Difference between revisions of "Team:NUS Singapore-Sci/Parts"

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  <title> NUS Singapore Science: About Us! </title>
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  <div style="font-size: 2.2em; color: #C0392B"> Parts </div>
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<h1>Parts</h1>
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Basic Parts
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h3>Note</h3>
 
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
 
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<table style="width:80%;" class="center_table">
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  <caption style="font-size:13px;"> <strong> Table 1. Basic BioBrick Parts </strong> </caption>
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  <tr>
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    <th>Part Name</th>
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    <th>Component</th>
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    <th>Description</th>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807000">BBa_K2807000</a></td>
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    <td>rAPOBEC1</td>
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    <td>Rat APOBEC1. Used to perform the C-to-U conversion.</td>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807001">BBa_K2807001</a></td>
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    <td>dPspCas13b</td>
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    <td>"Dead" Cas13 from <i>Prevotella sp.</i>. Used to target specific mRNA strands.</td>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807002">BBa_K2807002</a></td>
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    <td>PspCas13b</td>
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    <td>Cas13 from <i>Prevotella sp.</i>. Used to target and cut specific mRNA strands.</td>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807005">BBa_K2807005</a></td>
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    <td>EGFP</td>
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    <td>Constitutively expressed GFP reporter. Used in the Dual Colour Reporter system.</td>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807006">BBa_K2807006</a></td>
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    <td>mCherry</td>
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    <td>Red flurophore protein. Used in the Dual Colour Reporter system.</td>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807007">BBa_K2807007</a></td>
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    <td>T2A</td>
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    <td>Self cleavage peptide. Used in the Dual Colour Reporter system.</td>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807008">BBa_K2807008</a></td>
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    <td>GLB1</td>
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    <td>Human lysosomal acid beta galactosidase. Used in the GLB1 Reporter system.</td>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807009">BBa_K2807009</a></td>
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    <td>EGFP mutant reporter</td>
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    <td>EGFP with a mutated start codon (ATG -> ACG).</td>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807010">BBa_K2807010</a></td>
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    <td>GLB1 CCG mutant</td>
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    <td>Non-functional beta galactosidase with mutation of I389 to P389.</td>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807011">BBa_K2807011</a></td>
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    <td>GLB1 TCT mutant</td>
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    <td>Non-functional beta galactosidase with mutation of F107 to L107.</td>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807017">BBa_K2807017</a></td>
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    <td>EGFP with Kozak sequence</td>
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    <td>Constitutively expressed GFP reporter. Used in the GLB1 Reporter system.</td>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807018">BBa_K2807018</a></td>
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    <td>XTEN</td>
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    <td>Short linker sequence. Used in the Editor system.</td>
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  </tr>
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</table>
  
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Composite Parts
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<h3>Adding parts to the registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<div class="button_link">
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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ADD PARTS
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</a>
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<h3>Inspiration</h3>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
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  <caption style="font-size:13px;"> <strong> Table 2. Composite BioBrick Parts </strong> </caption>
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    <th>Part Name</th>
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    <th>Component</th>
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<h3>What information do I need to start putting my parts on the Registry?</h3>
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    <th>Description</th>
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<p>The information needed to initially create a part on the Registry is:</p>
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  </tr>
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<ul>
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  <tr>
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<li>Part Name</li>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807003">BBa_K2807003</a></td>
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<li>Part type</li>
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    <td>rAPOBEC1-XTEN-dPspCas13b</td>
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<li>Creator</li>
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    <td>The completed Editor system performing C-to-U conversion on targeted mRNA sequences.</td>
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<li>Sequence</li>
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  </tr>
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<li>Short Description (60 characters on what the DNA does)</li>
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  <tr>
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<li>Long Description (Longer description of what the DNA does)</li>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807012">BBa_K2807012</a></td>
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<li>Design considerations</li>
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    <td>EGFP-T2A-mCherry</td>
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</ul>
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    <td>The completed Dual Colour Reporter system. Used to evaluate base-editing efficiency.</td>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807013">BBa_K2807013</a></td>
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    <td>EGFP-T2A-mCherry mutant</td>
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</div>
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    <td>The completed Dual Colour Reporter system with single base mutation in start codon of EGFP. Used to evaluate base-editing efficiency.</td>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807014">BBa_K2807014</a></td>
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    <td>EGFP-GLB1 WT</td>
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    <td>The completed GLB1 Reporter system. Used to evaluate base-editing efficiency.</td>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807015">BBa_K2807015</a></td>
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    <td>EGFP-GLB1 CCG mutant</td>
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    <td>The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.</td>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2807016">BBa_K2807016</a></td>
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    <td>EGFP-GLB1 TCT mutant</td>
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    <td>The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.</td>
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  </tr>
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<h3>Part Table </h3>
 
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
 
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<groupparts>iGEM18 NUS_Singapore-Sci</groupparts>
 
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Latest revision as of 12:29, 17 October 2018

NUS Singapore Science: About Us!

Parts

Basic Parts
Table 1. Basic BioBrick Parts
Part Name Component Description
BBa_K2807000 rAPOBEC1 Rat APOBEC1. Used to perform the C-to-U conversion.
BBa_K2807001 dPspCas13b "Dead" Cas13 from Prevotella sp.. Used to target specific mRNA strands.
BBa_K2807002 PspCas13b Cas13 from Prevotella sp.. Used to target and cut specific mRNA strands.
BBa_K2807005 EGFP Constitutively expressed GFP reporter. Used in the Dual Colour Reporter system.
BBa_K2807006 mCherry Red flurophore protein. Used in the Dual Colour Reporter system.
BBa_K2807007 T2A Self cleavage peptide. Used in the Dual Colour Reporter system.
BBa_K2807008 GLB1 Human lysosomal acid beta galactosidase. Used in the GLB1 Reporter system.
BBa_K2807009 EGFP mutant reporter EGFP with a mutated start codon (ATG -> ACG).
BBa_K2807010 GLB1 CCG mutant Non-functional beta galactosidase with mutation of I389 to P389.
BBa_K2807011 GLB1 TCT mutant Non-functional beta galactosidase with mutation of F107 to L107.
BBa_K2807017 EGFP with Kozak sequence Constitutively expressed GFP reporter. Used in the GLB1 Reporter system.
BBa_K2807018 XTEN Short linker sequence. Used in the Editor system.
Composite Parts
Table 2. Composite BioBrick Parts
Part Name Component Description
BBa_K2807003 rAPOBEC1-XTEN-dPspCas13b The completed Editor system performing C-to-U conversion on targeted mRNA sequences.
BBa_K2807012 EGFP-T2A-mCherry The completed Dual Colour Reporter system. Used to evaluate base-editing efficiency.
BBa_K2807013 EGFP-T2A-mCherry mutant The completed Dual Colour Reporter system with single base mutation in start codon of EGFP. Used to evaluate base-editing efficiency.
BBa_K2807014 EGFP-GLB1 WT The completed GLB1 Reporter system. Used to evaluate base-editing efficiency.
BBa_K2807015 EGFP-GLB1 CCG mutant The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.
BBa_K2807016 EGFP-GLB1 TCT mutant The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.