Difference between revisions of "Team:AHUT China/Notebook"

Latest revision as of 14:40, 17 October 2018

Royal Hotel

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 {{:Team:AHUT_China/Template/CSS_RESET}}
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+         <link rel="stylesheet" href="https://2018.igem.org/Team:AHUT_China/nice-select_css?action=raw&ctype=text/css" type="text/css" />
+        <link rel="stylesheet" href="https://2018.igem.org/Team:AHUT_China/owl.carousel.min_css?action=raw&ctype=text/css" type="text/css" />
+        <!-- main css -->
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+        <link rel="stylesheet" href="https://2018.igem.org/Team:AHUT_China/responsive_css?action=raw&ctype=text/css" type="text/css" />
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+        <!--================Header Area =================-->
+             <header class="header_area">
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                              <li class="nav-item "><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/home">Home</a></li>
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-                                 <a href="https://2018.igem.org/Team:AHUT_China/Team"  class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> TEAM</a>
+                                 <a href="#" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">TEAM</a>
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                                      <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/HP_FOR_SILVER">HP for Silver</a></li>
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+                                    <li class="nav-item"><a class="nav-link" href="
+https://2018.igem.org/Team:AHUT_China/Public_Engagement">Public Engagement</a></li>
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                  </nav>
              </div>
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+   <?<div align="center"></div>>
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-    </head>
-        <!--================Header Area =================-->
          <!--================Header Area =================-->
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          <!--================Breadcrumb Area =================-->
          <!--================ Latest Blog Area  =================-->
-         <section class="latest_blog_area section_gap">
-            <div class="container">
+         <!--================ start footer Area  =================-->
-              	<div class="section_title ">
-                 <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;">Notebook</strong></h2>
+       <div align="center">
-                <hr>
+  <br><br><br><br><br><br>
-                <nbsp><nbsp><br><br><br>
+  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 28px; color: rgba(248,6,6,1);center" >
-                 <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;"> 8.29</strong></h2>
+&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The details of the experimental record are as follows:<br></p>
-                <hr>
+<iframe src="https://static.igem.org/mediawiki/2018/9/9b/T--AHUT_China--Notebook.pdf" width="1000" height="800"></iframe>
-                <nbsp><nbsp>
+</div>
-                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 14px;"> <span style="font-size: 16px">&nbsp;&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">&nbsp;Pick</span> Clon  </span></p>
-<br><br>
-  <div align="center"><img src="https://static.igem.org/mediawiki/2018/0/02/T--AHUT_China--829.png" width="450" height="77" alt=""/></div><br>
-    </div>
-			 <div class="container">
-              	<div class="section_title ">
-                  <p style="text-align: center; font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 36px;"><strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400; color: #000000;">8.30</strong></p>
-                </h2>
-                <hr>
-                  <nbsp><nbsp>
-                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px; ">&nbsp;&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">Pilot </span> Expression of Protein<br>&nbsp;&nbsp;&nbsp;“IPTG+ 4h”“IPTG- 4h”</p>
-<p>&nbsp;</p> <p>&nbsp;</p>
-			 	  	<div align="center"><img src="https://static.igem.org/mediawiki/2018/4/44/T--AHUT_China--830.png" width="420" height="77" alt=""/></div><br><p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px;">&nbsp;&nbsp;&nbsp;After the rocker culture is 4 H, add IPTG(final concentration is 0.5 mm) and then the rocker culture is 4 hours. Each of the two tubes absorbs 200 μL of bacterial fluid, and in the frozen centrifuge, it is 4 °C and 12000 rpm centrifuge. 10 minutes, go to the clear, It is resuspended with 100 μL 1x SDS Loading buffer, then heated in a water bath pot at 100 °C for 10 minutes and stored in a -20 °C refrigerator.</p>
-          </div>
-                        <div class="container">
-              	<div class="section_title ">
-                 <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;"> 8.31</strong></h2>
-                <hr>
-                <nbsp><nbsp>
-                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 18px;">&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">&nbsp;Protein</span> extraction <br>&nbsp;&nbsp;&nbsp;&nbsp;CA2L203K</p>
-<p>&nbsp;</p> <p>&nbsp;</p>
-  <div align="center"><img src="https://static.igem.org/mediawiki/2018/2/2d/T--AHUT_China--831.11.png" width="761" height="76" alt=""/></div>
- <div align="center"><img src="https://static.igem.org/mediawiki/2018/e/eb/T--AHUT_China--831.12.png" width="657" height="476" alt=""/></div>
-<p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 16px;">&nbsp;&nbsp;&nbsp;&nbsp;CA2WT<br>&nbsp;&nbsp;&nbsp;&nbsp;CA2L203K</p>
- <div align="center"><img src="https://static.igem.org/mediawiki/2018/e/ee/T--AHUT_China--831.2.png" width="998" height="75" alt=""/></div>
- <div align="center"><img src="https://static.igem.org/mediawiki/2018/3/3f/T--AHUT_China--831.22.png" width="703" height="497" alt=""/></div>
-<br><br><br>
-         <div class="container">
-              	<div class="section_title ">
-                 <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;"> 9.01</strong></h2>
-                <hr>
-                <nbsp><nbsp>
-                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 14px;"> <span style="font-size: 16px">&nbsp;&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">Purification</span>&nbsp;of Protein <br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Prior to purification</p>
-<br>
-  <div align="center"><img src="https://static.igem.org/mediawiki/2018/c/cc/T--AHUT_China--91.1.png" width="974" height="74" alt=""/></div>
-  <div align="center"><img src="https://static.igem.org/mediawiki/2018/7/7b/T--AHUT_China--91.2.png" width="657" height="476" alt=""/></div>
-  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 14px;"> <span style="font-size: 16px">&nbsp;&nbsp;&nbsp;&nbsp;Purified</p><br>
-<div align="center"><img src="https://static.igem.org/mediawiki/2018/8/8a/T--AHUT_China--91.3.png" width="564" height="75" alt=""/></div>
-  <div align="center"><img src="https://static.igem.org/mediawiki/2018/6/65/T--AHUT_China--91.4.png" width="373" height="431" alt=""/></div><br>
-	<br><br>		<div class="container">
-              	 <div class="container">
-              	<div class="section_title ">
-                 <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;">9.03</strong></h2>
-                <hr>
-                <nbsp><nbsp>
-                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 18px;">&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">&nbsp;Western</span> blot identification of proteins<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;CA2L203K&nbsp;&nbsp;&nbsp;&nbsp;CA2WT</p>   <br>
-  <div align="center"><img src="https://static.igem.org/mediawiki/2018/6/60/T--AHUT_China--93.1.png" width="971" height="167" alt=""/></div> <br>
-<br><br>
- <div class="container">
-              	<div class="section_title ">
-                 <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;">9.04</strong></h2>
-                <hr>
-                <nbsp><nbsp>
-                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 18px;">&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">&nbsp;Quantification</span> of protein concentrations</p>
-<br>
-<div align="center"><img src="https://static.igem.org/mediawiki/2018/6/6e/T--AHUT_China--93.2.png" width="908" height="189" alt=""/></div>
-<div align="center"><img src="https://static.igem.org/mediawiki/2018/8/8b/T--AHUT_China--93.3.png" width="754" height="343" alt=""/></div><br>
-<br><br>
-<div class="container">
-              	<div class="section_title ">
-                 <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;">  &nbsp;&nbsp;&nbsp;<strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;">9.05</strong></h2>
-                <hr>
-                <nbsp><nbsp>
-                  <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 18px;">&nbsp;&nbsp;&nbsp;<span style="font-size: 24px">&nbsp;Colorimetric</span> enzyme activity<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Add 250 μl saturated CO2 solution</p>
-<br>
-<div align="center"><img src="https://static.igem.org/mediawiki/2018/c/c4/T--AHUT_China--95.1.png" width="789" height="328" alt=""/></div>
-<div align="center"><img src="https://static.igem.org/mediawiki/2018/8/82/T--AHUT_China--95.2.png" width="669" height="347" alt=""/></div>
- <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 18px;">&nbsp;&nbsp;&nbsp;According to the average calculated in the table above, the unit enzyme activity of the mutated carbonic anhydride enzyme is 0.3710(10 5 WAU/μg), and the unit enzyme activity of wild carbonic anhydride enzyme is 0.1755(10 5 WAU/μg).<br>
-Test esterase activity<br>
-Determination curve without carbonated anhydrase:</p> <br>
-<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/f6/T--AHUT_China--95.3.png" width="903" height="296" alt=""/></div><br>
-<p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 18px;">&nbsp;&nbsp;&nbsp;Determination curve with carbonated anhydrase（CA2WT）</p> <br>
-<div align="center"><img src="https://static.igem.org/mediawiki/2018/e/e8/T--AHUT_China--95.4.png" width="929" height="330" alt=""/></div>
-<p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 18px;">&nbsp;&nbsp;&nbsp;Determination curve with carbonated anhydrase（CA2L203K）</p> <br>
-<div align="center"><img src="https://static.igem.org/mediawiki/2018/0/0c/T--AHUT_China--95.5.png" width="893" height="306" alt=""/></div>
-<p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 18px;">&nbsp;&nbsp;&nbsp;Wild carbonic anhydrase activity calculated as 0.5098 mol/g × min<br>
-&nbsp;&nbsp;&nbsp;The mutated carbonic anhydrase enzyme activity was calculated to be 1.0392 mol/g * min.
-</p> <br>
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