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<table style="width:80%;" class="center_table"> | <table style="width:80%;" class="center_table"> | ||
− | <caption style="font-size:13px;"> <strong> Table 1. Basic | + | <caption style="font-size:13px;"> <strong> Table 1. Basic BioBrick Parts </strong> </caption> |
<tr> | <tr> | ||
<th>Part Name</th> | <th>Part Name</th> | ||
Line 43: | Line 43: | ||
<tr> | <tr> | ||
<td><a href="http://parts.igem.org/Part:BBa_K2807005">BBa_K2807005</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K2807005">BBa_K2807005</a></td> | ||
− | <td> | + | <td>EGFP</td> |
− | <td> | + | <td>Constitutively expressed GFP reporter. Used in the Dual Colour Reporter system.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="http://parts.igem.org/Part:BBa_K2807006">BBa_K2807006</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K2807006">BBa_K2807006</a></td> | ||
<td>mCherry</td> | <td>mCherry</td> | ||
− | <td>Red flurophore protein. Used in the Reporter system.</td> | + | <td>Red flurophore protein. Used in the Dual Colour Reporter system.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="http://parts.igem.org/Part:BBa_K2807007">BBa_K2807007</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K2807007">BBa_K2807007</a></td> | ||
<td>T2A</td> | <td>T2A</td> | ||
− | <td>Self cleavage peptide. Used in the Reporter system.</td> | + | <td>Self cleavage peptide. Used in the Dual Colour Reporter system.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="http://parts.igem.org/Part:BBa_K2807008">BBa_K2807008</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K2807008">BBa_K2807008</a></td> | ||
<td>GLB1</td> | <td>GLB1</td> | ||
− | <td>Human lysosomal acid beta galactosidase. Used in the | + | <td>Human lysosomal acid beta galactosidase. Used in the GLB1 Reporter system.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="http://parts.igem.org/Part:BBa_K2807009">BBa_K2807009</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K2807009">BBa_K2807009</a></td> | ||
− | <td> | + | <td>EGFP mutant reporter</td> |
− | <td> | + | <td>EGFP with a mutated start codon (ATG -> ACG).</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Line 75: | Line 75: | ||
<td>GLB1 TCT mutant</td> | <td>GLB1 TCT mutant</td> | ||
<td>Non-functional beta galactosidase with mutation of F107 to L107.</td> | <td>Non-functional beta galactosidase with mutation of F107 to L107.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2807017">BBa_K2807017</a></td> | ||
+ | <td>EGFP with Kozak sequence</td> | ||
+ | <td>Constitutively expressed GFP reporter. Used in the GLB1 Reporter system.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2807018">BBa_K2807018</a></td> | ||
+ | <td>XTEN</td> | ||
+ | <td>Short linker sequence. Used in the Editor system.</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
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<table style="width:80%;" class="center_table"> | <table style="width:80%;" class="center_table"> | ||
− | <caption style="font-size:13px;"> <strong> Table 2. Composite | + | <caption style="font-size:13px;"> <strong> Table 2. Composite BioBrick Parts </strong> </caption> |
<tr> | <tr> | ||
<th>Part Name</th> | <th>Part Name</th> | ||
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<td><a href="http://parts.igem.org/Part:BBa_K2807003">BBa_K2807003</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K2807003">BBa_K2807003</a></td> | ||
<td>rAPOBEC1-XTEN-dPspCas13b</td> | <td>rAPOBEC1-XTEN-dPspCas13b</td> | ||
− | <td>The completed Editor system.</td> | + | <td>The completed Editor system performing C-to-U conversion on targeted mRNA sequences.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="http://parts.igem.org/Part:BBa_K2807012">BBa_K2807012</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K2807012">BBa_K2807012</a></td> | ||
− | <td> | + | <td>EGFP-T2A-mCherry</td> |
− | <td>The completed Dual | + | <td>The completed Dual Colour Reporter system. Used to evaluate base-editing efficiency.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="http://parts.igem.org/Part:BBa_K2807013">BBa_K2807013</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K2807013">BBa_K2807013</a></td> | ||
− | <td> | + | <td>EGFP-T2A-mCherry mutant</td> |
− | <td>The completed Dual | + | <td>The completed Dual Colour Reporter system with single base mutation in start codon of EGFP. Used to evaluate base-editing efficiency.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="http://parts.igem.org/Part:BBa_K2807014">BBa_K2807014</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K2807014">BBa_K2807014</a></td> | ||
− | <td> | + | <td>EGFP-GLB1 WT</td> |
− | <td>The completed | + | <td>The completed GLB1 Reporter system. Used to evaluate base-editing efficiency.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="http://parts.igem.org/Part:BBa_K2807015">BBa_K2807015</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K2807015">BBa_K2807015</a></td> | ||
− | <td> | + | <td>EGFP-GLB1 CCG mutant</td> |
− | <td></td> | + | <td>The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="http://parts.igem.org/Part:BBa_K2807016">BBa_K2807016</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K2807016">BBa_K2807016</a></td> | ||
− | <td> | + | <td>EGFP-GLB1 TCT mutant</td> |
− | <td></td> | + | <td>The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.</td> |
</tr> | </tr> | ||
</table> | </table> |
Latest revision as of 12:29, 17 October 2018
Parts
Basic Parts
Part Name | Component | Description |
---|---|---|
BBa_K2807000 | rAPOBEC1 | Rat APOBEC1. Used to perform the C-to-U conversion. |
BBa_K2807001 | dPspCas13b | "Dead" Cas13 from Prevotella sp.. Used to target specific mRNA strands. |
BBa_K2807002 | PspCas13b | Cas13 from Prevotella sp.. Used to target and cut specific mRNA strands. |
BBa_K2807005 | EGFP | Constitutively expressed GFP reporter. Used in the Dual Colour Reporter system. |
BBa_K2807006 | mCherry | Red flurophore protein. Used in the Dual Colour Reporter system. |
BBa_K2807007 | T2A | Self cleavage peptide. Used in the Dual Colour Reporter system. |
BBa_K2807008 | GLB1 | Human lysosomal acid beta galactosidase. Used in the GLB1 Reporter system. |
BBa_K2807009 | EGFP mutant reporter | EGFP with a mutated start codon (ATG -> ACG). |
BBa_K2807010 | GLB1 CCG mutant | Non-functional beta galactosidase with mutation of I389 to P389. |
BBa_K2807011 | GLB1 TCT mutant | Non-functional beta galactosidase with mutation of F107 to L107. |
BBa_K2807017 | EGFP with Kozak sequence | Constitutively expressed GFP reporter. Used in the GLB1 Reporter system. |
BBa_K2807018 | XTEN | Short linker sequence. Used in the Editor system. |
Composite Parts
Part Name | Component | Description |
---|---|---|
BBa_K2807003 | rAPOBEC1-XTEN-dPspCas13b | The completed Editor system performing C-to-U conversion on targeted mRNA sequences. |
BBa_K2807012 | EGFP-T2A-mCherry | The completed Dual Colour Reporter system. Used to evaluate base-editing efficiency. |
BBa_K2807013 | EGFP-T2A-mCherry mutant | The completed Dual Colour Reporter system with single base mutation in start codon of EGFP. Used to evaluate base-editing efficiency. |
BBa_K2807014 | EGFP-GLB1 WT | The completed GLB1 Reporter system. Used to evaluate base-editing efficiency. |
BBa_K2807015 | EGFP-GLB1 CCG mutant | The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency. |
BBa_K2807016 | EGFP-GLB1 TCT mutant | The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency. |