Difference between revisions of "Team:NEU China A"

 
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<a href="https://2018.igem.org/Team:NEU_China_A/pages">建立页面</a>
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<h2> Project Description </h2>
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<h3>Engineered probiotics alleviate the inflammatory bowel disease and prevent colorectal cancer</h3>
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<p>Due to the popularity of fast food and accelerated urbanization nowadays, the number of patients with inflammatory bowel disease(IBD) is rising in Asia. In addition, patients with IBD have an increased risk of developing colorectal cancer(CRC). Therefore, NEU China A aim to design a biological system against IBD and potential CRC by using engineered probiotics this year. To put out the fire in the gut, E. coli Nissle 1917 is selected as our firemen. We empower it with an anti-inflammatory device, which includes a sensor to detect the inflammatory signal, an amplifier to enhance the work ability through positive-feedback loop and an effector to secret anti-inflammatory part. Besides, we should watch out the bad guys such as cancer cells or invasive bacterial. So, we choose Saccharomyces boulardii to be our policemen. It is armed with a constitutively expressed myrosinase to turn the glucosinolates, a natural component of cruciferous vegetables, to sulphoraphane which is an organic molecule with known anticancer activity. We also equip our E. coli with this module since the sulphoraphane can inhibit the oxidative stress. In other hand, to restrict the bacterial invasion, the yeast is made to produce Lypd8, a protein represses the move of bacterial by binding to the flagella. Moreover, we decide to use a cold shock kill switch to avoid the escape of our chassis to the environment and more supporting modules will be added if necessary. We hope these modules will incorporate into a functional system eventually. Integrating probiotic therapy with synthetic biology, we envision that the collaboration between the firemen and the policemen will greatly help us to overcome the tough situation in the patient’s gut.
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<h1> Welcome to iGEM 2018! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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        <br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
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<li> <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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        <h2 class="header center">NEU-China-A</h3>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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        <h3 class="header center">Overview</h3>
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<h3> Uploading pictures and files </h3>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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        The goal of NEU_China_A this year is to design a biological system aiming to alleviate intestinal inflammatory
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        diseases and prevent potential colorectal cancer. We chose E. coli Nissle 1917 as our chassis, a probiotic that
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        is safe for humans. On the one hand, when it senses an inflammatory signal in the intestine, it releases an
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        anti-inflammatory drug (interleukin-10) to put out the fire in the intestines. On the other hand, it can
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        release myrosinase to convert the glucosinolate contained in cruciferous vegetables into sulforaphane. The
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        sulforaphane can both alleviate inflammation in the intestine and prevent colorectal cancer induced by chronic
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        inflammation.
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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            <h3 class="header center">Intestinal homeostasis</h3>
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                There are a lot of microorganisms living in the intestines. The intestinal villi and the underlying
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                tissueshave
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                the largest immune cell population in the human body. Harmonious coexistence between the
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                intestinalflora and
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                the immune cells is critical to human health. Consequently, the intestine has evolved
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                severalmechanisms.
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        <img class="responsive-img" src="https://static.igem.org/mediawiki/2018/1/1a/T--NEU_China_A--fig1.png" alt="Intestinal homeostasis" />
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                    <p>In the crypt, the goblet cells secrete mucus covering the surface of the intestinal villi and
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                        Paneth cells
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                        secrete antibacterial peptides to inhibit bacterial invasion. In addition, the basal stem cells
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                        continue to
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                        proliferate and differentiate to renew the intestinal epithelium.
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                    <p>Peyer’s patch contains a large amount of lymphoid tissues, which is the key to regulating the
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                        immune
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                        system's response to pathogens, and is tolerant to harmless microorganisms and food. Dendritic
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                        Peyer’s patch can extend dendrites among epithelial cells, collecting antigen and decomposing
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                        it, by which
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                        to activate the tolerability of lymphoid cells.</p>
  
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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                    <p>Regulatory T cells secrete IL-10 in the lamina propria, inhibit the immune cells of the lamina
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                        epithelial layers, preventing vigorous immunoreaction along with unnecessary inflammation. Once
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                        imbalance of homeostasis in the immune system occurs, it can cause intestinal disease. When
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                        this condition
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                        persists without intervention, it can lead to inflammatory bowel disease.</p>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Latest revision as of 14:15, 17 October 2018

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NEU-China-A

Overview

Overview

The goal of NEU_China_A this year is to design a biological system aiming to alleviate intestinal inflammatory diseases and prevent potential colorectal cancer. We chose E. coli Nissle 1917 as our chassis, a probiotic that is safe for humans. On the one hand, when it senses an inflammatory signal in the intestine, it releases an anti-inflammatory drug (interleukin-10) to put out the fire in the intestines. On the other hand, it can release myrosinase to convert the glucosinolate contained in cruciferous vegetables into sulforaphane. The sulforaphane can both alleviate inflammation in the intestine and prevent colorectal cancer induced by chronic inflammation.




Intestinal homeostasis


There are a lot of microorganisms living in the intestines. The intestinal villi and the underlying tissueshave the largest immune cell population in the human body. Harmonious coexistence between the intestinalflora and the immune cells is critical to human health. Consequently, the intestine has evolved severalmechanisms.


Intestinal homeostasis

In the crypt, the goblet cells secrete mucus covering the surface of the intestinal villi and Paneth cells secrete antibacterial peptides to inhibit bacterial invasion. In addition, the basal stem cells continue to proliferate and differentiate to renew the intestinal epithelium.

Peyer’s patch contains a large amount of lymphoid tissues, which is the key to regulating the immune system's response to pathogens, and is tolerant to harmless microorganisms and food. Dendritic cells in the Peyer’s patch can extend dendrites among epithelial cells, collecting antigen and decomposing it, by which to activate the tolerability of lymphoid cells.

Regulatory T cells secrete IL-10 in the lamina propria, inhibit the immune cells of the lamina propria and epithelial layers, preventing vigorous immunoreaction along with unnecessary inflammation. Once the imbalance of homeostasis in the immune system occurs, it can cause intestinal disease. When this condition persists without intervention, it can lead to inflammatory bowel disease.