Difference between revisions of "Team:NUS Singapore-Sci/Parts"

 
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Line 20: Line 20:
  
 
<table style="width:80%;" class="center_table">
 
<table style="width:80%;" class="center_table">
−
   <caption style="font-size:13px;"> <strong> Table 1. Basic BioBric Parts </strong> </caption>
+
   <caption style="font-size:13px;"> <strong> Table 1. Basic BioBrick Parts </strong> </caption>
 
   <tr>
 
   <tr>
 
     <th>Part Name</th>
 
     <th>Part Name</th>
 
     <th>Component</th>
 
     <th>Component</th>
 
     <th>Description</th>
 
     <th>Description</th>
−
    <th>Submission</th>
 
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
Line 31: Line 30:
 
     <td>rAPOBEC1</td>
 
     <td>rAPOBEC1</td>
 
     <td>Rat APOBEC1. Used to perform the C-to-U conversion.</td>
 
     <td>Rat APOBEC1. Used to perform the C-to-U conversion.</td>
−
    <td>Y</td>
 
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
Line 37: Line 35:
 
     <td>dPspCas13b</td>
 
     <td>dPspCas13b</td>
 
     <td>"Dead" Cas13 from <i>Prevotella sp.</i>. Used to target specific mRNA strands.</td>
 
     <td>"Dead" Cas13 from <i>Prevotella sp.</i>. Used to target specific mRNA strands.</td>
−
    <td>Y</td>
 
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
Line 43: Line 40:
 
     <td>PspCas13b</td>
 
     <td>PspCas13b</td>
 
     <td>Cas13 from <i>Prevotella sp.</i>. Used to target and cut specific mRNA strands.</td>
 
     <td>Cas13 from <i>Prevotella sp.</i>. Used to target and cut specific mRNA strands.</td>
−
    <td>Y</td>
 
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807005">BBa_K2807005</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807005">BBa_K2807005</a></td>
−
     <td>eGFP</td>
+
     <td>EGFP</td>
−
     <td>Consistutively expressed GFP reporter. Used in the Reporter system.</td>
+
     <td>Constitutively expressed GFP reporter. Used in the Dual Colour Reporter system.</td>
−
    <td>N</td>
+
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807006">BBa_K2807006</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807006">BBa_K2807006</a></td>
 
     <td>mCherry</td>
 
     <td>mCherry</td>
−
     <td>Red flurophore protein. Used in the Reporter system.</td>
+
     <td>Red flurophore protein. Used in the Dual Colour Reporter system.</td>
−
    <td>N</td>
+
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807007">BBa_K2807007</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807007">BBa_K2807007</a></td>
 
     <td>T2A</td>
 
     <td>T2A</td>
−
     <td>Self cleavage peptide. Used in the Reporter system.</td>
+
     <td>Self cleavage peptide. Used in the Dual Colour Reporter system.</td>
−
    <td>N</td>
+
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807008">BBa_K2807008</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807008">BBa_K2807008</a></td>
 
     <td>GLB1</td>
 
     <td>GLB1</td>
−
     <td>Human lysosomal acid beta galactosidase. Used in the GLB reporter system.</td>
+
     <td>Human lysosomal acid beta galactosidase. Used in the GLB1 Reporter system.</td>
−
    <td>N</td>
+
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807009">BBa_K2807009</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807009">BBa_K2807009</a></td>
−
     <td>eGFP mutant reporter</td>
+
     <td>EGFP mutant reporter</td>
−
     <td>eGFP with a mutated start codon (ATG -> ACG).</td>
+
     <td>EGFP with a mutated start codon (ATG -> ACG).</td>
−
    <td>N</td>
+
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807010">BBa_K2807010</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807010">BBa_K2807010</a></td>
 
     <td>GLB1 CCG mutant</td>
 
     <td>GLB1 CCG mutant</td>
−
     <td></td>
+
     <td>Non-functional beta galactosidase with mutation of I389 to P389.</td>
−
    <td>N</td>
+
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807011">BBa_K2807011</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807011">BBa_K2807011</a></td>
 
     <td>GLB1 TCT mutant</td>
 
     <td>GLB1 TCT mutant</td>
−
     <td></td>
+
     <td>Non-functional beta galactosidase with mutation of F107 to L107.</td>
−
     <td>N</td>
+
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807017">BBa_K2807017</a></td>
 +
    <td>EGFP with Kozak sequence</td>
 +
    <td>Constitutively expressed GFP reporter. Used in the GLB1 Reporter system.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2807018">BBa_K2807018</a></td>
 +
    <td>XTEN</td>
 +
     <td>Short linker sequence. Used in the Editor system.</td>
 
   </tr>
 
   </tr>
 
</table>
 
</table>
Line 98: Line 97:
  
 
<table style="width:80%;" class="center_table">
 
<table style="width:80%;" class="center_table">
−
   <caption style="font-size:13px;"> <strong> Table 2. Composite BioBric Parts </strong> </caption>
+
   <caption style="font-size:13px;"> <strong> Table 2. Composite BioBrick Parts </strong> </caption>
 
   <tr>
 
   <tr>
 
     <th>Part Name</th>
 
     <th>Part Name</th>
 
     <th>Component</th>
 
     <th>Component</th>
 
     <th>Description</th>
 
     <th>Description</th>
−
    <th>Submission</th>
 
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807003">BBa_K2807003</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807003">BBa_K2807003</a></td>
 
     <td>rAPOBEC1-XTEN-dPspCas13b</td>
 
     <td>rAPOBEC1-XTEN-dPspCas13b</td>
−
     <td>The completed Editor system.</td>
+
     <td>The completed Editor system performing C-to-U conversion on targeted mRNA sequences.</td>
−
    <td>N</td>
+
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807012">BBa_K2807012</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807012">BBa_K2807012</a></td>
−
     <td>eGFP-T2A-mCherry</td>
+
     <td>EGFP-T2A-mCherry</td>
−
     <td>The completed Dual-Reporter system.</td>
+
     <td>The completed Dual Colour Reporter system. Used to evaluate base-editing efficiency.</td>
−
    <td>Y</td>
+
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807013">BBa_K2807013</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807013">BBa_K2807013</a></td>
−
     <td>eGFP-T2A-mCherry mutant</td>
+
     <td>EGFP-T2A-mCherry mutant</td>
−
     <td>The completed Dual-Reporter system, mutated for BioBric competency.</td>
+
     <td>The completed Dual Colour Reporter system with single base mutation in start codon of EGFP. Used to evaluate base-editing efficiency.</td>
−
    <td>Y</td>
+
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807014">BBa_K2807014</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807014">BBa_K2807014</a></td>
−
     <td>eGFP-GLB1 WT</td>
+
     <td>EGFP-GLB1 WT</td>
−
     <td>The completed GLB-Reporter system.</td>
+
     <td>The completed GLB1 Reporter system. Used to evaluate base-editing efficiency.</td>
−
    <td>Y</td>
+
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807015">BBa_K2807015</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807015">BBa_K2807015</a></td>
−
     <td>eGFP-GLB1 CCG mutant</td>
+
     <td>EGFP-GLB1 CCG mutant</td>
−
     <td></td>
+
     <td>The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.</td>
−
    <td>Y</td>
+
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807016">BBa_K2807016</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2807016">BBa_K2807016</a></td>
−
     <td>eGFP-GLB1 TCT mutant</td>
+
     <td>EGFP-GLB1 TCT mutant</td>
−
     <td></td>
+
     <td>The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.</td>
−
    <td>Y</td>
+
 
   </tr>
 
   </tr>
 
</table>
 
</table>

Latest revision as of 12:29, 17 October 2018

NUS Singapore Science: About Us!

Parts

Basic Parts
Table 1. Basic BioBrick Parts
Part Name Component Description
BBa_K2807000 rAPOBEC1 Rat APOBEC1. Used to perform the C-to-U conversion.
BBa_K2807001 dPspCas13b "Dead" Cas13 from Prevotella sp.. Used to target specific mRNA strands.
BBa_K2807002 PspCas13b Cas13 from Prevotella sp.. Used to target and cut specific mRNA strands.
BBa_K2807005 EGFP Constitutively expressed GFP reporter. Used in the Dual Colour Reporter system.
BBa_K2807006 mCherry Red flurophore protein. Used in the Dual Colour Reporter system.
BBa_K2807007 T2A Self cleavage peptide. Used in the Dual Colour Reporter system.
BBa_K2807008 GLB1 Human lysosomal acid beta galactosidase. Used in the GLB1 Reporter system.
BBa_K2807009 EGFP mutant reporter EGFP with a mutated start codon (ATG -> ACG).
BBa_K2807010 GLB1 CCG mutant Non-functional beta galactosidase with mutation of I389 to P389.
BBa_K2807011 GLB1 TCT mutant Non-functional beta galactosidase with mutation of F107 to L107.
BBa_K2807017 EGFP with Kozak sequence Constitutively expressed GFP reporter. Used in the GLB1 Reporter system.
BBa_K2807018 XTEN Short linker sequence. Used in the Editor system.
Composite Parts
Table 2. Composite BioBrick Parts
Part Name Component Description
BBa_K2807003 rAPOBEC1-XTEN-dPspCas13b The completed Editor system performing C-to-U conversion on targeted mRNA sequences.
BBa_K2807012 EGFP-T2A-mCherry The completed Dual Colour Reporter system. Used to evaluate base-editing efficiency.
BBa_K2807013 EGFP-T2A-mCherry mutant The completed Dual Colour Reporter system with single base mutation in start codon of EGFP. Used to evaluate base-editing efficiency.
BBa_K2807014 EGFP-GLB1 WT The completed GLB1 Reporter system. Used to evaluate base-editing efficiency.
BBa_K2807015 EGFP-GLB1 CCG mutant The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.
BBa_K2807016 EGFP-GLB1 TCT mutant The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.