JackBechtel (Talk | contribs) |
|||
(24 intermediate revisions by 2 users not shown) | |||
Line 1: | Line 1: | ||
{{WLC-Milwaukee/header}} | {{WLC-Milwaukee/header}} | ||
+ | {{WLC-Milwaukee/CSS}} | ||
+ | <html> | ||
+ | <head lang="en"> | ||
+ | <meta charset="utf-8"> | ||
+ | <meta http-equiv="x-ua-compatible" content="IE=edge; chrome=1"> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | ||
+ | <!--IMPORT CSS--> | ||
+ | <link href="fonts.css" rel="stylesheet"> | ||
+ | <link href="main.css" rel="stylesheet"> | ||
+ | <!--IMPORT JQUERY--> | ||
+ | <script src="jquery.js.txt"></script> | ||
+ | |||
+ | <script> | ||
+ | //Include Header and Footer Files using jQuery | ||
+ | $(function(){ | ||
+ | $("#header").load("header.html"); | ||
+ | $("#footer").load("footer.html"); | ||
+ | }); | ||
+ | </script> | ||
+ | |||
+ | <style> | ||
+ | #img{ | ||
+ | width: 100%; | ||
+ | height: auto; | ||
+ | } | ||
+ | .centered{ | ||
+ | text-align: center; | ||
+ | } | ||
+ | |||
+ | .tile-img{ | ||
+ | /*set background image in html*/ | ||
+ | background-size: 100% 100%; | ||
+ | transition: all 0.3s ease; | ||
+ | display: inline-block; | ||
+ | padding-top: 41%; | ||
+ | padding-bottom: 41%; | ||
+ | margin: auto; | ||
+ | margin-top: 0.75em; | ||
+ | text-align: center; | ||
+ | font-size: 1.5em; | ||
+ | color: white; | ||
+ | text-decoration: none !important; | ||
+ | font-family: 'Arimo', sans-serif; | ||
+ | font-weight: bold; | ||
+ | width: calc(100%); | ||
+ | background-color: rgba(29, 19, 57, 0.8); | ||
+ | background-blend-mode: overlay; | ||
+ | } | ||
+ | |||
+ | .tile-img:visited{color: white !important;} | ||
+ | |||
+ | .tile-img:hover { | ||
+ | transition: all 0.3s ease; | ||
+ | background-color: rgba(29, 19, 57, 0.3); | ||
+ | box-shadow: 0 14px 28px rgba(0,0,0,0.5), 0 10px 10px rgba(0,0,0,0.5); | ||
+ | } | ||
+ | |||
+ | hr.left{ | ||
+ | float: left; | ||
+ | } | ||
+ | |||
+ | </style> | ||
+ | |||
+ | <!--PAGE TITLE--> | ||
+ | <title>WLC iGEM 2018 | Project</title> | ||
+ | </head> | ||
+ | |||
+ | |||
+ | <body> | ||
+ | |||
+ | <!--BEGIN HEADER IMPORT--> | ||
+ | <div id="header"></div> | ||
+ | <!--END HEADER IMPORT--> | ||
+ | |||
+ | <div class="content"> | ||
+ | <!--CONTENT CONTAINER--> | ||
+ | <div class="container"> | ||
+ | <div class="row centered header-image-container" style="background-image: url(https://static.igem.org/mediawiki/2018/0/09/T--WLC-Milwaukee--ProjectOverviewHeader.jpeg)"> | ||
+ | <div class="header-image-text"> | ||
+ | <h1>Overview</h1> | ||
+ | </div> | ||
+ | <img id="img" src="https://static.igem.org/mediawiki/2018/0/09/T--WLC-Milwaukee--ProjectOverviewHeader.jpeg"> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="row"> | ||
+ | <p>This year, WLC Milwaukee will be continuing to expand and improve our previous project developing an easy to use test kit for E. coli water contamination that is rapid, efficient, and accurate. By using expressed and purified Lambda Phage J-Protein, which is known to bind E. coli LamB, conjugated to an enzyme, we can detect E. coli in a water sample by addition of a chromagenic substrate cleaved by the conjugated enzyme of choice. This allows the kit to be customized to the needs of the consumer, whether they are a corporation or an individual, simply by changing the conjugated enzyme or substrate used. We believe this simple system of bacteriophage binding protein and enzyme conjugation can be expanded and used to detect other pollution indicating species or potential pathogens in water supplies. | ||
+ | </p> | ||
+ | </div> | ||
+ | |||
+ | <div id="tile_container"class="row"> | ||
+ | <div class="col-sm"> | ||
+ | <a class="tile-img" | ||
+ | style="background-image: url(https://static.igem.org/mediawiki/2018/5/54/T--WLC-Milwaukee--BackgroundSquare.jpeg);" | ||
+ | href="/Team:WLC-Milwaukee/Background">BACKGROUND | ||
+ | </a> | ||
+ | </div> | ||
+ | <div id="system_design" class="col-sm"> | ||
+ | <a class="tile-img" | ||
+ | style="background-image: url(https://static.igem.org/mediawiki/2018/4/48/T--WLC-Milwaukee--SystemDesignSquare.jpeg);" | ||
+ | href="/Team:WLC-Milwaukee/Applied_Design">SYSTEM DESIGN | ||
+ | </a> | ||
+ | </div> | ||
+ | <div class="col-sm"> | ||
+ | <a class="tile-img" | ||
+ | style="background-image: url(https://static.igem.org/mediawiki/2018/4/4a/T--WLC-Milwaukee--WetLabFooterSquare.jpeg);" | ||
+ | href="/Team:WLC-Milwaukee/Experiments">WETLAB | ||
+ | </a> | ||
+ | </div> | ||
+ | <div class="col-sm"> | ||
+ | <a class="tile-img" | ||
+ | style="background-image: url(https://static.igem.org/mediawiki/2018/d/dd/T--WLC-Milwaukee--ResultsFooterSquare.jpeg);" | ||
+ | href="/Team:WLC-Milwaukee/Demonstrate">RESULTS | ||
+ | </a> | ||
+ | </div> | ||
+ | <div class="col-sm"> | ||
+ | <a class="tile-img" | ||
+ | style="background-image: url(https://static.igem.org/mediawiki/2018/4/41/T--WLC-Milwaukee--PartsSquare.jpeg);" | ||
+ | href="/Team:WLC-Milwaukee/Improve">PARTS | ||
+ | </a> | ||
+ | </div> | ||
+ | <div class="col-sm"> | ||
+ | <a class="tile-img" | ||
+ | style="background-image: url(https://static.igem.org/mediawiki/2018/4/48/T--WLC-Milwaukee--TimelineSquare.jpeg);" | ||
+ | href="/Team:WLC-Milwaukee/Notebook">TIMELINE | ||
+ | </a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!--END CONTENT CONTAINER--> | ||
+ | </div> | ||
+ | |||
+ | <!--BEGIN FOOTER IMPORT--> | ||
+ | <div id="footer"></div> | ||
+ | <!--END FOOTER IMPORT--> | ||
+ | |||
+ | </body> | ||
+ | </html> | ||
{{WLC-Milwaukee/footer}} | {{WLC-Milwaukee/footer}} |
Latest revision as of 15:14, 17 October 2018
Overview
This year, WLC Milwaukee will be continuing to expand and improve our previous project developing an easy to use test kit for E. coli water contamination that is rapid, efficient, and accurate. By using expressed and purified Lambda Phage J-Protein, which is known to bind E. coli LamB, conjugated to an enzyme, we can detect E. coli in a water sample by addition of a chromagenic substrate cleaved by the conjugated enzyme of choice. This allows the kit to be customized to the needs of the consumer, whether they are a corporation or an individual, simply by changing the conjugated enzyme or substrate used. We believe this simple system of bacteriophage binding protein and enzyme conjugation can be expanded and used to detect other pollution indicating species or potential pathogens in water supplies.