Difference between revisions of "Team:KCL UK"

 
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{{KCL_UK}}
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{{:Team:KCL_UK/Templates/Header}}
 
<html>
 
<html>
−
<div class="column full_size" >
+
    <head>
−
<h1> Welcome to the King’s College London 2018 Team Wiki! </h1>
+
−
<p> In our project we are developing a new technology platform based on the knowledge of the role of sRNAs in bacteria to control antibiotic resistance. We will use E.coli K12 to test our strategies by engineering libraries of sRNA scaffolds  to modulate gene expression patents in relation to the external environmental factors.</p>
+
  
−
<img src="https://static.igem.org/mediawiki/2018/f/f5/T--KCL_UK--kclteampic.png">
+
    <meta charset="UTF-8">
−
+
    <meta name="viewport" content="width= device-width, initial-scale=1">
−
</div>  
+
    <title>KCL iGEM</title>
  
−
<div class="column full_size" >
 
−
<h3>What are we Doing?</h3>
 
−
<p>We are developing a new technology platform based on 3'UTR sequence characterisation to control soluble protein expression in E.coli. </br></br>
 
−
We will use E.coli K12 to test our "Proof of Concept" Principle by engineering 3'UTR sequences (Sharma et al, 2011) to interact with either cytosolic proteins or 5'UTR sequences of our gene of interest: trxA and gor. Down regulation of these genes will be quantified by assessing quantitatively GFP signal. Efficiency of down regulation of genes will be quantified by assessing reaction kinetics of Alkaline Phosphatase (AP).</p>
 
−
<div class="button_link">
 
−
<a href="https://2018.igem.org/Team:KCL_UK/Description">
 
−
FULL PROJECT DESCRIPTION</a>
 
−
</div>
 
  
−
<div class="clear extra_space"></div>
+
    <style>  
−
<div class="line_divider"></div>
+
    @import url('https://fonts.googleapis.com/css?family=Raleway');
−
<div class="clear extra_space"></div>
+
  
−
<div class="column full_size" >
+
    #top_title {display:none;}
−
<h2>See Our Work</h2>
+
   
  
  
−
</div>
+
  #HQ_page p {
 +
font-family: 'Raleway', sans-serif;
 +
font-size: 1em;
 +
text-align: center;
  
−
<div class="clear extra_space"></div>
+
}
 +
   
 +
    #HQ_page h1,h2,h3 {
 +
font-family: 'Raleway', sans-serif;
 +
font-weight: 300;
 +
}
  
−
<div class="column third_size" >
+
      #content, #HQ_page {
−
<div class="highlight decoration_A_full">
+
            animation : none;
−
<h3 style="font-size:1.5em;">Education and Outreach</h3>
+
    animation-delay : 0;
−
<p>As a team, we strive to reach out to all communities to share knowledge which not only empowers people of all ages, but also shares what we have learned as an iGEM team doing research in synthetic biology.</p>
+
    animation-direction : normal;
−
<p>We hold many events such as school visits and iGEM symposiums, you can read more in the Human Practices section!</p>
+
    animation-duration : 0;
−
<div class="button_link">
+
    animation-fill-mode : none;
−
<a href="https://2018.igem.org/Team:KCL_UK/Public_Engagement">
+
    animation-iteration-count : 1;
−
SEE MORE
+
    animation-name : none;
−
</a>
+
    animation-play-state : running;
−
</div>
+
    animation-timing-function : ease;
−
</div>
+
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−
</div>
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 +
    quotes : '\201C' '\201D' '\2018' '\2019';
 +
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 +
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      }
  
−
<div class="column third_size" >
+
h1 {
−
<div class="highlight decoration_A_full">
+
display: block;
−
<h3>Lab Work</h3>
+
font-size: 2em;
−
<p>We document and record everything we do in the lab, so anyone can look into the research which we have been working on. In the Lab Work section you can read all about our process, testing, and model.</p>
+
margin-bottom: 0.67em;
−
<div class="button_link">
+
margin-left: 0;
−
<a href="https://2018.igem.org/Team:KCL_UK/Experiments">
+
margin-right: 0;
−
SEE MORE
+
  }
−
</a>
+
−
</div>
+
−
</div>
+
−
</div>
+
  
−
<div class="column third_size" >
+
  a:hover {text-decoration:none;}
−
<div class="highlight decoration_A_full">
+
a:visited {
−
<h3>Social Media Engagement</h3>
+
  color:red;
−
<p>We make sure to update frequently on our social media pages. This allows other teams to see what we've been up to and have some fun!</p>
+
  text-decoration:none;
 +
}
  
−
<a href="https://www.facebook.com/igemkcl/">
+
  .dropdown-nav-buttonmasks:visited {
−
<img style="height:60px;width:60px;" src="https://static.igem.org/mediawiki/2018/9/95/T--KCL_UK--fblogo.png">
+
    color:white;
−
</a>
+
      text-decoration: none;
−
<a href="https://www.instagram.com/igem_kcl/">
+
    }
−
<img style="height:60px;width:60px;" src="https://static.igem.org/mediawiki/2018/7/71/T--KCL_UK--iglogo.png">
+
.standalonenav:visited {
−
</a>
+
  color:white;
−
<a href="https://twitter.com/kcligem?lang=en">
+
  text-decoration: none;
−
<img style="height:60px;width:60px;" src="https://static.igem.org/mediawiki/2018/e/ee/T--KCL_UK--twitterlogo.png">
+
}
−
</a>
+
  
 +
 
  
−
</div>
+
        * {
−
</div>
+
            box-sizing: border-box;
−
<!--
+
            font-family: 'Raleway', sans-serif;
 +
        }
  
−
<div class="clear extra_space"></div>
 
−
<div class="line_divider"></div>
 
−
<div class="clear extra_space"></div>
 
  
−
<div class="column two_thirds_size" >
+
        body{
−
<h3></h3>
+
        margin: 0;
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
    padding: 0;
−
<ul>
+
        font-family: 'Raleway', sans-serif;
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
        text-align: center;
−
<li>Be clear about what you are doing and how you plan to do this.</li>
+
        }
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
 
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
        img {
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
+
            max-width: 100%;
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
+
            height: auto;
−
<li>Have lots of fun! </li>
+
        }
−
</ul>  
+
 
−
</div>
+
        .container {
 +
            width: 95%;
 +
            margin: 0 auto;
 +
        }
 +
        .fixcontainer {
 +
          width:100%;
 +
          margin: 0 auto;
 +
        }
 +
 
 +
        /*typography------------------------------*/
 +
        .title {
 +
            font-weight: 900;
 +
            font-weight: bold;
 +
            font-size: 3.2rem;
 +
            margin-bottom: 1em;
 +
            padding-bottom: 2em;
 +
            text-emphasis: bold;
 +
        }
 +
        .smallertitle {
 +
          font-weight: 900;
 +
          font-weight: bold;
 +
          font-size: 1.4rem;
 +
          margin-bottom: 1em;
 +
          padding-top: 3.1em;
 +
          padding-bottom: 0em;
 +
          text-emphasis: bold;
 +
 
 +
        }
 +
        h1 {
 +
            font-weight: 300;
 +
            font-size: 2.5em;
 +
            text-transform: capitalize;
 +
          }
 +
        .title span {
 +
            margin-top: 1em;
 +
            font-weight: 200;
 +
            display: block;
 +
            font-size: .9em;
 +
        }
 +
 
 +
 
 +
        /*buttonmasks -------------------------------*/
 +
        .buttonmask {
 +
            display: inline-block;
 +
            font-size: 1.25rem;
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            text-decoration: none;
 +
            text-transform: uppercase;
 +
            border-width: 2px;
 +
            border-style: solid;
 +
            padding: .5em 1.75em;
 +
 
 +
        }
 +
  .buttonmask}
 +
 
 +
        .buttonmask-small {
 +
            font-size: .9rem;
 +
            font-weight: 700;
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        }
 +
 
 +
        .buttonmask-accent {
 +
            color: #FF0000;
 +
            border-color: #FF0000;
 +
        }
 +
 
 +
        .buttonmask-accent:hover {
 +
        background: #FF0000;
 +
        color: black;
 +
        }
 +
 
 +
 
 +
        /*header------------------------------*/
 +
 
 +
        header {
 +
            background-color: rgba(0,0,0,.75);
 +
            position: absolute;
 +
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 +
 
 +
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 +
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            padding: 0;
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            margin-right: 5em!important;
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            position: relative;
 +
        }
 +
 
 +
        nav li {
 +
            display: inline-block;
 +
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 +
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 +
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 +
            padding: .5em;
 +
            color: #FFF;
 +
            position: relative;
 +
            padding-bottom: 3em;
 +
        }
 +
 
 +
        .nav-buttonmasks:hover {color: #FF0000;}
 +
        .dropdown-nav-buttonmasks:hover {color: #FF0000;}
 +
 
 +
        .dropdown-nav-buttonmasks {
 +
            font-weight: 900;
 +
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 +
            text-transform: uppercase;
 +
            font-size: 0.95rem;
 +
            padding: .5em;
 +
            color: #FFF;
 +
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            z-index: 1;
 +
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 +
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 +
        #navadjust {
 +
          margin-left:-0.3em;
 +
        }
 +
 
 +
     
 +
 
 +
        .nav-buttonmasks:hover .dropdown-nav-buttonmasks{
 +
            display: block;
 +
            position: absolute;
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 +
        .logo {
 +
            height: 90px;
 +
            width: 90px;
 +
            float: left;
 +
            margin-left: 6em;
 +
            margin-top: .5em;
 +
        }
 +
 
 +
          /*home hero------------------------------*/
 +
 
 +
        .home-hero {
 +
            background-image: url(https://static.igem.org/mediawiki/2018/e/ed/T--KCL_UK--homehero.png);
 +
            background-repeat: no-repeat;
 +
            background-size: cover;
 +
            padding: 10em 0;
 +
            color: #FFF;
 +
            height: 100vh;
 +
            background-attachment: fixed;
 +
            background-position: center;
 +
        }
 +
 
 +
        #seeourwork {
 +
          margin-top: 0em;
 +
          margin-bottom: 2em;
 +
        }
 +
 
 +
  /*home-about------------------------------*/
 +
        .home-about-textbox {
 +
            background-color: black;
 +
            padding: 15em;
 +
 
 +
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 +
            outline: 2px solid #FF0000;
 +
            outline-offset: -5em;
 +
            color: #FFF;
 +
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 +
 
 +
        .home-about-textbox h1 {
 +
            color: #FF0000;
 +
            position: relative;
 +
            margin-top: -5em;
 +
            margin-bottom: 3em;
 +
            background: black;
 +
            margin-left: auto;
 +
            margin-right: auto;
 +
            width: 35vw;
 +
            text-align: center;
 +
       
 +
        }
 +
        .home-about-textbox a {
 +
            margin-top: 2em;
 +
            margin-bottom: 0;
 +
            width: 60%;
 +
        }
 +
 
 +
        #projectdescshort {
 +
          color: white;
 +
font-size: 1.4em!important;
 +
margin-top: 2em!important;
 +
padding-top:2em!important;
 +
         
 +
     
 +
        }
 +
 
 +
 
 +
 
 +
      /*portfolio------------------------------*/
 +
 
 +
        .portfolio h1 {
 +
            width: 12em;
 +
            margin: auto;
 +
            margin-top: -1.5em;
 +
            padding-bottom: .7em;
 +
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 +
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 +
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 +
        .line {
 +
                width: 30%;
 +
                height: 1px;
 +
                margin: auto;
 +
            background: #FF0000;
 +
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 +
        .spacer {
 +
            width: 100%;
 +
            height: 2em;
 +
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 +
        .portfolio {
 +
            margin: 3em 0;
 +
            background-color: black;
 +
 
 +
        }
 +
 
 +
        .port-item {
 +
            position: relative;
 +
            margin: 0;
 +
            width: 50%;
 +
            float: left;
 +
        }
 +
        .port-item img {
 +
            display: block;
 +
            width: 100%;
 +
            height: 15em;
 +
            object-fit: cover;
 +
 
 +
        }
 +
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        #projectdescshort {
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    <body>
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  <div class="loading">
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  <div>
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    <section class="home-hero">
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        <div class="container">
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          <!--
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        <h1 class="title titleadjust">3' Suspects
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            <span><h1 class="smallertitle">Investigating Untranslated regions on 3' end</h1></span>
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        </h1>
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        <a href="#" class="buttonmask buttonmask-accent" id="seeourwork">See Our Work</a>
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        </div>
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        --->
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    </section>
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    <section class="home-about">
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        <div class="home-about-textbox">
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            <h1>What Have We Done?</h1>
 +
            <p id="projectdescshort">Our team have engineered a library of sRNA scaffolds that have the potential to be used as a tool in the understanding of sRNA functions such as regulation of protein expression. </br></br>
 +
 
 +
Our project is based on a “Proof of Concept” principle and we have used E.coli K12 to test this. Regulation of gene expression has been quantitively assessed through GFP signalling. We have chosen to focus on sRNAs that target the 3’ UTR of mRNA.</br></br>
 +
 
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We hope that future scientists will be able to use our novel tool as a way to use sRNAs to tackle issues involving sRNA interactions such as antibiotic resistance.
 +
 
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    </p>
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            <a href="https://2018.igem.org/Team:KCL_UK/Description" class= "buttonmask buttonmask-accent">Full Project Description</a>
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        </div>
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    </section>
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        </div>
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    <section class="portfolio">
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        <h1>Some of Our Work</h1>
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        <div class="line"></div>
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        <div class="spacer"></div>
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        <!-- portfolio item 1 -->
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        <figure class="port-item">
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            <img src="https://static.igem.org/mediawiki/2018/f/fe/T--KCL_UK--symposium.png" alt="portfolio item">
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            <figcaption class="port-desc">
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                <p>Integrated Human Practices</p>
 +
                <a href="https://2018.igem.org/Team:KCL_UK/Human_Practices" class="buttonmask buttonmask-accent buttonmask-small">See More</a>
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            </figcaption>
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        </figure>
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 +
        <!-- portfolio item 2 -->
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        <figure class="port-item">
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            <img src="https://static.igem.org/mediawiki/2018/b/b7/T--KCL_UK--school.png" alt="portfolio item">
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            <figcaption class="port-desc">
 +
                <p>Bexley School Visit</p>
 +
                <a href="https://2018.igem.org/Team:KCL_UK/Public_Engagement" class="buttonmask buttonmask-accent buttonmask-small">See More</a>
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            </figcaption>
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        </figure>
 +
 
 +
        <!-- portfolio item 3 -->
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        <figure class="port-item">
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            <img src="https://static.igem.org/mediawiki/2018/8/80/T--KCL_UK--instagram.png" alt="portfolio item">
 +
            <figcaption class="port-desc">
 +
                <p>Social Media</p>
 +
                <a href="https://www.instagram.com/igem_kcl/" class="buttonmask buttonmask-accent buttonmask-small">See More</a>
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            </figcaption>
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        </figure>
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        <!-- portfolio item 4 -->
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        <figure class="port-item">
 +
            <img src="https://static.igem.org/mediawiki/2018/0/0a/T--KCL_UK--lab.png" alt="portfolio item">
 +
            <figcaption class="port-desc">
 +
                <p>Lab Work</p>
 +
                <a href="https://2018.igem.org/Team:KCL_UK/Design" class="buttonmask buttonmask-accent buttonmask-small">See More</a>
 +
            </figcaption>
 +
        </figure>
 +
    </section>
 +
 
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    <footer>
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    </footer>
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    </body>
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<div class="highlight decoration_A_full">
 
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<h3>Inspiration</h3>
 
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
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<ul>
 
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
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</ul>
 
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</div>
 
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</div>
 
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Latest revision as of 11:12, 17 October 2018

KCL iGEM

KCL iGEM

What Have We Done?

Our team have engineered a library of sRNA scaffolds that have the potential to be used as a tool in the understanding of sRNA functions such as regulation of protein expression.

Our project is based on a “Proof of Concept” principle and we have used E.coli K12 to test this. Regulation of gene expression has been quantitively assessed through GFP signalling. We have chosen to focus on sRNAs that target the 3’ UTR of mRNA.

We hope that future scientists will be able to use our novel tool as a way to use sRNAs to tackle issues involving sRNA interactions such as antibiotic resistance.

Full Project Description

Some of Our Work

portfolio item

Integrated Human Practices

See More
portfolio item

Bexley School Visit

See More
portfolio item

Social Media

See More
portfolio item

Lab Work

See More