Difference between revisions of "Team:UPF CRG Barcelona/Composite"
| Line 75: | Line 75: | ||
<div> | <div> | ||
<p class="page-title">COMPOSITE PARTS</p> | <p class="page-title">COMPOSITE PARTS</p> | ||
| − | <p><b>BBa_K2581004: | + | <p><b>BBa_K2581004: t14_pFadBA_32_RFP</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as an intracellular biosensor of fatty acid | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as an intracellular biosensor of fatty acid | ||
uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator | uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator | ||
| Line 82: | Line 82: | ||
fluorescent protein from <i>Discosoma striata</i> (BBa_E1010). </p> | fluorescent protein from <i>Discosoma striata</i> (BBa_E1010). </p> | ||
<center><img src="https://static.igem.org/mediawiki/2018/c/c1/T--UPF_CRG_Barcelona--partpFadBA32.svg" style="width: 45%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/c/c1/T--UPF_CRG_Barcelona--partpFadBA32.svg" style="width: 45%;"></center> | ||
| − | <p><b>BBa_K2581005: | + | <p><b>BBa_K2581005: t14_pFadBA_33_RFP</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as an intracellular biosensor of fatty acid | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as an intracellular biosensor of fatty acid | ||
uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator | uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator | ||
| Line 89: | Line 89: | ||
fluorescent protein from <i>Discosoma striata</i> (BBa_E1010). </p> | fluorescent protein from <i>Discosoma striata</i> (BBa_E1010). </p> | ||
<center><img src="https://static.igem.org/mediawiki/2018/6/6a/T--UPF_CRG_Barcelona--partpFadBA33.svg" style="width: 45%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/6/6a/T--UPF_CRG_Barcelona--partpFadBA33.svg" style="width: 45%;"></center> | ||
| − | <p><b>BBa_K2581006: | + | <p><b>BBa_K2581006: t14_pFadBA_34_RFP</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid | ||
uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator | uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator | ||
| Line 96: | Line 96: | ||
fluorescent protein from <i>Discosoma striata</i> (BBa_E1010). </p> | fluorescent protein from <i>Discosoma striata</i> (BBa_E1010). </p> | ||
<center><img src="https://static.igem.org/mediawiki/2018/b/bc/T--UPF_CRG_Barcelona--partpFadBA34.svg" style="width: 45%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/b/bc/T--UPF_CRG_Barcelona--partpFadBA34.svg" style="width: 45%;"></center> | ||
| − | <p><b>BBa_K2581007: | + | <p><b>BBa_K2581007: t14_pFadBA_34_luxR_T15_32_RFP</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid | ||
uptake. Considering the leakage of the promoter being used a LuxR/pluxR lactone inducible system was coupled | uptake. Considering the leakage of the promoter being used a LuxR/pluxR lactone inducible system was coupled | ||
| Line 109: | Line 109: | ||
<center><img src="https://static.igem.org/mediawiki/2018/3/34/T--UPF_CRG_Barcelona--partlux34.svg" style="width: 50%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/3/34/T--UPF_CRG_Barcelona--partlux34.svg" style="width: 50%;"></center> | ||
| − | <p><b>BBa_K2581008: | + | <p><b>BBa_K2581008: t14_pFadBA_34_luxR_T15_34_RFP</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid | ||
uptake. Considering the leakage of the promoter being used a LuxR/pluxR lactone inducible system was coupled | uptake. Considering the leakage of the promoter being used a LuxR/pluxR lactone inducible system was coupled | ||
| Line 120: | Line 120: | ||
<center><img src="https://static.igem.org/mediawiki/2018/7/76/T--UPF_CRG_Barcelona--partlux3434.svg" style="width: 50%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/7/76/T--UPF_CRG_Barcelona--partlux3434.svg" style="width: 50%;"></center> | ||
| − | <p><b>BBa_K2581009: | + | <p><b>BBa_K2581009: t14_ptet_32_FadD</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist of the expression of the own <i>E. | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist of the expression of the own <i>E. | ||
coli</i> acyl-CoA Synthetase gene, FadD. This gene has an essential role in the fatty acid transportation | coli</i> acyl-CoA Synthetase gene, FadD. This gene has an essential role in the fatty acid transportation | ||
| Line 129: | Line 129: | ||
<center><img src="https://static.igem.org/mediawiki/2018/9/94/T--UPF_CRG_Barcelona--partfadDcomposite.svg" style="width: 45%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/9/94/T--UPF_CRG_Barcelona--partfadDcomposite.svg" style="width: 45%;"></center> | ||
| − | <p><b>BBa_K2581010: | + | <p><b>BBa_K2581010: t14_ptet_32_FadL</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the own <i>E. | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the own <i>E. | ||
coli</i> outer membrane transporter FadL. This gene has an essential role in the fatty acid transportation | coli</i> outer membrane transporter FadL. This gene has an essential role in the fatty acid transportation | ||
| Line 137: | Line 137: | ||
obtained of the own <i>E. coli</i> DH5-alpha strain genome.</p> | obtained of the own <i>E. coli</i> DH5-alpha strain genome.</p> | ||
<center><img src="https://static.igem.org/mediawiki/2018/b/b3/T--UPF_CRG_Barcelona--partfadLcomposite.svg" style="width: 45%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/b/b3/T--UPF_CRG_Barcelona--partfadLcomposite.svg" style="width: 45%;"></center> | ||
| − | <p><b>BBa_K2581019: | + | <p><b>BBa_K2581019: t14_ptet_32_FadE</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the acyl-coA | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the acyl-coA | ||
dehydrogenase (FadE) gene which converts acyl-coA into enoyl-coA.</p> | dehydrogenase (FadE) gene which converts acyl-coA into enoyl-coA.</p> | ||
Revision as of 13:34, 17 October 2018
COMPOSITE PARTS
BBa_K2581004: t14_pFadBA_32_RFP
The UPF_CRG_Barcelona iGEM team 2018 has created this part as an intracellular biosensor of fatty acid uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_k817002), a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581005: t14_pFadBA_33_RFP
The UPF_CRG_Barcelona iGEM team 2018 has created this part as an intracellular biosensor of fatty acid uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_k817002), a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581006: t14_pFadBA_34_RFP
The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), a strong RBS (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581007: t14_pFadBA_34_luxR_T15_32_RFP
The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake. Considering the leakage of the promoter being used a LuxR/pluxR lactone inducible system was coupled in order to reduce high basal activity.
It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_k817002), a strong RBS (BBa_B0034), luxR repressor/activator (BBa_C0062), the double terminator again (BBa_B0015), pLux promoter (BBa_R0062) with a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581008: t14_pFadBA_34_luxR_T15_34_RFP
The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake. Considering the leakage of the promoter being used a LuxR/pluxR lactone inducible system was coupled in order to reduce high basal activity.
It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_k817002), a strong RBS (BBa_B0034), luxR repressor/activator (BBa_C0062) the double terminator again (BBa_B0015), pLux promoter (BBa_R0062) with a weak RBS (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581009: t14_ptet_32_FadD
The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist of the expression of the own E. coli acyl-CoA Synthetase gene, FadD. This gene has an essential role in the fatty acid transportation inside the cell, as well as fatty acid activation. This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a tetR repressible promoter (BBa_R0040), a weak RBS (BBa_B0032) and the the FadD gene (BBa_K258101) obtained of the own E. coli DH5-alpha strain genome.
BBa_K2581010: t14_ptet_32_FadL
The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the own E. coli outer membrane transporter FadL. This gene has an essential role in the fatty acid transportation across the cell membrane via a transportation/acyl-activation mechanism. This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a tetR repressible promoter (BBa_R0040), a weak RBS (BBa_B0032) and the FadL gene (BBa_K2581015) obtained of the own E. coli DH5-alpha strain genome.
BBa_K2581019: t14_ptet_32_FadE
The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the acyl-coA dehydrogenase (FadE) gene which converts acyl-coA into enoyl-coA.
This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a tetR repressible promoter (BBa_R0040), a weak RBS (BBa_B0032) and the FadE gene obtained of the own E. coli DH5-alpha strain genome (BBa_K2581014).
