Difference between revisions of "Team:KCL UK/Safety"

 
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     <body>
 
     <body>
  
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    <header>
 
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        <img src="https://static.igem.org/mediawiki/2018/0/08/T--KCL_UK--kclfinallogo.gif" alt="logo" class="logo">
 
  
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        <nav>
 
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            <ul>
 
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                <li><a href="index.html" class="nav-buttonmasks">Home</a></li>
 
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                <li class="nav-buttonmasks">Team
 
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                  <a href="teammembers.html" class="dropdown-nav-buttonmasks dropdownone">Team Members</a>
 
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                    <a href="#" class="dropdown-nav-buttonmasks dropdowntwo">Collaborations</a>
 
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                </li>
 
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                <li class="nav-buttonmasks">Project
 
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                  <a href="#" class="dropdown-nav-buttonmasks dropdownone">Description</a>
 
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                  <a href="#" class="dropdown-nav-buttonmasks dropdowntwo">Design</a>
 
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                  <a href="#" class="dropdown-nav-buttonmasks dropdownthree">Results</a>
 
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                </li>
 
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                <li class="nav-buttonmasks">Parts
 
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                  <a href="#" class="dropdown-nav-buttonmasks dropdownone">Parts Overview</a>
 
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                  <a href="#" class="dropdown-nav-buttonmasks dropdowntwo">Basic Parts</a>
 
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                  <a href="#" class="dropdown-nav-buttonmasks dropdownthree">Composite Parts</a>
 
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                </li>
 
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                <a href="#" class="nav-buttonmasks">Safety</a>
 
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                <li class="nav-buttonmasks">Human Practices
 
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                  <a href="integratedhumanpractices.html" class="dropdown-nav-buttonmasks dropdownone" id="navadjust">Integrated HP</a>
 
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                  <a href="educationandengagement.html" class="dropdown-nav-buttonmasks dropdowntwo" id="navadjust">Education and Engagement</a>
 
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                </li>
 
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                <a href="acknowledgements.html" class="nav-buttonmasks">Acknowledgements</a>
 
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            </ul>
 
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        </nav>
 
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    </header>
 
  
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  <div class="loading">
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    <div class="uselessspacer"></div>
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  <div>
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 +
    <section class="top">
 +
      <h1>Safety</h1>
 +
      <div class="line"></div>
 +
    </section>
  
 +
    <section class="collabcontent">
 +
      <div class="collab" id="first">
 +
        <div class="firstdescription description">Laboratory safety is paramount in ensuring a productive work environment. Prior to entering the lab to begin our project, our lab team attended two laboratory safety presentations where they were briefed and trained in maintaining safe practice in the lab.</div>
 +
          <div class="line linedescadjust"></div>
 +
          <div class="seconddescription description">With regards to our project, The RNAs we used in our project are well categorised and do not pose a threat to the public nor our lab workers. Our model organism used, E. Coli is typically low risk, falling into ‘Risk group 1’. E. coli typically inhabits the human colon, via ingestion however under good lab safety practice, this shouldn’t be an issue. E. Coli bind the mucosal colon surface firstly by the production of specific glycocalyx or fimbriae from the bacterium surface. Glycocalyx bind specific lectins on the enterocyte lining of the human colon. E. coli K12 is defective in several cell wall characteristics.</div>
 +
          <div class="line linedescadjust"></div>
 +
          <div class="seconddescription description">It's outer membrane has a defective lipopolysaccharide core which interrupts attachment of O- antigen polysaccharide side chains. It does not contain glycocalyx needed to attach to mucosal surfaces of the human colon due to altered O -antigen properties and K12 do not express capsular (K) antigens which are important in colonization and virulence. (Kuhnert et al 1995) Therefore, cannot colonise the human colon under normal conditions and K12 cannot under normal conditions colonize the GI tract of animals, negative results noted in mice, pigs, chickens and calves.
 +
          </div>
 +
          <div class="line linedescadjust"></div>
 +
          <div class="seconddescription description">K12 has not shown to have negative effects on plants (U.S. Department of Agriculture) Therefore, K12 is very unlikely to pose a hazard to animals, plants or microorganisms. Our plasmids do not pose any threat in toxicity or harmfulness and are adequately safe for transport and storage.
 +
          </div>
  
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    <section class="home-hero">
 
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        <div class="container">
 
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          <!--
 
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        <h1 class="title titleadjust">3' Suspects
 
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            <span><h1 class="smallertitle">Investigating Untranslated regions on 3' end</h1></span>
 
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        </h1>
 
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        <a href="#" class="buttonmask buttonmask-accent" id="seeourwork">See Our Work</a>
 
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        </div>
 
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        --->
 
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    </section>
 
  
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    <div class="fullcontainer" id="fix">
 
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    <section class="home-about">
 
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        <div class="home-about-textbox">
 
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            <h1>what are we doing?</h1>
 
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            <p id="projectdescshort">We are developing a new technology platform based on 3'UTR sequence characterisation to control soluble protein expression in E.coli. <br> <br>We will use E.coli K12 to test our "Proof of Concept" Principle by engineering 3'UTR sequences (Sharma et al, 2011) to interact with either cytosolic proteins or 5'UTR sequences of our gene of interest: trxA and gor. Down regulation of these genes will be quantified by assessing quantitatively GFP signal. Efficiency of down regulation of genes will be quantified by assessing reaction kinetics of Alkaline Phosphatase (AP).</p>
 
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            <a href="#" class= "buttonmask buttonmask-accent">Full Project Description</a>
 
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        </div>
 
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    </section>
 
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        </div>
 
  
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    <section class="portfolio">
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      </div>
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        <h1>Some of Our Work</h1>
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        <div class="line"></div>
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        <div class="spacer"></div>
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        <!-- portfolio item 1 -->
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        <figure class="port-item">
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            <img src="https://static.igem.org/mediawiki/2018/f/fe/T--KCL_UK--symposium.png" alt="portfolio item">
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            <figcaption class="port-desc">
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                <p>iGEM Symposium</p>
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                <a href="" class="buttonmask buttonmask-accent buttonmask-small">See More</a>
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            </figcaption>
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        </figure>
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        <!-- portfolio item 2 -->
 
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                <p>Bexley School Visit</p>
 
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                <p>Lab Work</p>
 
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Latest revision as of 11:20, 17 October 2018

KCL iGEM

KCL iGEM

Safety

Laboratory safety is paramount in ensuring a productive work environment. Prior to entering the lab to begin our project, our lab team attended two laboratory safety presentations where they were briefed and trained in maintaining safe practice in the lab.
With regards to our project, The RNAs we used in our project are well categorised and do not pose a threat to the public nor our lab workers. Our model organism used, E. Coli is typically low risk, falling into ‘Risk group 1’. E. coli typically inhabits the human colon, via ingestion however under good lab safety practice, this shouldn’t be an issue. E. Coli bind the mucosal colon surface firstly by the production of specific glycocalyx or fimbriae from the bacterium surface. Glycocalyx bind specific lectins on the enterocyte lining of the human colon. E. coli K12 is defective in several cell wall characteristics.
It's outer membrane has a defective lipopolysaccharide core which interrupts attachment of O- antigen polysaccharide side chains. It does not contain glycocalyx needed to attach to mucosal surfaces of the human colon due to altered O -antigen properties and K12 do not express capsular (K) antigens which are important in colonization and virulence. (Kuhnert et al 1995) Therefore, cannot colonise the human colon under normal conditions and K12 cannot under normal conditions colonize the GI tract of animals, negative results noted in mice, pigs, chickens and calves.
K12 has not shown to have negative effects on plants (U.S. Department of Agriculture) Therefore, K12 is very unlikely to pose a hazard to animals, plants or microorganisms. Our plasmids do not pose any threat in toxicity or harmfulness and are adequately safe for transport and storage.