Difference between revisions of "Team:SDSZ China/Experiment A"

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<header class="align-center">
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        <h2>  chitin deacetylase 1 [Penaeus monodon]    </h2>
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      </header>
 
       <p style="color:black;font-size:35px;">
 
       <p style="color:black;font-size:35px;">
1 atggcaagag taagatgcgg tagttctctt gccctccttg gcattgtgct gccgagcggg<br>
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        1 atggcaagag taagatgcgg tagttctctt gccctccttg gcattgtgct gccgagcggg<br>
 
       61 tcaagaggca ggcagtttca gacacgaccg aggatggagc gaaccttcaa gagggaactg<br>
 
       61 tcaagaggca ggcagtttca gacacgaccg aggatggagc gaaccttcaa gagggaactg<br>
 
      121 tgcaaggata agggcgccgg cgagtggttc cgtctcagcc tcggtgactg tcgcgatgtg<br>
 
      121 tgcaaggata agggcgccgg cgagtggttc cgtctcagcc tcggtgactg tcgcgatgtg<br>

Revision as of 14:03, 17 October 2018

iGem SDSZ_China 2018
...

Obtaining Genes

Both fungal and bacterial acetylases have three functional domains- a deacetylase domain, a chitin binding zone, and a low density lipoprotein receptor region. By using the bioinformatics BLAST technique, a sequence highly conserved with known deacetylase domain sequences was searched in the NCBI soft armor gene database. 

chitin deacetylase 1 [Penaeus monodon]  

1 atggcaagag taagatgcgg tagttctctt gccctccttg gcattgtgct gccgagcggg
       61 tcaagaggca ggcagtttca gacacgaccg aggatggagc gaaccttcaa gagggaactg
      121 tgcaaggata agggcgccgg cgagtggttc cgtctcagcc tcggtgactg tcgcgatgtg
      181 atccagtgta ccgacgccgg tcttcaggcg ctgcgttgcc cccacggcct ggccttcaac
      241 ctggagcagc agacctgcga ctggaaagcc aacgtcaaga actgcgacag gaaggagaag
      301 acgaaggtcg tgaagcctct cttcaacacc gtcgagcccc tttgccagga aaaccagttg
      361 gcgtgcggtg acggcacctg tctggatagg caggtcttct gtgatggaaa agaagattgt
      421 accgatggtt ctgacgagac cgcatgtgac gtgaaaaatg accccaacag cgctcccatc
      481 tgcaacaccg aggactgccg cctccccgat tgctactgct acaacgaccc tagcgagatg
      541 ccccacaaca tgaagccttc cgaagtgcct cagatggtca ccatcacttt cgacgatgcc
      601 atcaacatca acaacatgga cctgtacgag ctcatcttca agcagcgctt caaccccaac
      661 ggctgctcca tcaagtccac cttcttcgtt tctcacaaat acaacaacta caccgccact
      721 caggagatgc accgtctggg tcacgagatc gctgttcact ccatcaccca cgcaaacaac
      781 gagactttct ggtcccacgc aagcgaagac gagtacgagc gcgaaatggg tggtgcccgc
      841 gtcatcatcg aacgcttcgc caacatcacc gaccagtcca tcatcggcat gcgtaatccc
      901 ttcctccgtg tgggcggcaa cagccagttc aggatgatgg agaagaacac cttcctgtac
      961 gactccacca tcactgcccc cctgtcctcc atgcccctgt ggccctacac cctgtactac
     1021 cgcatgcccc acccctgcca tggaaacctc cagaactgcc ccactcgctc cttcgcagtg
     1081 tgggagatgg tcatgaacga gatggaccgt cgcgaggaac ccacttacga ggacggcctc
     1141 cccggatgcc acatggtcga ttcctgcttc gccaccaagc ccgagcccga gcagttctac
     1201 aacttcctgc agaacaactt caaccgccac tacaagtcaa accgcgctcc cttcggtctg
     1261 ttcttccact ctgccttcct gaagaacaac cccgacatcc tggacacctt cctctactgg
     1321 ctggacgaga ccctgaagaa ccagaaggac gtttacttcg tcaccatgac ccaggtcatc
     1381 cagtggatgc aggacccccg ccccgtcggc cagctgaaca actacgaggc ctggaaggag
     1441 aagtgcgtcg tcgacggccc acccttctgc tacggcggca acaactgcga gctggacact
     1501 gacgagctcc ctggccagac cctccacctg tccacctgca tgcggtgccc caacaattat
  1561 ccctggacaa gggacccatt gggcgaggga ttcttctaa