Difference between revisions of "Team:UPF CRG Barcelona/Improve"
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<a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Description">Project</a> | <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Description">Project</a> | ||
| − | <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/DryLab | + | <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/DryLab">DryLab</a> |
<a class="navbar-item is-active" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Parts">Parts</a> | <a class="navbar-item is-active" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Parts">Parts</a> | ||
<a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Deliverables">Deliverables</a> | <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Deliverables">Deliverables</a> | ||
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<div class="swiper-container" id="sw-container"> | <div class="swiper-container" id="sw-container"> | ||
<div class="swiper-wrapper"> | <div class="swiper-wrapper"> | ||
| − | <a class="swiper-slide swiper-slide-menu" href=" | + | <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Basic"> |
<embed src="https://static.igem.org/mediawiki/2018/2/25/T--UPF_CRG_Barcelona--basicpart.svg" alt=""> | <embed src="https://static.igem.org/mediawiki/2018/2/25/T--UPF_CRG_Barcelona--basicpart.svg" alt=""> | ||
<p>Basic Parts</p> | <p>Basic Parts</p> | ||
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<p>Construction<br>Intermediates</p> | <p>Construction<br>Intermediates</p> | ||
</a> | </a> | ||
| − | <a class="swiper-slide swiper-slide-menu" href=" | + | <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Composite"> |
<embed src="https://static.igem.org/mediawiki/2018/e/ed/T--UPF_CRG_Barcelona--compositepart.svg" alt=""> | <embed src="https://static.igem.org/mediawiki/2018/e/ed/T--UPF_CRG_Barcelona--compositepart.svg" alt=""> | ||
<p>Composite Parts</p> | <p>Composite Parts</p> | ||
</a> | </a> | ||
| − | <a class="swiper-slide swiper-slide-menu" href=" | + | <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Improve"> |
<embed src="https://static.igem.org/mediawiki/2018/9/96/T--UPF_CRG_Barcelona--improvedpart.svg" alt=""> | <embed src="https://static.igem.org/mediawiki/2018/9/96/T--UPF_CRG_Barcelona--improvedpart.svg" alt=""> | ||
<p>Improved Parts</p> | <p>Improved Parts</p> | ||
</a> | </a> | ||
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<p>Parts Registry</p> | <p>Parts Registry</p> | ||
Revision as of 13:54, 17 October 2018
IMPROVED PARTS
BBa_K2581011: T14_pAR_32_RFP or Improved fatty acid acyl-CoA biosensor with medium RBS
The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_K817002). It act as an intracellular biosensor of fatty acid acyl-coA but in this case the high basality activity of the promoter has been reduced.
This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), our improved promoter based on the previous pFadBA DNA sequence (BBa_K2581013), a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581012: T14_pAR_34_RFP or Improved fatty acid acyl-CoA biosensor with strong RBS
The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_k817002). It act as an intracellular biosensor of fatty acid acyl-coA but in this case the high basality activity of the promoter has been reduced.
This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), our improved promoter based on the previous pFadBA DNA sequence (BBa_K2581013), an strong RBS (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
