Difference between revisions of "Team:AHUT China/Notebook"

 
(47 intermediate revisions by 4 users not shown)
Line 1: Line 1:
 
{{:Team:AHUT_China/Template/CSS_RESET}}
 
{{:Team:AHUT_China/Template/CSS_RESET}}
 +
 
<html lang="en">
 
<html lang="en">
 
     <head>
 
     <head>
Line 21: Line 22:
 
      
 
      
 
         <!--================Header Area =================-->
 
         <!--================Header Area =================-->
−
            <header class="header_area">
+
            <header class="header_area">
 
             <div class="container">
 
             <div class="container">
 
                 <nav class="navbar navbar-expand-lg navbar-light">
 
                 <nav class="navbar navbar-expand-lg navbar-light">
 
                     <!-- Brand and toggle get grouped for better mobile display -->
 
                     <!-- Brand and toggle get grouped for better mobile display -->
−
                     <a class="navbar-brand logo_h" href="https://2018.igem.org/Team:AHUT_China/home"><img src="https://static.igem.org/mediawiki/2018/e/ec/T--AHUT_China--_logo.jpg" height="100" width="80" alt=""></a>
+
                     <a class="navbar-brand logo_h" href="https://2018.igem.org/Team:AHUT_China/home"><img src="https://static.igem.org/mediawiki/2018/b/b0/T--AHUT_China--_newlogo.jpg" height="110" width="80" alt=""></a>
 
                     <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#navbarSupportedContent" aria-controls="navbarSupportedContent" aria-expanded="false" aria-label="Toggle navigation">
 
                     <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#navbarSupportedContent" aria-controls="navbarSupportedContent" aria-expanded="false" aria-label="Toggle navigation">
 
                         <span class="icon-bar"></span>
 
                         <span class="icon-bar"></span>
Line 34: Line 35:
 
                     <div class="collapse navbar-collapse offset" id="navbarSupportedContent">
 
                     <div class="collapse navbar-collapse offset" id="navbarSupportedContent">
 
                         <ul class="nav navbar-nav menu_nav ml-auto">
 
                         <ul class="nav navbar-nav menu_nav ml-auto">
−
                             <li class="nav-item active"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/home">Home</a></li>  
+
                             <li class="nav-item "><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/home">Home</a></li>  
 
                             <li class="nav-item submenu dropdown">
 
                             <li class="nav-item submenu dropdown">
 
                                 <a href="#" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">TEAM</a>
 
                                 <a href="#" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">TEAM</a>
Line 41: Line 42:
 
">Team Members</a></li>
 
">Team Members</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Attributions">Attribution</a>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Attributions">Attribution</a>
−
<li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Sponsoring">Sponsoring</a></li>
+
 
                                 </ul>
 
                                 </ul>
 
                             </li>  
 
                             </li>  
 
                             <li class="nav-item submenu dropdown">
 
                             <li class="nav-item submenu dropdown">
−
                                 <a href="#" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">PROJECT</a>
+
                                 <a href="#" style="color:#52C5FD" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">PROJECT</a>
 
                                 <ul class="dropdown-menu">
 
                                 <ul class="dropdown-menu">
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Overview">Overview</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Overview">Overview</a></li>
 +
                                    <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Background">Background</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/protocol">Protocol</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/protocol">Protocol</a></li>
 
                                     <li class="nav-item"><a class="nav-link"
 
                                     <li class="nav-item"><a class="nav-link"
−
href="https://2018.igem.org/Team:AHUT_China/design">Design</a></li>
+
href="https://2018.igem.org/Team:AHUT_China/design">Design</a></li>                                                    
−
                                    <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Background">Background</a></li>
+
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Demonstrate">Demonstrate</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Demonstrate">Demonstrate</a></li>
 
    <li class="nav-item"><a class="nav-link" href="
 
    <li class="nav-item"><a class="nav-link" href="
 
https://2018.igem.org/Team:AHUT_China/InterLab">Interlab</a></li>
 
https://2018.igem.org/Team:AHUT_China/InterLab">Interlab</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Notebook">Notebook</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Notebook">Notebook</a></li>
 +
                                    <li class="nav-item"><a class="nav-link"
 +
href="https://2018.igem.org/Team:AHUT_China/Result">Result</a></li>
 
                                   </li>
 
                                   </li>
 
                                 </ul>
 
                                 </ul>
Line 74: Line 77:
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/HP_FOR_SILVER">HP for Silver</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/HP_FOR_SILVER">HP for Silver</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/INTEGRATED_FOR_GOLD">Integrated HP for gold</a></li>
 
                                     <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/INTEGRATED_FOR_GOLD">Integrated HP for gold</a></li>
 +
                                    <li class="nav-item"><a class="nav-link" href="
 +
https://2018.igem.org/Team:AHUT_China/Public_Engagement">Public Engagement</a></li>
 
                                 </ul>
 
                                 </ul>
 
                             </li>  
 
                             </li>  
Line 82: Line 87:
 
                 </nav>
 
                 </nav>
 
             </div>
 
             </div>
−
         
+
     
 
         </header>
 
         </header>
−
       
+
  <?<div align="center"></div>>       
−
       
+
−
       
+
 
         <!--================Header Area =================-->
 
         <!--================Header Area =================-->
 
        
 
        
Line 93: Line 96:
 
         <!--================Breadcrumb Area =================-->
 
         <!--================Breadcrumb Area =================-->
 
         <!--================ Latest Blog Area  =================-->
 
         <!--================ Latest Blog Area  =================-->
−
         <section class="latest_blog_area section_gap">
+
 
−
            <div class="container">
+
         <!--================ start footer Area  =================-->
−
              <div class="section_title ">
+
 
−
                <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;"> 8.29</strong></h2>
+
      <div align="center">    
−
                <hr>
+
  <br><br><br><br><br><br>
−
                <nbsp><nbsp>
+
<p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 28px; color: rgba(248,6,6,1);center" >
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 14px;"> <span style="font-size: 16px">&nbsp;&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">&nbsp;Pick</span> Clon  </span></p>
+
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The details of the experimental record are as follows:<br></p>
−
<br><br>
+
<iframe src="https://static.igem.org/mediawiki/2018/9/9b/T--AHUT_China--Notebook.pdf" width="1000" height="800"></iframe>
−
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/0/02/T--AHUT_China--829.png" width="450" height="77" alt=""/></div><br>
+
</div>
−
    </div>
+
−
<div class="container">
+
−
              <div class="section_title ">
+
−
                  <p style="text-align: center; font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 36px;"><strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400; color: #000000;">8.30</strong></p>
+
−
                </h2>
+
−
                <hr>
+
−
                 
+
−
                  <nbsp><nbsp>
+
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px; ">&nbsp;&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">Pilot </span> Expression of Protein<br>&nbsp;&nbsp;&nbsp;“IPTG+ 4h”“IPTG- 4h”</p> 
+
−
<p>&nbsp;</p> <p>&nbsp;</p>             
+
−
 
+
−
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/4/44/T--AHUT_China--830.png" width="420" height="77" alt=""/></div><br><p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px;">&nbsp;&nbsp;&nbsp;After the rocker culture is 4 H, add IPTG(final concentration is 0.5 mm) and then the rocker culture is 4 hours. Each of the two tubes absorbs 200 μL of bacterial fluid, and in the frozen centrifuge, it is 4 °C and 12000 rpm centrifuge. 10 minutes, go to the clear, It is resuspended with 100 μL 1x SDS Loading buffer, then heated in a water bath pot at 100 °C for 10 minutes and stored in a -20 °C refrigerator.</p>
+
−
          </div>
+
−
                        <div class="container">
+
−
              <div class="section_title ">
+
−
                <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;"> 8.31</strong></h2>
+
−
                <hr>
+
−
                <nbsp><nbsp>
+
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 16px;">&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">&nbsp;Protein</span> extraction <br>&nbsp;&nbsp;&nbsp;&nbsp;CA2L203K</p> 
+
−
<p>&nbsp;</p> <p>&nbsp;</p> 
+
−
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/2/2d/T--AHUT_China--831.11.png" width="761" height="76" alt=""/></div>
+
−
<div align="center"><img src="https://static.igem.org/mediawiki/2018/e/eb/T--AHUT_China--831.12.png" width="657" height="476" alt=""/></div>
+
−
<p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 16px;">&nbsp;&nbsp;&nbsp;&nbsp;CA2WT<br>&nbsp;&nbsp;&nbsp;&nbsp;CA2L203K</p>
+
−
<div align="center"><img src="https://static.igem.org/mediawiki/2018/e/ee/T--AHUT_China--831.2.png" width="998" height="75" alt=""/></div>
+
−
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/3/3f/T--AHUT_China--831.22.png" width="703" height="497" alt=""/></div>
+
−
<br>
+
−
        <div class="container">
+
−
              <div class="section_title ">
+
−
                <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;"> 9.01</strong></h2>
+
−
                <hr>
+
−
                <nbsp><nbsp>
+
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 14px;"> <span style="font-size: 16px">&nbsp;&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">Purification</span>&nbsp;of Protein <br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Prior to purification</p>
+
−
<br>
+
−
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/c/cc/T--AHUT_China--91.1.png" width="974" height="74" alt=""/></div>
+
−
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/7/7b/T--AHUT_China--91.2.png" width="657" height="476" alt=""/></div>
+
−
  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 14px;"> <span style="font-size: 16px">&nbsp;&nbsp;&nbsp;&nbsp;Purified</p><br>
+
−
<div align="center"><img src="https://static.igem.org/mediawiki/2018/8/8a/T--AHUT_China--91.3.png" width="564" height="75" alt=""/></div>
+
−
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/6/65/T--AHUT_China--91.4.png" width="373" height="431" alt=""/></div><br>
+
−
<div class="container">
+
−
              <div class="container">
+
−
              <div class="section_title ">
+
−
                <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;">9.03</strong></h2>
+
−
                <hr>
+
−
                <nbsp><nbsp>
+
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px;">&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">&nbsp;Western</span> blot identification of proteins<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;CA2L203K&nbsp;&nbsp;&nbsp;&nbsp;CA2WT</p>  <br>
+
−
<div align="center"><img src="https://static.igem.org/mediawiki/2018/6/60/T--AHUT_China--93.1.png" width="971" height="167" alt=""/></div>               
+
−
<div class="container">
+
−
              <div class="section_title ">
+
−
                <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;">9.04</strong></h2>
+
−
                <hr>
+
−
                <nbsp><nbsp>
+
−
                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 16px;">&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">&nbsp;Quantification</span> of protein concentrations</p>  
+
−
<br>
+
−
<div align="center"><img src="https://static.igem.org/mediawiki/2018/6/6e/T--AHUT_China--93.2.png" width="908" height="189" alt=""/></div>
+
−
<div align="center"><img src="https://static.igem.org/mediawiki/2018/8/8b/T--AHUT_China--93.3.png" width="754" height="343" alt=""/></div>
+
  
−
        <!--================ Recent Area  =================-->
 
−
</section>   
 
−
     
 
−
<p>&nbsp;</p>  <p>&nbsp;</p>   
 
−
        <!--================ start footer Area  =================-->
 
−
        <DIV style="background: rgba(1,1,1,1.00)"><div align="center"><video height="200px" width="800px" controls="controls"><source src="https://static.igem.org/mediawiki/2018/9/91/T--AHUT_China--_foot.mp4" type="video/mp4" ></div>
 
−
                  <br></div>
 
−
     
 
  
 
         <!-- Modals error -->
 
         <!-- Modals error -->
Line 200: Line 140:
  
 
</html>
 
</html>
 +
{{:Team:AHUT_China/footer}}
 +
{{:Team:AHUT_China/footercss}}

Latest revision as of 14:40, 17 October 2018

Royal Hotel

Royal Hotel

>






      The details of the experimental record are as follows: