Difference between revisions of "Team:NUS Singapore-Sci/Parts"

 
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      <a href="" style="text-decoration:inherit; color:black;">Overview</a>
 
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      <a href="" style="text-decoration:inherit; color:black;">Editor System</a>
 
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      <a href="https://2018.igem.org/Team:NUS_Singapore-Sci/Project/Reporter_System" style="text-decoration:inherit; color:black;">Reporter System</a>
 
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      <a href="https://2018.igem.org/Team:NUS_Singapore-Sci/Model" style="text-decoration:inherit; color:black;">Model</a>
 
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      <a class="active" href="https://2018.igem.org/Team:NUS_Singapore-Sci/InterLab" style="text-decoration:inherit; color:black;">Interlab Study</a>
 
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      <a href="" style="text-decoration:inherit; color:black;">Editor System</a>
 
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      <a href="" style="text-decoration:inherit; color:black;">Reporter System</a>
 
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  <a href="" style="text-decoration:inherit; color:white;">Human Practice</a>
 
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  <a href="" style="text-decoration:inherit; color:white;">Parts</a>
 
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  <a href="" style="text-decoration:inherit; color:white;">Collaborations</a>
 
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  <a href="" style="text-decoration:inherit; color:white;">Safety</a>
 
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  <a href="https://2018.igem.org/Team:NUS_Singapore-Sci/Team" style="text-decoration:inherit; color:white;">Team</a>
 
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  <a href="" style="text-decoration:inherit; color:white;">Attributions</a>
 
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  <a href="" style="text-decoration:inherit; color:white;">Medals</a>
 
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<center><title> NUS Singapore Science </title><center>
 
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   <div style="font-size: 2.2em; color: #C0392B"> Parts </div>
 
   <div style="font-size: 2.2em; color: #C0392B"> Parts </div>
 
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</h1>
  
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Basic Parts
 
Basic Parts
 
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<strong> 1) BBa_K2807000 </strong> <br>
 
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Rat APOBEC protein (rAPOBEC)
 
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  <div class="clear extra_space"></div>
 
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<strong> 2) BBa_K2807001 </strong> <br>
 
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Deactivated <i>Prevotella sp</i> CRISPR13b (dPspCas13b)
 
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  <div class="clear extra_space"></div>
 
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<strong> 3) BBa_K2807001=2 </strong> <br>
 
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<i>Prevotella sp</i> CRISPR13b (PspCas13b)
 
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  <div class="clear extra_space"></div>
 
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<strong> 4) BBa_K2807005 </strong> <br>
 
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e Green Fluorescent Protein (eGFP)
 
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  <div class="clear extra_space"></div>
 
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<strong> 5) BBa_K2807006 </strong> <br>
 
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mCherry
 
  
 
<div class="clear extra_space"></div>
 
<div class="clear extra_space"></div>
  
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<strong> 6) BBa_K2807007 </strong> <br>
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<table style="width:80%;" class="center_table">
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T2A
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  <caption style="font-size:13px;"> <strong> Table 1. Basic BioBrick Parts </strong> </caption>
 +
  <tr>
 +
    <th>Part Name</th>
 +
    <th>Component</th>
 +
    <th>Description</th>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807000">BBa_K2807000</a></td>
 +
    <td>rAPOBEC1</td>
 +
    <td>Rat APOBEC1. Used to perform the C-to-U conversion.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807001">BBa_K2807001</a></td>
 +
    <td>dPspCas13b</td>
 +
    <td>"Dead" Cas13 from <i>Prevotella sp.</i>. Used to target specific mRNA strands.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807002">BBa_K2807002</a></td>
 +
    <td>PspCas13b</td>
 +
    <td>Cas13 from <i>Prevotella sp.</i>. Used to target and cut specific mRNA strands.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807005">BBa_K2807005</a></td>
 +
    <td>EGFP</td>
 +
    <td>Constitutively expressed GFP reporter. Used in the Dual Colour Reporter system.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807006">BBa_K2807006</a></td>
 +
    <td>mCherry</td>
 +
    <td>Red flurophore protein. Used in the Dual Colour Reporter system.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807007">BBa_K2807007</a></td>
 +
    <td>T2A</td>
 +
    <td>Self cleavage peptide. Used in the Dual Colour Reporter system.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807008">BBa_K2807008</a></td>
 +
    <td>GLB1</td>
 +
    <td>Human lysosomal acid beta galactosidase. Used in the GLB1 Reporter system.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807009">BBa_K2807009</a></td>
 +
    <td>EGFP mutant reporter</td>
 +
    <td>EGFP with a mutated start codon (ATG -> ACG).</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807010">BBa_K2807010</a></td>
 +
    <td>GLB1 CCG mutant</td>
 +
    <td>Non-functional beta galactosidase with mutation of I389 to P389.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807011">BBa_K2807011</a></td>
 +
    <td>GLB1 TCT mutant</td>
 +
    <td>Non-functional beta galactosidase with mutation of F107 to L107.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807017">BBa_K2807017</a></td>
 +
    <td>EGFP with Kozak sequence</td>
 +
    <td>Constitutively expressed GFP reporter. Used in the GLB1 Reporter system.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807018">BBa_K2807018</a></td>
 +
    <td>XTEN</td>
 +
    <td>Short linker sequence. Used in the Editor system.</td>
 +
  </tr>
 +
</table>
  
 
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<strong> 7) BBa_K2807008 </strong> <br>
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<div class="numberedsection">
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GLB1
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<div class="clear extra_space"></div>
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<strong> 8) BBa_K2807009 </strong> <br>
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eGFP mutant reporter
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<div class="clear extra_space"></div>
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<strong> 9) BBa_K2807010 </strong> <br>
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GLB1 CCG
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<div class="clear extra_space"></div>
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<strong> 10) BBa_K2807011 </strong> <br>
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GLB1 TCT mutant
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<div class="clear extra_space"></div>
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+
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<strong> 10) BBa_K2807017 </strong> <br>
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XTEN
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</div>
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<div class="section">
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Composite Parts
 
Composite Parts
 
</div>
 
</div>
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<div class="text">
 
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<strong> 1) BBa_K2807003 </strong> <br>
 
−
rAPOBEC1-XTEN-dPspCas13b
 
  
 
<div class="clear extra_space"></div>
 
<div class="clear extra_space"></div>
  
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<div class="text">
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<table style="width:80%;" class="center_table">
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<strong> 2) BBa_K2807012 </strong> <br>
+
  <caption style="font-size:13px;"> <strong> Table 2. Composite BioBrick Parts </strong> </caption>
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eGFP-T2A-mCherry
+
  <tr>
 +
    <th>Part Name</th>
 +
    <th>Component</th>
 +
    <th>Description</th>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807003">BBa_K2807003</a></td>
 +
    <td>rAPOBEC1-XTEN-dPspCas13b</td>
 +
    <td>The completed Editor system performing C-to-U conversion on targeted mRNA sequences.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807012">BBa_K2807012</a></td>
 +
    <td>EGFP-T2A-mCherry</td>
 +
    <td>The completed Dual Colour Reporter system. Used to evaluate base-editing efficiency.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807013">BBa_K2807013</a></td>
 +
    <td>EGFP-T2A-mCherry mutant</td>
 +
    <td>The completed Dual Colour Reporter system with single base mutation in start codon of EGFP. Used to evaluate base-editing efficiency.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807014">BBa_K2807014</a></td>
 +
    <td>EGFP-GLB1 WT</td>
 +
    <td>The completed GLB1 Reporter system. Used to evaluate base-editing efficiency.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807015">BBa_K2807015</a></td>
 +
    <td>EGFP-GLB1 CCG mutant</td>
 +
    <td>The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807016">BBa_K2807016</a></td>
 +
    <td>EGFP-GLB1 TCT mutant</td>
 +
    <td>The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.</td>
 +
  </tr>
 +
</table>
  
 
<div class="clear extra_space"></div>
 
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<div class="text">
 
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<strong> 3) BBa_K2807013 </strong> <br>
 
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eGFP-T2A-mCherry mutant
 
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<div class="clear extra_space"></div>
 
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<div class="text">
 
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<strong> 4) BBa_K2807014 </strong> <br>
 
−
eGFP-GLB1 WT
 
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<div class="clear extra_space"></div>
 
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<div class="text">
 
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<strong> 4) BBa_K2807015 </strong> <br>
 
−
eGFP-GLB1 CCG mutant
 
−
 
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<div class="clear extra_space"></div>
 
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<div class="text">
 
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<strong> 5) BBa_K2807016 </strong> <br>
 
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eGFP-GLB1 TCT mutant
 
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Latest revision as of 12:29, 17 October 2018

NUS Singapore Science: About Us!

Parts

Basic Parts
Table 1. Basic BioBrick Parts
Part Name Component Description
BBa_K2807000 rAPOBEC1 Rat APOBEC1. Used to perform the C-to-U conversion.
BBa_K2807001 dPspCas13b "Dead" Cas13 from Prevotella sp.. Used to target specific mRNA strands.
BBa_K2807002 PspCas13b Cas13 from Prevotella sp.. Used to target and cut specific mRNA strands.
BBa_K2807005 EGFP Constitutively expressed GFP reporter. Used in the Dual Colour Reporter system.
BBa_K2807006 mCherry Red flurophore protein. Used in the Dual Colour Reporter system.
BBa_K2807007 T2A Self cleavage peptide. Used in the Dual Colour Reporter system.
BBa_K2807008 GLB1 Human lysosomal acid beta galactosidase. Used in the GLB1 Reporter system.
BBa_K2807009 EGFP mutant reporter EGFP with a mutated start codon (ATG -> ACG).
BBa_K2807010 GLB1 CCG mutant Non-functional beta galactosidase with mutation of I389 to P389.
BBa_K2807011 GLB1 TCT mutant Non-functional beta galactosidase with mutation of F107 to L107.
BBa_K2807017 EGFP with Kozak sequence Constitutively expressed GFP reporter. Used in the GLB1 Reporter system.
BBa_K2807018 XTEN Short linker sequence. Used in the Editor system.
Composite Parts
Table 2. Composite BioBrick Parts
Part Name Component Description
BBa_K2807003 rAPOBEC1-XTEN-dPspCas13b The completed Editor system performing C-to-U conversion on targeted mRNA sequences.
BBa_K2807012 EGFP-T2A-mCherry The completed Dual Colour Reporter system. Used to evaluate base-editing efficiency.
BBa_K2807013 EGFP-T2A-mCherry mutant The completed Dual Colour Reporter system with single base mutation in start codon of EGFP. Used to evaluate base-editing efficiency.
BBa_K2807014 EGFP-GLB1 WT The completed GLB1 Reporter system. Used to evaluate base-editing efficiency.
BBa_K2807015 EGFP-GLB1 CCG mutant The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.
BBa_K2807016 EGFP-GLB1 TCT mutant The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.