Difference between revisions of "Team:OUC-China/Team"

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<section class="box features">
 
<section class="box features">
 
<h2 class="major"><span>Team member</span></h2>
 
<h2 class="major"><span>Team member</span></h2>
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<h3></h3>
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<p>
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<h3></h3>
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<p>Here we are, we are OUC-iGEM. It has been 8 years since we first took part in iGEM in 2011. This time the team which is made up of 8 members pursues a more particular and creative aim. All the members met in May 2017 when we were unfamiliar with each other and knew nothing about synthetic biology. We struck to intergrate the related disciplines and during this time we became not only teammates but also good friends. We come from different colleges and have diverse specialties which ensure that we join our abilities and insights to make our goal clearer as efficiently as possible.</p>
+
&emsp;&emsp;Here we are, we are OUC-iGEM. It has been 8 years since we first took part in iGEM in 2011. This time the team which is made up of 8 members pursues a more particular and creative aim. All the members met in May 2017 when we were unfamiliar with each other and knew nothing about synthetic biology. We struck to intergrate the related disciplines and during this time we became not only teammates but also good friends. We come from different colleges and have diverse specialties which ensure that we join our abilities and insights to make our goal clearer as efficiently as possible.
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<p align="justify">
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<br /><br />
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<h3>Project design</h3>
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  <div class="tt" align="center">
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<br />&emsp;&emsp;Being responsible for our earth and ourselves is our attitude towards every
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  <img src="https://static.igem.org/mediawiki/2018/9/93/T--OUC-China--ZYQ.png" width="200"/> <br/><br/><br/>
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  <img src="https://static.igem.org/mediawiki/2018/7/77/T--OUC-China--CYJ.png"width="200"/>   <br/><br/><br/>
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experiment. In our project, all the parts we have utilized are selected from Risk Group 1, none of
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  <img src="https://static.igem.org/mediawiki/2018/2/2f/T--OUC-China--HJC.png" width="200"/>
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</div>
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which are with a Red Flag. What’s more, nonpathogenic bacteria, E.coli DH5 Alpha is employed as the main
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  <div class="tt">
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  <h3>Yunqian Zhang</h3>
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chassis for our prior design on the DNA sequence, stem-loop and enzyme. As a matter of fact, when
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      <p>I am a senior in OUC whose major is biotechnology. To my surprise, I attend the iGEM competition in my last year of university. It is am amazing experience with my teammates to explore in the synthetic biology. Not only the lab work but also dry lab make me feel exciting and fresh.  </p>
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</div>
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using hairpins and csy4 enzyme to tune the expression of genes, it is relatively safe for  
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<div class="tt">
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      </div>
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industrialized applications and scientific research. Based on this, We designed more variants of csy4
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<div class="tt">
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      <h3>Yanju Chen</h3>
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enzymes and hairpins. （Click <a href='https://2018.igem.org/Team:OUC-China/Design'> here </a> to see more! ）So we used these to create a toolkit named miniToe family for synthetic biology which can
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      <p>Hello！I am YanjuChen, major in food science and engineering. It is my pleasure to meet some cute friends in OUC-iGEM team. For me, iGEM provides me a broader platform that helps me learn a lot about synthetic biology. Thank a fortunate encounter. Thank my dear friends. </p> </div>
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<div class="tt">
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control the expression of the target gene gradient. What’s more, we used the motA gene, one of the
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      <img src="https://static.igem.org/mediawiki/2018/2/2f/T--OUC-China--HJC.png" width="200"/> </div>
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<div class="tt">
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genes regulating the motility of E.coli, to verify the feasibility and effectiveness of the toolkit.  
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      <h3>Jiacheng Huang</h3>
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      <p>I'm an undergraduate from Institute of Economics who majors in Economics. In life, I love computer and math. In iGEM, I do mathematical modeling to help the project implementation. Hope to see you in jamboree!</p> </div>
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For the sake of environment, all the chassis organisms will be sterilized before being abandoned to  
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<div class="tt">
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      <img src="https://static.igem.org/mediawiki/2018/8/84/T--OUC-China--LXD.png" width="200"/> </div>
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prevent the genes from leaking.
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<div class="tt">
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      <h3>Xudong Liu</h3>
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      <p>I am Xudong Liu，a student in the Department of Biological Sciences. And I like to think about some mysteries of the universe and philosophical issues. I am mainly responsible for the experimental part of the team. </p> </div>
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<div class="tt">
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      <img src="https://static.igem.org/mediawiki/2018/7/7a/T--OUC-China--QQB.png" width="200"/> </div>
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<div class="tt">
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      <h3>QinBo Qiao</h3>
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      <p>Hay! I’m QinBo Qiao. My interest in synthetic biology stems from its unlimited potential for improvement. I have held on to the idea of it as my little joy to look forward to. When not iGEM-ing you will see me singing (or trying to), exploring this world by myself and controlling my weight of all time. See you guys in jamboree! </p> </div>
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<div class="tt">
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      <img src="https://static.igem.org/mediawiki/2018/c/c2/T--OUC-China--LQJ.png" width="200"/> </div>
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<div class="tt">
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      <h3>Qingjie Li</h3>
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      <p>This is Qingjie Li. Glad to participate in this competition! The days I spend in iGEM give me quantities of valuable experience, It’s lucky for me to have this chance to meet my teammates and work with them in this competition. I always eager to know somethings new and fancy as well I have always been longing for embracing new skills and theories. I find those in my life and in iGEM, It’s happy to be a iGEMer.
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</p> </div>
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<div class="tt">
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      <img src="https://static.igem.org/mediawiki/2018/4/4e/T--OUC-China--LAY.png" width="200"/> </div>
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<div class="tt">
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      <h3>Anyi Li</h3>
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      <p>I‘m Anyi Li，an undergraduate of College of Marines Life Science. In our team, mainly responsible for HP, and I also enjoy doing experiments with my teammates. It's a great pleasure to participate in this competition, to learn more knowledge as well as to meet more interesting people. I think it must  be a very special experience. </p> </div>
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<div class="tt">
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      <img src="https://static.igem.org/mediawiki/2018/c/c3/T--OUC-China--SK.png" width="200"/> </div>
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<div class="tt">
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      <h3>Kai Sun</h3>
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      <p>Hello everyone, my name is Kai.It's my pleasure to participate in iGEM. I have been interested in biological sciences and mystery novels since I was a child and think the two are very similar.I tried to find the secret of life, hoping to be like holmes. </div>
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</p>
 
</p>
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    </article>
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<p>
 +
<h3>Safe lab work</h3>
 +
<br />&emsp;&emsp;We works at a neat lab named “Lab for Microinnovation and Enterprise”, which is a
 +
 
 +
BSL-2 laboratory. Before working in the lab, we are supposed to be trained by the skillful hands, not
 +
 
 +
only for the general lab safety rules, but also for the standard experimental operation avoiding
 +
 
 +
unnecessary damage. No experiment carries no risk to the experimenters, hence we are properly equipped
 +
 
 +
with facilities, such as latex gloves, nitrile gloves, goggles, lab coats to protect us from biotic
 +
 
 +
and abiotic hazards.
 +
<br />
 +
<br />
 +
<div align="center"><img src="https://static.igem.org/mediawiki/2018/0/0d/T--OUC-China--safety001.jpg"
 +
 
 +
height="400" >
 +
                    <img src="https://static.igem.org/mediawiki/2018/9/9c/T--OUC-China--Safety01.jpg" 
 +
 
 +
height="400">
 +
</div>
 +
<br />
 +
<div align="center"><p style="font-size:80%;color:gray"">Fig.1 Our laboratory and personal safety
 +
 
 +
precautions</p></div>
 +
 
 +
&emsp;&emsp;Then for lab safety, a routine inspection is performed twice a day by everyone, not only
 +
 
 +
for ensuring normal operation of our lab like supplying experimental consumables and inspecting the
 +
 
 +
running of instruments, but also keeps it away from non-biological hazards, especially the water and
 +
 
 +
electricity. For high efficiency, responsibility implements to every team member.
 +
<br />
 +
<br />
 +
<div align="center"><img src="https://static.igem.org/mediawiki/2018/c/c2/T--OUC-China--safety002.jpg"
 +
 
 +
width="400" >
 +
                    <img src="https://static.igem.org/mediawiki/2018/2/2e/T--OUC-China--safety003.jpg"
 +
 
 +
width="400" >
 +
</div>
 +
<br /> <div align="center"><p style="font-size:80%;color:gray"">Fig.2 Table for routine inspection</p></div>
 +
 
 +
&emsp;&emsp;Furthermore, several operational zones are divided scientifically for special
 +
 
 +
experiments like A zone where is weighing the drugs, preparing the medium and sterilizing, B zone
 +
 
 +
where is the recording experimental note and organizing experimental data area, C zone where is a
 +
 
 +
molecular operation area, D zone where is RNA electrophoresis and a polyacrylamide gel electrophoresis
 +
 
 +
area.
 +
<br />&emsp;&emsp;For us, lab safety is a hot topic and we have come up with a practical lab safety
 +
 
 +
manual which normalizes our lovely lab.
 +
<br />
 +
<br />
 +
<h3>Practical lab safety manual</h3>
 +
<br />  1.Routine inspections must perform between 6:30-8:00pm and 9:30-11:00pm.
 +
<br />  2.Lab cleaning must be done twice a week with disinfectant moping the floor for at least 3times.
 +
<br />  3.Well prepared experiment plans should be written before you start.
 +
<br />  4.Lab coats and gloves must be worn when conducting experiments.
 +
<br />  5.Short skirts, shorts, and open shoes must not be worn.
 +
<br />  6.If you see a colleague doing something dangerous, point it out to him or her.
 +
<br />  7.Lab coats must not be worn outside laboratories and in public areas.
 +
<br />  8.Know where safety equipment (eyewash, shower and extinguisher) is located.
 +
<br />  9.Food/drink is not allowed in laboratories where chemicals are used stored.
 +
<br />  10.Always read the directions before using instruments and read MSDS before handling new
 +
 
 +
chemicals carefully.
 +
<br /> <br />
 +
<div align="center"><img src="https://static.igem.org/mediawiki/2018/3/38/T--OUC-China--Safety004.jpg"
 +
 
 +
height="400">
 +
                    <img src="https://static.igem.org/mediawiki/2018/c/cb/T--OUC-China--Safety006.jpg"
 +
 
 +
height="400" >
 +
</div>  <br />           
 +
<div align="center">
 +
<img src="https://static.igem.org/mediawiki/2018/d/d1/T--OUC-China--Safety005.jpg" width="840" >
 +
</div>
 +
<br /> <div align="center"><p style="font-size:80%;color:gray"">Fig.3 Safety operation</p></div>
 +
 
 +
<h3>Safe shipment</h3>
 +
<br />For submitting our parts to igem HQ, we first took the initiative to consult Chinese entry-exit inspection and quarantine laws and regulations about shipping DNA from China to the USA, which is forbidden without tedious red tape. Fortunately, GenScript cooperated with igem HQ forward our part sample to the USA. Ergo, we sealed dried DNA and sent it in the submission kit to GenScript, which clearly labelled as DNA without disguise. Our kit poses no risks and is perfectly safe for shipment.
 +
 
 +
<br />
 +
<br />
 +
<h3>Safety form</h3>
 +
<br />  <a href="https://2018.igem.org/Safety/Final_Safety_Form?team_id=2615">Click here to check our safety form.</a>
 +
 
 +
 
 
</p>
 
</p>
 
</section>
 
</section>

Revision as of 12:13, 17 October 2018

Team OUC-China: Main
banner

Team member

  Here we are, we are OUC-iGEM. It has been 8 years since we first took part in iGEM in 2011. This time the team which is made up of 8 members pursues a more particular and creative aim. All the members met in May 2017 when we were unfamiliar with each other and knew nothing about synthetic biology. We struck to intergrate the related disciplines and during this time we became not only teammates but also good friends. We come from different colleges and have diverse specialties which ensure that we join our abilities and insights to make our goal clearer as efficiently as possible.

Project design


  Being responsible for our earth and ourselves is our attitude towards every experiment. In our project, all the parts we have utilized are selected from Risk Group 1, none of which are with a Red Flag. What’s more, nonpathogenic bacteria, E.coli DH5 Alpha is employed as the main chassis for our prior design on the DNA sequence, stem-loop and enzyme. As a matter of fact, when using hairpins and csy4 enzyme to tune the expression of genes, it is relatively safe for industrialized applications and scientific research. Based on this, We designed more variants of csy4 enzymes and hairpins. （Click here to see more! ）So we used these to create a toolkit named miniToe family for synthetic biology which can control the expression of the target gene gradient. What’s more, we used the motA gene, one of the genes regulating the motility of E.coli, to verify the feasibility and effectiveness of the toolkit. For the sake of environment, all the chassis organisms will be sterilized before being abandoned to prevent the genes from leaking.

Safe lab work


  We works at a neat lab named “Lab for Microinnovation and Enterprise”, which is a BSL-2 laboratory. Before working in the lab, we are supposed to be trained by the skillful hands, not only for the general lab safety rules, but also for the standard experimental operation avoiding unnecessary damage. No experiment carries no risk to the experimenters, hence we are properly equipped with facilities, such as latex gloves, nitrile gloves, goggles, lab coats to protect us from biotic and abiotic hazards.


Fig.1 Our laboratory and personal safety precautions

  Then for lab safety, a routine inspection is performed twice a day by everyone, not only for ensuring normal operation of our lab like supplying experimental consumables and inspecting the running of instruments, but also keeps it away from non-biological hazards, especially the water and electricity. For high efficiency, responsibility implements to every team member.


Fig.2 Table for routine inspection

  Furthermore, several operational zones are divided scientifically for special experiments like A zone where is weighing the drugs, preparing the medium and sterilizing, B zone where is the recording experimental note and organizing experimental data area, C zone where is a molecular operation area, D zone where is RNA electrophoresis and a polyacrylamide gel electrophoresis area.
  For us, lab safety is a hot topic and we have come up with a practical lab safety manual which normalizes our lovely lab.

Practical lab safety manual


1.Routine inspections must perform between 6:30-8:00pm and 9:30-11:00pm.
2.Lab cleaning must be done twice a week with disinfectant moping the floor for at least 3times.
3.Well prepared experiment plans should be written before you start.
4.Lab coats and gloves must be worn when conducting experiments.
5.Short skirts, shorts, and open shoes must not be worn.
6.If you see a colleague doing something dangerous, point it out to him or her.
7.Lab coats must not be worn outside laboratories and in public areas.
8.Know where safety equipment (eyewash, shower and extinguisher) is located.
9.Food/drink is not allowed in laboratories where chemicals are used stored.
10.Always read the directions before using instruments and read MSDS before handling new chemicals carefully.



Fig.3 Safety operation

Safe shipment


For submitting our parts to igem HQ, we first took the initiative to consult Chinese entry-exit inspection and quarantine laws and regulations about shipping DNA from China to the USA, which is forbidden without tedious red tape. Fortunately, GenScript cooperated with igem HQ forward our part sample to the USA. Ergo, we sealed dried DNA and sent it in the submission kit to GenScript, which clearly labelled as DNA without disguise. Our kit poses no risks and is perfectly safe for shipment.

Safety form


Click here to check our safety form.

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