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| | <div class="collapse navbar-collapse offset" id="navbarSupportedContent"> | | <div class="collapse navbar-collapse offset" id="navbarSupportedContent"> |
| | <ul class="nav navbar-nav menu_nav ml-auto"> | | <ul class="nav navbar-nav menu_nav ml-auto"> |
| − | <li class="nav-item active"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/home">Home</a></li> | + | <li class="nav-item "><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/home">Home</a></li> |
| | <li class="nav-item submenu dropdown"> | | <li class="nav-item submenu dropdown"> |
| | <a href="#" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">TEAM</a> | | <a href="#" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">TEAM</a> |
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| | </li> | | </li> |
| | <li class="nav-item submenu dropdown"> | | <li class="nav-item submenu dropdown"> |
| − | <a href="#" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">PROJECT</a> | + | <a href="#" style="color:#52C5FD" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">PROJECT</a> |
| | <ul class="dropdown-menu"> | | <ul class="dropdown-menu"> |
| | <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Overview">Overview</a></li> | | <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Overview">Overview</a></li> |
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| | <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/INTEGRATED_FOR_GOLD">Integrated HP for gold</a></li> | | <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/INTEGRATED_FOR_GOLD">Integrated HP for gold</a></li> |
| | <li class="nav-item"><a class="nav-link" href=" | | <li class="nav-item"><a class="nav-link" href=" |
| − | https://2018.igem.org/Team:AHUT_China/Public_Engagement">Public_Engagement</a></li> | + | https://2018.igem.org/Team:AHUT_China/Public_Engagement">Public Engagement</a></li> |
| | </ul> | | </ul> |
| | </li> | | </li> |
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| | </nav> | | </nav> |
| | </div> | | </div> |
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| | </header> | | </header> |
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| + | <?<div align="center"></div>> |
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| | <!--================Header Area =================--> | | <!--================Header Area =================--> |
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| | <!--================Breadcrumb Area =================--> | | <!--================Breadcrumb Area =================--> |
| | <!--================ Latest Blog Area =================--> | | <!--================ Latest Blog Area =================--> |
| − | <section class="latest_blog_area section_gap"> | + | |
| − | <div class="container">
| + | <!--================ start footer Area =================--> |
| − | <div class="section_title ">
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;"> <strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;">Notebook</strong></h2>
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| − | <hr>
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| − | <nbsp><nbsp><br><br><br>
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 14px;"> <span style="font-size: 18px">Wild-type CA2</span> </p></h2><hr>
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| − | <br><br>
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| | | | |
| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;> <strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;"> 8.21</strong></h2>
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| − | <hr>
| + | <br><br><br><br><br><br> |
| − | <nbsp><nbsp>
| + | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 28px; color: rgba(248,6,6,1);center" > |
| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;"> <strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;"> 8.29</strong></h2>
| + | The details of the experimental record are as follows:<br></p> |
| − | <hr>
| + | <iframe src="https://static.igem.org/mediawiki/2018/9/9b/T--AHUT_China--Notebook.pdf" width="1000" height="800"></iframe> |
| − | <nbsp><nbsp>
| + | </div> |
| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 14px;"> <span style="font-size: 18px"> <span style="font-size: 24px">Pick</span> Clon</span></p>
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| − | <br><br>
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| − | <div align="center"> <img src="https://static.igem.org/mediawiki/2018/0/02/T--AHUT_China--829.png" width="650" alt=""/></div><br> | + | |
| − | </div>
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| − | <br><br>
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| − | <div class="container">
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| − | <div class="section_title ">
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| − | <p style="text-align: center; font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 36px;"><strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400; color: #000000;">8.30</strong></p>
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| − | </h2>
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| − | <hr>
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| − | <nbsp><nbsp>
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 18px; "> <span style="font-size: 24px">Pilot</span> Expression of Protein<br> “IPTG+ 4h”“IPTG- 4h”</p>
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| − | <p> </p> <p> </p>
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| − |
| + | |
| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/4/44/T--AHUT_China--830.png" width="650" alt=""/></div><br><br><br><p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 18px;"> After the rocker culture is 4 H, add IPTG(final concentration is 0.5 mm) and then the rocker culture is 4 hours. Each of the two tubes absorbs 200 μL of bacterial fluid, and in the frozen centrifuge, it is 4 °C and 12000 rpm centrifuge. 10 minutes, go to the clear, It is resuspended with 100 μL 1x SDS Loading buffer, then heated in a water bath pot at 100 °C for 10 minutes and stored in a -20 °C refrigerator.</p>
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| − | </div>
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| − | <br><br><br><br><br> | + | |
| − | <div class="container">
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| − | <div class="section_title ">
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;"> <strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;">8.31</strong></h2>
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| − | <hr>
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| − | <nbsp><nbsp>
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 18px;"> <span style="font-size: 24px"> Protein</span> extraction <br> CA2L203K</p> | + | |
| − | <p> </p> <p> </p>
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| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/2/2d/T--AHUT_China--831.11.png" width="800" alt=""/></div>
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| − | <br><br>
| + | |
| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/e/eb/T--AHUT_China--831.12.png" width="800" alt=""/></div>
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 18px;"> CA2WT<br> CA2L203K</p>
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| − | <br><br>
| + | |
| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/e/ee/T--AHUT_China--831.2.png" width="998" alt=""/></div>
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| − | <br><br>
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| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/3/3f/T--AHUT_China--831.22.png" width="800" alt=""/></div>
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| − | <br><br><br><br><br>
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| − | <div class="container">
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| − | <div class="section_title ">
| + | |
| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;"> <strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;"> 9.01</strong></h2>
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| − | <hr>
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| − | <nbsp><nbsp>
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 14px;"> <span style="font-size: 18px"> <span style="font-size: 24px">Purification</span> of Protein <br> Prior to purification</p>
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| − | <br>
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| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/c/cc/T--AHUT_China--91.1.png" width="974" alt=""/></div>
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| − | <br><br>
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| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/7/7b/T--AHUT_China--91.2.png" width="800" alt=""/></div>
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| − | <br><br><br>
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 14px;"> <span style="font-size: 18px">Purified</p><br><br><br>
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| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/8/8a/T--AHUT_China--91.3.png" width="564" height="75" alt=""/></div>
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| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/6/65/T--AHUT_China--91.4.png" width="450" alt=""/></div><br>
| + | |
| − | <br><br><br><br>
| + | |
| − | <div class="container">
| + | |
| − | <div class="container">
| + | |
| − | <div class="section_title ">
| + | |
| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;"> <strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;">9.03</strong></h2>
| + | |
| − | <hr>
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| − | <nbsp><nbsp>
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 18px;"> <span style="font-size: 24px"> Western</span> blot identification of proteins<br> CA2L203K CA2WT</p>
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| − | <br><br><br>
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| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/6/60/T--AHUT_China--93.1.png" width="9" alt=""/></div> <br>
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| − | <br><br><br><br><br>
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| − | <div class="container">
| + | |
| − | <div class="section_title ">
| + | |
| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;"> <strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;">9.04</strong></h2>
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| − | <hr>
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| − | <nbsp><nbsp>
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 18px;"> <span style="font-size: 24px"> Quantification</span> of protein concentrations</p>
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| − | <br> | + | |
| − | <br><br>
| + | |
| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/6/6e/T--AHUT_China--93.2.png" width="908" alt=""/></div>
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| − | <br><br>
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| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/8/8b/T--AHUT_China--93.3.png" width="850" alt=""/></div><br>
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| − | <br><br><br><br><br>
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| − | <div class="container">
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| − | <div class="section_title ">
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;"> <strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;">9.05</strong></h2>
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| − | <hr>
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| − | <nbsp><nbsp>
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 18px;"> <span style="font-size: 24px"> Colorimetric</span> enzyme activity<br> Add 250 μl saturated CO2 solution</p>
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| − | <br><br>
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| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/c/c4/T--AHUT_China--95.1.png" width="800" alt=""/></div>
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| − | <br><br><br>
| + | |
| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/8/82/T--AHUT_China--95.2.png" width="800" alt=""/></div>
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| − | <br><br><br> | + | |
| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 18px;"> According to the average calculated in the table above, the unit enzyme activity of the mutated carbonic anhydride enzyme is 0.3710(10 5 WAU/μg), and the unit enzyme activity of wild carbonic anhydride enzyme is 0.1755(10 5 WAU/μg).<br>
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| − | Test esterase activity<br>
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| − | Determination curve without carbonated anhydrase:</p> <br>
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| − | <br><br>
| + | |
| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/f/f6/T--AHUT_China--95.3.png" width="900" alt=""/></div><br><br>
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 18px;"> Determination curve with carbonated anhydrase（CA2WT）</p> <br><br>
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| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/e/e8/T--AHUT_China--95.4.png" width="900" alt=""/></div>
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 18px;"> Determination curve with carbonated anhydrase（CA2L203K）</p> <br><br>
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| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/0/0c/T--AHUT_China--95.5.png" width="900" alt=""/></div>
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| − | <br><br><br>
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| − | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 18px;"> Wild carbonic anhydrase activity calculated as 0.5098 mol/g × min<br>
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| − | The mutated carbonic anhydrase enzyme activity was calculated to be 1.0392 mol/g * min.
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| − | </p> <br>
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