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| − | <h1>Parts</h1>
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| − | <p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code><groupparts></code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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| − | <p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p> | + | <h1 class="pagetitle">Parts</h1> |
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| − | <h3>Note</h3>
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| − | <p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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| − | <h3>Adding parts to the registry</h3>
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| − | <p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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| − | <p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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| − | <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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| − | ADD PARTS
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| − | <h3>Inspiration</h3>
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| − | <p>We have a created a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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| − | <p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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| − | <li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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| − | <li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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| − | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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| − | <h3>What information do I need to start putting my parts on the Registry?</h3>
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| − | <p>The information needed to initially create a part on the Registry is:</p>
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| − | <li>Part Name</li>
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| − | <li>Part type</li>
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| − | <li>Creator</li>
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| − | <li>Sequence</li>
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| − | <li>Short Description (60 characters on what the DNA does)</li>
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| − | <li>Long Description (Longer description of what the DNA does)</li>
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| − | <li>Design considerations</li>
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| − | <p>
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| − | We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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| − | <h3>Part Table </h3>
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| − | <p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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| − | <groupparts>iGEM18 NDC-HighRiverAB</groupparts>
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| | + | <p>This year our team created two new parts: a basic part and a composite part. Our basic part is EstA, our esterase gene. Our composite part drives expression of this part with a pLac promoter from the registry <a href="http://parts.igem.org/Part:BBa_R0011">(Part:BBa_B0011)</a>. We submitted our composite part to the registry and were able to characterize this part's functionality. In order to reach our goal of breaking down fats, which are a major component of buildups in wastewater systems, we selected the following biobrick parts for our plasmid with the goal of producing esterase:</p> |
| | + | <p> - First, we researched an esterase-producing gene that could be expressed in E.coli. We decided to use the EstA gene, which is found in Pseudomonas aeruginosa.<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2694001">(BBa_K2694001)</a></p> |
| | + | <p> - For our promoter, we decided to use pLAC, which is IPTG-inducible and is naturally found in E.coli.<a href="http://parts.igem.org/Part:BBa_R0011">(Part:BBa_B0011)</a></p> |
| | + | <p> - The RBS we used is B0034. <a href="http://parts.igem.org/Part:BBa_B0034">(Part:BBa_B0034)</a></p> |
| | + | <p> - Finally, we chose the double terminator B0015, which is the most common terminator and is known to be reliable. <a href="http://parts.igem.org/Part:BBa_B0015">(Part:BBa_B0015)</a></p> |
| | + | <p> - After we had chosen all of our biobrick parts, we combined them into the plasmid <a href="http://parts.igem.org/Part:BBa_K2694000">BBa_K2694000</a></p> |
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This year our team created two new parts: a basic part and a composite part. Our basic part is EstA, our esterase gene. Our composite part drives expression of this part with a pLac promoter from the registry (Part:BBa_B0011). We submitted our composite part to the registry and were able to characterize this part's functionality. In order to reach our goal of breaking down fats, which are a major component of buildups in wastewater systems, we selected the following biobrick parts for our plasmid with the goal of producing esterase:
- First, we researched an esterase-producing gene that could be expressed in E.coli. We decided to use the EstA gene, which is found in Pseudomonas aeruginosa.(BBa_K2694001)
- For our promoter, we decided to use pLAC, which is IPTG-inducible and is naturally found in E.coli.(Part:BBa_B0011)
- Finally, we chose the double terminator B0015, which is the most common terminator and is known to be reliable. (Part:BBa_B0015)
- After we had chosen all of our biobrick parts, we combined them into the plasmid BBa_K2694000
