Difference between revisions of "Team:Vilnius-Lithuania/InterLab"

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  <p>Monodisperse silica microspheres exhibit size and optical characteristics similar to cells, with the additional benefit that the number of particles in a solution is known. Therefore, this measurement allowed us to construct a standard curve which can be used to convert Abs600 measurements to an estimated number of cells.
 
  <p>Monodisperse silica microspheres exhibit size and optical characteristics similar to cells, with the additional benefit that the number of particles in a solution is known. Therefore, this measurement allowed us to construct a standard curve which can be used to convert Abs600 measurements to an estimated number of cells.
 
</p>
 
</p>
  <p> <img src="Fig. 1 https://static.igem.org/mediawiki/2018/3/31/T--Vilnius-Lithuania--1_InterLab.png">  </p>
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  <p> <img src="https://static.igem.org/mediawiki/2018/3/31/T--Vilnius-Lithuania--1_InterLab.png">  </p>
 
       <p><strong>Fig. 1</strong> LUDOX CL-X measurement. Obtained ratiometric conversion factor is 3,419.</p>
 
       <p><strong>Fig. 1</strong> LUDOX CL-X measurement. Obtained ratiometric conversion factor is 3,419.</p>
 
         <p></p>
 
         <p></p>

Revision as of 12:40, 17 October 2018

InterLab

Studying Fluorescence

The goal of this year’s InterLab Study was to identify and minimize the sources of systematic variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of optical density (OD).

Participating in the fifth iGEM InterLab Study was a great opportunity to start this year’s competition as well as acquire some valuable knowledge which we implemented into practice during the project.

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