Difference between revisions of "Team:SDSZ China/Experiment A"

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        <h2>PREDICTED: uncharacterized protein LOC108681649 [Hyalella azteca]  </h2>
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1 atgctgatgc atgtagccat catgctcatg ctgctggatc agcacgctca aggttcagag<br>
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       61 cggcaccccc ggcagagcag caacgccacc accacagagc agctctgcag aggccgcgac<br>
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      121 tctgaggagt acttcagact ctctgccggc gacgactgca gagacgttgt tagatgtgac<br>
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      181 cgcagtggac gctctggccc cagccgcctg gccgcagtgc ggtgccccaa cggcctcgcc<br>
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      241 ttcgacgtcg acagacaagt ctgtgactgg aagaccaaag tccgcaactg cgacagactt<br>
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      301 gagagaccgc ggaagattaa gccgctgctg gtgacggagg agccgctgtg ctcggggacg<br>
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      361 gacctggcgt gcggcagcgg cgtgtgtgtc gccaaggagc tcttctgtga tggcaaacct<br>
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      421 gactgtgatg acggttccga cgagaacacg tgtggtgtgg aagaagatcc aaaccgagcc<br>
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      481 caggtgtgcg acaaaagtca atgcgttttg ccagaatgtt tctgttccgt ggacggcact<br>
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      541 agaattcctg gtgatatcaa cccaaaacaa acaccgcaaa tgatcaccat cacattttca<br>
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      601 ggagcaatca accttgataa tgtggatttg tacgatgaca tcttcaacgg ggaaagaaag<br>
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      661 aatcccaacg gttgtcaagt gaaagcaacg ttcttcacgt cgcacaagta caccaattac<br>
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      721 tccgcagttc aagaactaca caggaaaggc catgagatcg ctgtgttctc gatctcgaac<br>
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      781 aaagaaagtc gagactattg gtcccatgga acatacgacg actggcttgc tgaaatggct<br>
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      841 ggggccagac tcatcgtcga acgtttcgcc aatatcacag ataattccat cgtcgggtta<br>
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      901 cgagcgccct acctcagggt tggaggaaac gctcagttcg acatgatgaa cgatcagttc<br>
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      961 ttcttctacg acgcttcgat tacagcgcca ttggggaaac ttccgctatg gccgtacaca<br>
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     1021 ttgtacttca ggatgcctca caaatgtcac gggaacggcc aaaattgtcc atcacgctcc<br>
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     1081 caccccgtct gggaaatggt catgaacgaa atggacagaa gagacgaccc agagtttgac<br>
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     1141 gaaggactat ctgggtgtca ttacgtcgac tcctgcacca acatccggac tccaaagcaa<br>
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     1201 tttgcccatt tcctcgagca caacttcaga cgacactaca acacaaaccg tgcaccactc<br>
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     1261 ggcttgcatt tccacgcttc ttggctaaaa ggaaacaaaa attttaaaaa ggaactcatc<br>
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     1321 aatttcattc agtcaaaatc gggcaaccct gacgtgtatt tcgtgaccat gcttcaagtg<br>
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     1381 attcaatgga tgcaagcacc gaccgaagtg gcaggacttc gcgatttccc agaatggaag<br>
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     1441 gagaaatgcg acgtgcaagg tctgccattt tgctcattac caaacacttg cccggtgagg<br>
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     1501 acacgagaga ttcctgacga gactttgagt ttgttcacct gcatggactg cccccgcaac<br>
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     1561 tacccctggc tgctcgaccc taccggggat ggcgtggaaa ttatataa<br>
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Revision as of 14:10, 17 October 2018

iGem SDSZ_China 2018
...

Obtaining Genes

Both fungal and bacterial acetylases have three functional domains- a deacetylase domain, a chitin binding zone, and a low density lipoprotein receptor region. By using the bioinformatics BLAST technique, a sequence highly conserved with known deacetylase domain sequences was searched in the NCBI soft armor gene database. 

chitin deacetylase 1 [Penaeus monodon]  

1 atggcaagag taagatgcgg tagttctctt gccctccttg gcattgtgct gccgagcggg
       61 tcaagaggca ggcagtttca gacacgaccg aggatggagc gaaccttcaa gagggaactg
      121 tgcaaggata agggcgccgg cgagtggttc cgtctcagcc tcggtgactg tcgcgatgtg
      181 atccagtgta ccgacgccgg tcttcaggcg ctgcgttgcc cccacggcct ggccttcaac
      241 ctggagcagc agacctgcga ctggaaagcc aacgtcaaga actgcgacag gaaggagaag
      301 acgaaggtcg tgaagcctct cttcaacacc gtcgagcccc tttgccagga aaaccagttg
      361 gcgtgcggtg acggcacctg tctggatagg caggtcttct gtgatggaaa agaagattgt
      421 accgatggtt ctgacgagac cgcatgtgac gtgaaaaatg accccaacag cgctcccatc
      481 tgcaacaccg aggactgccg cctccccgat tgctactgct acaacgaccc tagcgagatg
      541 ccccacaaca tgaagccttc cgaagtgcct cagatggtca ccatcacttt cgacgatgcc
      601 atcaacatca acaacatgga cctgtacgag ctcatcttca agcagcgctt caaccccaac
      661 ggctgctcca tcaagtccac cttcttcgtt tctcacaaat acaacaacta caccgccact
      721 caggagatgc accgtctggg tcacgagatc gctgttcact ccatcaccca cgcaaacaac
      781 gagactttct ggtcccacgc aagcgaagac gagtacgagc gcgaaatggg tggtgcccgc
      841 gtcatcatcg aacgcttcgc caacatcacc gaccagtcca tcatcggcat gcgtaatccc
      901 ttcctccgtg tgggcggcaa cagccagttc aggatgatgg agaagaacac cttcctgtac
      961 gactccacca tcactgcccc cctgtcctcc atgcccctgt ggccctacac cctgtactac
     1021 cgcatgcccc acccctgcca tggaaacctc cagaactgcc ccactcgctc cttcgcagtg
     1081 tgggagatgg tcatgaacga gatggaccgt cgcgaggaac ccacttacga ggacggcctc
     1141 cccggatgcc acatggtcga ttcctgcttc gccaccaagc ccgagcccga gcagttctac
     1201 aacttcctgc agaacaactt caaccgccac tacaagtcaa accgcgctcc cttcggtctg
     1261 ttcttccact ctgccttcct gaagaacaac cccgacatcc tggacacctt cctctactgg
     1321 ctggacgaga ccctgaagaa ccagaaggac gtttacttcg tcaccatgac ccaggtcatc
     1381 cagtggatgc aggacccccg ccccgtcggc cagctgaaca actacgaggc ctggaaggag
     1441 aagtgcgtcg tcgacggccc acccttctgc tacggcggca acaactgcga gctggacact
     1501 gacgagctcc ctggccagac cctccacctg tccacctgca tgcggtgccc caacaattat
  1561 ccctggacaa gggacccatt gggcgaggga ttcttctaa

PREDICTED: uncharacterized protein LOC108681649 [Hyalella azteca]  

1 atgctgatgc atgtagccat catgctcatg ctgctggatc agcacgctca aggttcagag
       61 cggcaccccc ggcagagcag caacgccacc accacagagc agctctgcag aggccgcgac
      121 tctgaggagt acttcagact ctctgccggc gacgactgca gagacgttgt tagatgtgac
      181 cgcagtggac gctctggccc cagccgcctg gccgcagtgc ggtgccccaa cggcctcgcc
      241 ttcgacgtcg acagacaagt ctgtgactgg aagaccaaag tccgcaactg cgacagactt
      301 gagagaccgc ggaagattaa gccgctgctg gtgacggagg agccgctgtg ctcggggacg
      361 gacctggcgt gcggcagcgg cgtgtgtgtc gccaaggagc tcttctgtga tggcaaacct
      421 gactgtgatg acggttccga cgagaacacg tgtggtgtgg aagaagatcc aaaccgagcc
      481 caggtgtgcg acaaaagtca atgcgttttg ccagaatgtt tctgttccgt ggacggcact
      541 agaattcctg gtgatatcaa cccaaaacaa acaccgcaaa tgatcaccat cacattttca
      601 ggagcaatca accttgataa tgtggatttg tacgatgaca tcttcaacgg ggaaagaaag
      661 aatcccaacg gttgtcaagt gaaagcaacg ttcttcacgt cgcacaagta caccaattac
      721 tccgcagttc aagaactaca caggaaaggc catgagatcg ctgtgttctc gatctcgaac
      781 aaagaaagtc gagactattg gtcccatgga acatacgacg actggcttgc tgaaatggct
      841 ggggccagac tcatcgtcga acgtttcgcc aatatcacag ataattccat cgtcgggtta
      901 cgagcgccct acctcagggt tggaggaaac gctcagttcg acatgatgaa cgatcagttc
      961 ttcttctacg acgcttcgat tacagcgcca ttggggaaac ttccgctatg gccgtacaca
     1021 ttgtacttca ggatgcctca caaatgtcac gggaacggcc aaaattgtcc atcacgctcc
     1081 caccccgtct gggaaatggt catgaacgaa atggacagaa gagacgaccc agagtttgac
     1141 gaaggactat ctgggtgtca ttacgtcgac tcctgcacca acatccggac tccaaagcaa
     1201 tttgcccatt tcctcgagca caacttcaga cgacactaca acacaaaccg tgcaccactc
     1261 ggcttgcatt tccacgcttc ttggctaaaa ggaaacaaaa attttaaaaa ggaactcatc
     1321 aatttcattc agtcaaaatc gggcaaccct gacgtgtatt tcgtgaccat gcttcaagtg
     1381 attcaatgga tgcaagcacc gaccgaagtg gcaggacttc gcgatttccc agaatggaag
     1441 gagaaatgcg acgtgcaagg tctgccattt tgctcattac caaacacttg cccggtgagg
     1501 acacgagaga ttcctgacga gactttgagt ttgttcacct gcatggactg cccccgcaac
     1561 tacccctggc tgctcgaccc taccggggat ggcgtggaaa ttatataa

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