Difference between revisions of "Team:JNFLS/Parts"

Fig.1 Map of pCold Ⅱvector shows TEE and His tag are inserted to the upstream of multiple cloning sites.

Fig.2 Diagram of pCold Ⅱvector shows the cloning site of HCV C gene insertion.

We constructed 10 HCV core protein parts with different length and different tag, even different sequences due to the codon optimization. All the part information are summarized in the table below.

In these parts, the BBa_K2608004 was the shortest one which contains 360bp, encoding 120aa without tagging other sequence. It was also optimized and expressed very well, which showed as following.

Fig.3 The centrifuge pellets of bacteria shows the induced expression of fusion HCV C protein in E.coli

Fig.4 SDS-PAGE result showing the expression of HCV C-O173 and HCV C-O120, which were marked by GFP

You can view our parts here:

<groupparts>iGEM18 JNFLS</groupparts>