Difference between revisions of "Team:HZAU-China/Notebook"
| Line 792: | Line 792: | ||
count, and the result is that the distribution of <i>Salmonella</i> in the tumor cells | count, and the result is that the distribution of <i>Salmonella</i> in the tumor cells | ||
is consistent with the Poisson distribution. | is consistent with the Poisson distribution. | ||
| − | </p> | + | </p> |
| + | <br><br> | ||
<img src="https://static.igem.org/mediawiki/2018/8/86/T--HZAU-China--Notebook-fig1.png" width="100%" alt=""> | <img src="https://static.igem.org/mediawiki/2018/8/86/T--HZAU-China--Notebook-fig1.png" width="100%" alt=""> | ||
| − | <p><b>Figure 1. | + | <p><b>Figure 1. Infection of <i>Salmonella</i> and the result and result of analysis.</b></p> |
<p>The amount of cells and bacteria is obtained through experiments. | <p>The amount of cells and bacteria is obtained through experiments. | ||
</p><br><br> | </p><br><br> | ||
| − | <p> | + | <p>The concentration of bacteria is converted according to MOI and cell density.<br> |
| − | + | We adjusted As to match the curve to the results of the Salmonella infection experiment. When As is equal to 0.0003, the curve simulated by the mathematical model matches the experimental results. | |
| − | + | ||
</p> | </p> | ||
<img src="https://static.igem.org/mediawiki/2018/8/84/T--HZAU-China--Notebook-fig2.png" width="100%" alt=""> | <img src="https://static.igem.org/mediawiki/2018/8/84/T--HZAU-China--Notebook-fig2.png" width="100%" alt=""> | ||
| − | <p><b>Figure 2.</b> </p> | + | <p><b>Figure 2. Parameter fitting of the tumor cells infection experiments.</b> |
| + | <br> | ||
| + | The ‘Nsal’ and ‘Tumor’ is already known before the experiment. When the parameter ‘As’ is 0.0003, the model results fit the experimental results. The red points are corresponding experimental results. </p> | ||
</div> | </div> | ||
Revision as of 12:39, 17 October 2018
1. Verification of Poisson distribution
We infect tumor cells with Salmonella carrying the GFP gene for a period of time and then count, and the result is that the distribution of Salmonella in the tumor cells is consistent with the Poisson distribution.
Figure 1. Infection of Salmonella and the result and result of analysis.
The amount of cells and bacteria is obtained through experiments.
The concentration of bacteria is converted according to MOI and cell density.
We adjusted As to match the curve to the results of the Salmonella infection experiment. When As is equal to 0.0003, the curve simulated by the mathematical model matches the experimental results.
Figure 2. Parameter fitting of the tumor cells infection experiments.
The ‘Nsal’ and ‘Tumor’ is already known before the experiment. When the parameter ‘As’ is 0.0003, the model results fit the experimental results. The red points are corresponding experimental results.
