Difference between revisions of "Team:AHUT China/Notebook"

 
(62 intermediate revisions by 4 users not shown)
Line 1: Line 1:
 
{{:Team:AHUT_China/Template/CSS_RESET}}
 
{{:Team:AHUT_China/Template/CSS_RESET}}
 +
 
<html lang="en">
 
<html lang="en">
 
     <head>
 
     <head>
Line 21: Line 22:
 
      
 
      
 
         <!--================Header Area =================-->
 
         <!--================Header Area =================-->
−
            <header class="header_area">
+
            <header class="header_area">
 
             <div class="container">
 
             <div class="container">
 
                 <nav class="navbar navbar-expand-lg navbar-light">
 
                 <nav class="navbar navbar-expand-lg navbar-light">
 
                     <!-- Brand and toggle get grouped for better mobile display -->
 
                     <!-- Brand and toggle get grouped for better mobile display -->
−
                     <a class="navbar-brand logo_h" href="https://2018.igem.org/Team:AHUT_China/home"><img src="https://static.igem.org/mediawiki/2018/e/ec/T--AHUT_China--_logo.jpg" height="100" width="80" alt=""></a>
+
                     <a class="navbar-brand logo_h" href="https://2018.igem.org/Team:AHUT_China/home"><img src="https://static.igem.org/mediawiki/2018/b/b0/T--AHUT_China--_newlogo.jpg" height="110" width="80" alt=""></a>
 
                     <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#navbarSupportedContent" aria-controls="navbarSupportedContent" aria-expanded="false" aria-label="Toggle navigation">
 
                     <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#navbarSupportedContent" aria-controls="navbarSupportedContent" aria-expanded="false" aria-label="Toggle navigation">
 
                         <span class="icon-bar"></span>
 
                         <span class="icon-bar"></span>
Line 34: Line 35:
 
                     <div class="collapse navbar-collapse offset" id="navbarSupportedContent">
 
                     <div class="collapse navbar-collapse offset" id="navbarSupportedContent">
 
                         <ul class="nav navbar-nav menu_nav ml-auto">
 
                         <ul class="nav navbar-nav menu_nav ml-auto">
−
                             <li class="nav-item active"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/home">Home</a></li>  
+
                             <li class="nav-item "><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/home">Home</a></li>  
 
                             <li class="nav-item submenu dropdown">
 
                             <li class="nav-item submenu dropdown">
 
                                 <a href="#" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">TEAM</a>
 
                                 <a href="#" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">TEAM</a>
Line 41: Line 42:
 
">Team Members</a></li>
 
">Team Members</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Attributions">Attribution</a>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Attributions">Attribution</a>
−
<li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Sponsoring">Sponsoring</a></li>
+
 
                                 </ul>
 
                                 </ul>
 
                             </li>  
 
                             </li>  
 
                             <li class="nav-item submenu dropdown">
 
                             <li class="nav-item submenu dropdown">
−
                                 <a href="#" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">PROJECT</a>
+
                                 <a href="#" style="color:#52C5FD" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">PROJECT</a>
 
                                 <ul class="dropdown-menu">
 
                                 <ul class="dropdown-menu">
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Overview">Overview</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Overview">Overview</a></li>
 +
                                    <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Background">Background</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/protocol">Protocol</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/protocol">Protocol</a></li>
 
                                     <li class="nav-item"><a class="nav-link"
 
                                     <li class="nav-item"><a class="nav-link"
−
href="https://2018.igem.org/Team:AHUT_China/design">Design</a></li>
+
href="https://2018.igem.org/Team:AHUT_China/design">Design</a></li>                                                    
−
                                    <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Background">Background</a></li>
+
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Demonstrate">Demonstrate</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Demonstrate">Demonstrate</a></li>
 
    <li class="nav-item"><a class="nav-link" href="
 
    <li class="nav-item"><a class="nav-link" href="
 
https://2018.igem.org/Team:AHUT_China/InterLab">Interlab</a></li>
 
https://2018.igem.org/Team:AHUT_China/InterLab">Interlab</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Notebook">Notebook</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Notebook">Notebook</a></li>
 +
                                    <li class="nav-item"><a class="nav-link"
 +
href="https://2018.igem.org/Team:AHUT_China/Result">Result</a></li>
 
                                   </li>
 
                                   </li>
 
                                 </ul>
 
                                 </ul>
Line 74: Line 77:
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/HP_FOR_SILVER">HP for Silver</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/HP_FOR_SILVER">HP for Silver</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/INTEGRATED_FOR_GOLD">Integrated HP for gold</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/INTEGRATED_FOR_GOLD">Integrated HP for gold</a></li>
 +
                                    <li class="nav-item"><a class="nav-link" href="
 +
https://2018.igem.org/Team:AHUT_China/Public_Engagement">Public Engagement</a></li>
 
                                 </ul>
 
                                 </ul>
 
                             </li>  
 
                             </li>  
Line 82: Line 87:
 
                 </nav>
 
                 </nav>
 
             </div>
 
             </div>
−
         
+
     
 
         </header>
 
         </header>
−
       
+
  <?<div align="center"></div>>       
−
       
+
−
       
+
 
         <!--================Header Area =================-->
 
         <!--================Header Area =================-->
 
        
 
        
Line 93: Line 96:
 
         <!--================Breadcrumb Area =================-->
 
         <!--================Breadcrumb Area =================-->
 
         <!--================ Latest Blog Area  =================-->
 
         <!--================ Latest Blog Area  =================-->
−
         <section class="latest_blog_area section_gap">
+
 
−
            <div class="container">
+
         <!--================ start footer Area  =================-->
−
              <div class="section_title ">
+
 
−
                <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;"> 8.29</strong></h2>
+
      <div align="center">    
−
                <hr>
+
<br><br><br><br><br><br>
−
                <nbsp><nbsp>
+
<p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 28px; color: rgba(248,6,6,1);center" >
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 14px;"> <span style="font-size: 16px">&nbsp;&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">&nbsp;Pick</span> Clon  </span></p>
+
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The details of the experimental record are as follows:<br></p>
−
<br><br>
+
<iframe src="https://static.igem.org/mediawiki/2018/9/9b/T--AHUT_China--Notebook.pdf" width="1000" height="800"></iframe>
−
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/0/02/T--AHUT_China--829.png" width="450" height="77" alt=""/></div><br>
+
</div>
−
    </div>
+
−
<div class="container">
+
−
              <div class="section_title ">
+
−
                  <p style="text-align: center; font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 36px;"><strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400; color: #000000;">8.30</strong></p>
+
−
                </h2>
+
−
                <hr>
+
−
                 
+
−
                  <nbsp><nbsp>
+
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px; ">&nbsp;&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">Pilot </span> Expression of Protein<br>&nbsp;&nbsp;&nbsp;“IPTG+ 4h”“IPTG- 4h”</p> 
+
−
<p>&nbsp;</p> <p>&nbsp;</p>             
+
−
 
+
−
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/4/44/T--AHUT_China--830.png" width="420" height="77" alt=""/></div><p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px;">&nbsp;&nbsp;&nbsp;After the rocker culture is 4 H, add IPTG(final concentration is 0.5 mm) and then the rocker culture is 4 hours. Each of the two tubes absorbs 200 μL of bacterial fluid, and in the frozen centrifuge, it is 4 °C and 12000 rpm centrifuge. 10 minutes, go to the clear, It is resuspended with 100 μL 1x SDS Loading buffer, then heated in a water bath pot at 100 °C for 10 minutes and stored in a -20 °C refrigerator.</p>
+
−
          </div>
+
−
                        <div class="container">
+
−
              <div class="section_title ">
+
−
                <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;"> 8.31</strong></h2>
+
−
                <hr>
+
−
                <nbsp><nbsp>
+
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 16px;">&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">&nbsp;Protein</span> extraction <br>&nbsp;&nbsp;&nbsp;CA2L203K</p> 
+
−
<p>&nbsp;</p> <p>&nbsp;</p> 
+
−
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/2/2d/T--AHUT_China--831.11.png" width="761" height="76" alt=""/></div><br>
+
−
    </div>
+
−
        <div class="container">
+
−
              <div class="section_title ">
+
−
                <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;"> 8.29</strong></h2>
+
−
                <hr>
+
−
                <nbsp><nbsp>
+
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 14px;"> <span style="font-size: 16px">&nbsp;&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">&nbsp;Pick</span> Clon  </span></p>
+
−
<br><br>
+
−
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/0/02/T--AHUT_China--829.png" width="450" height="77" alt=""/></div><br>
+
−
    </div>
+
−
<div class="container">
+
−
              <div class="section_title ">
+
−
                <h2 class="title_color">&nbsp;</h2>
+
−
                <h2 class="title_color">&nbsp;&nbsp;<p style="text-align: center; font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 36px;"><strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400; color: #000000;">4 . IIT-Madras</strong></p></h2><hr>
+
−
<nbsp><nbsp>
+
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px; ">&nbsp;&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">As</span> the project continues to advance, HP's exchanges and cooperations between teams are more frequent and in-depth. After seeing the cooperation application issued by the iGEM team of the Indian Institute of Technology (IIT-Madras) at the iGEM official website, we contacted IIT-Madras to discuss cooperation actively.</p>
+
−
                  <p style="text-align: "><span style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px;">&nbsp;&nbsp;&nbsp;&nbsp;We are pleased to collaborate with the iGEM team of the Indian Institute of Technology, Madras (IIT-Madras) to make a video on the science of synthetic biology in the mother tongue. The Indian Institute of Technology is also working with other teams in English, Tamil, Telugu, Kannada, Malayalam, Marathi, Bengali, Gujarati and Hindi. </span></p>
+
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px; ">&nbsp;&nbsp;&nbsp;&nbsp;IIT-Madras sends an English script to our team. We need to match the provided slideshow video with the provided text and add the translated text to the subtitles, and finally make it into Chinese video.</p>
+
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px; text-align: ;">&nbsp;&nbsp;&nbsp;&nbsp;We thought it was a simple matter, but there were some  problems at the beginning.The problem is summarized as follows:</p>
+
−
     
+
−
<p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-ize: 16px; font-size: 16px;text-align: ;">&nbsp;&nbsp;&nbsp;&nbsp;1. The English script does not exactly match the sound in  the video, and some paragraphs are missing.</p>
+
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-ize: 16px; font-size: 16px;text-align: ;">&nbsp;&nbsp;&nbsp;&nbsp;2. English and Chinese belong to different language families. They are slightly different in grammar, and the word order is reversed in translation. <br>
+
−
    </p>
+
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px;text-align: ;">&nbsp;&nbsp;&nbsp;&nbsp;3. Some video pictures and sounds are out of sync and need to be adjusted. </p>
+
−
                    <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px;text-align: ;">&nbsp;&nbsp;&nbsp;&nbsp;4. Our team members are not familiar enough with the Indian accent in English, and some words cannot be completely accurate. </span><br>
+
−
                      <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px;">&nbsp;&nbsp;&nbsp;&nbsp;After consultation and repeated revisions by the iGEM team members of the Anhui University of Technology, four days later, the results were satisfactory and the video was sent to the iGEM team of the Indian Institute of Technology. We have established a good relationship with the iGEM team (IIT-Madras) of the Indian Institute of Technology, Madras.
+
−
                  </p>
+
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px; ">&nbsp;&nbsp;&nbsp;&nbsp;With the successful completion of the HP cooperation, the Anhui University of Technology iGEM team (AHUT-CHINA) looks forward to more cooperation opportunities with the Indian Institute of Technology iGEM team (IIT-Madras).</p>
+
−
                 
+
−
</p>
+
−
                  </p>
+
−
<p>&nbsp;</p>
+
  
−
        <!--================ Recent Area  =================-->
 
−
</section>   
 
−
     
 
−
<p>&nbsp;</p>  <p>&nbsp;</p>   
 
−
        <!--================ start footer Area  =================-->
 
−
        <DIV style="background: rgba(1,1,1,1.00)"><div align="center"><video height="200px" width="800px" controls="controls"><source src="https://static.igem.org/mediawiki/2018/9/91/T--AHUT_China--_foot.mp4" type="video/mp4" ></div>
 
−
                  <br></div>
 
−
     
 
  
 
         <!-- Modals error -->
 
         <!-- Modals error -->
Line 198: Line 140:
  
 
</html>
 
</html>
 +
{{:Team:AHUT_China/footer}}
 +
{{:Team:AHUT_China/footercss}}

Latest revision as of 14:40, 17 October 2018

Royal Hotel

Royal Hotel

>






      The details of the experimental record are as follows: