Difference between revisions of "Team:NDC-HighRiverAB/Parts"
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<p>This year our team created two new parts: a basic part and a composite part. Our basic part is EstA, our esterase gene. Our composite part drives expression of this part with a pLac promoter from the registry <a href="http://parts.igem.org/Part:BBa_R0011">(Part:BBa_B0011)</a>. We submitted our composite part to the registry and were able to characterize this part's functionality. In order to reach our goal of breaking down fats, which are a major component of buildups in wastewater systems, we selected the following biobrick parts for our plasmid with the goal of producing esterase:</p> | <p>This year our team created two new parts: a basic part and a composite part. Our basic part is EstA, our esterase gene. Our composite part drives expression of this part with a pLac promoter from the registry <a href="http://parts.igem.org/Part:BBa_R0011">(Part:BBa_B0011)</a>. We submitted our composite part to the registry and were able to characterize this part's functionality. In order to reach our goal of breaking down fats, which are a major component of buildups in wastewater systems, we selected the following biobrick parts for our plasmid with the goal of producing esterase:</p> | ||
| − | < | + | <p> - First, we researched an esterase-producing gene that could be expressed in E.coli. We decided to use the EstA gene, which is found in Pseudomonas aeruginosa.<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2694001">(BBa_K2694001)</a></p> |
| − | < | + | <p> - For our promoter, we decided to use pLAC, which is IPTG-inducible and is naturally found in E.coli.<a href="http://parts.igem.org/Part:BBa_R0011">(Part:BBa_B0011)</a></p> |
| − | < | + | <p> - The RBS we used is B0034. <a href="http://parts.igem.org/Part:BBa_B0034">(Part:BBa_B0034)</a></p> |
| − | < | + | <p> - Finally, we chose the double terminator B0015, which is the most common terminator and is known to be reliable. <a href="http://parts.igem.org/Part:BBa_B0015">(Part:BBa_B0015)</a></p> |
| − | < | + | <p> - After we had chosen all of our biobrick parts, we combined them into the plasmid <a href="http://parts.igem.org/Part:BBa_K2694000">BBa_K2694000</a></p> |
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Latest revision as of 14:39, 17 October 2018
Parts
This year our team created two new parts: a basic part and a composite part. Our basic part is EstA, our esterase gene. Our composite part drives expression of this part with a pLac promoter from the registry (Part:BBa_B0011). We submitted our composite part to the registry and were able to characterize this part's functionality. In order to reach our goal of breaking down fats, which are a major component of buildups in wastewater systems, we selected the following biobrick parts for our plasmid with the goal of producing esterase:
- First, we researched an esterase-producing gene that could be expressed in E.coli. We decided to use the EstA gene, which is found in Pseudomonas aeruginosa.(BBa_K2694001)
- For our promoter, we decided to use pLAC, which is IPTG-inducible and is naturally found in E.coli.(Part:BBa_B0011)
- The RBS we used is B0034. (Part:BBa_B0034)
- Finally, we chose the double terminator B0015, which is the most common terminator and is known to be reliable. (Part:BBa_B0015)
- After we had chosen all of our biobrick parts, we combined them into the plasmid BBa_K2694000