme complex!
-
June 15
We went out to play and know each other better!
-
June 23
We officially confirmed the project as a protein scaffold and started the experiment.
-
June 25
We divided our project into three parts. 1 - Curli expression. 2 - Enzyme construction 3 - Electrode modification. We prepared essential material and protocols for the the most important battlefield - Summer!
-
July 13
The summer vacation had begun. The csgA protein has been successfully expressed. However, the construction of csgA KO strains met some difficulties. Despite this, the staged results still make us very happy!
-
July 25
We have successfully constructed recombination strains with exogenous protein. Since the expression of eukaryotic proteins in prokaryotic systems is indeed a challenge, we have many failures. However, after many attempts, we successfully completed the expected goal!
-
July 29
We had a pleasant meeting with ACON Bio. After showing them the project, they feel that our project has great possibilities, but it needs to be improved in some practical aspects.
-
August 6
We went to the Zhejiang Science and Technology Museum and hoped to promote our own projects. In a cheerful atmosphere, we not only introduced them to the basic knowledge of synthetic biology, but also introduced some of our projects. To our surprise, the children have a very strong interest in our projects. A child even pointed out a problem that we ourselves are confused! What a surprising experience.
-
August 14
We have learned the electrode technology and decided to make two products - one is basic, using electrodes as well as electrochemical workstations, the other is smaller, and can even in a pocket.
Work on 2D printing is also progressing well. We made a practical 2D printer and prepared to use it to accurately print the enzyme onto the electrode.
-
August 18
We had sought help from Shanghai Tech University and gotten a csgA-KO strain. With this bacterium, our project has taken a big step forward. By testing the KO strain, we verified the function of Curli and prepared to fix it to the electrode in the next step.
-
August 25
We went to CCIC. This is the Asian Forum on Synthetic Biology, and we showcased our projects. Other teams in china gave us many useful suggestions and had a lot of communication with us. Through CCIC, we knew everyone's progress and also clarified the subsequent tasks.
-
August 26
We have consulted some question about safety with Joichim messing，he gave us a lot of practical advises. Thanks so much for him!
-
September 3
We basically completed the production of the first generation of products. The results given by the electrochemical workstation are very satisfactory. However, its large size limits the practicality of the product. At the same time, the development of the second generation of smaller and lighter products is nearing completion. We are going to get the final result!
-
September 25
We finally finished the final product! A beautiful interdigital electrode successfully distinguishes between the patient and the normal person. Although its stability needs to be further improved, this achievement is very exciting!
-
August 1 – 15
After completing the final optimization of the product, we started writing wikis. Writing a wiki every day keeps doctor away.
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