Difference between revisions of "Team:Vilnius-Lithuania/Human Practices"

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         <h1>Description</h1>
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         <h1>Passing on the Knowledge to the Younger Generation</h1>
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        <p></p>
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<p>Excited by the possibilities synthetic biology has to offer, our team aimed to involve younger generation in this novel field of science by educating them in schools, during pupil-olympiads, and meetings of other societies.</p>
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        <p></p>
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<p>Our team members continued the tradition of previous Vilnius-Lithuania iGEM teams and held lectures for students in different schools across the country. During these visits scholars had an opportunity to learn the main principles of synthetic biology and laboratory work bearing in mind SynDrop project as an example. Also, high-school students have visited our laboratory in Vilnius University Life Sciences Center and implemented their knowledge into practice, e.g. learning how to balance a centrifuge or using Burker camera in order to calculate cells.</p>
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        <h2>What is SynORI?</h2>
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<p>Additionally, we delegated our member Valentas to represent our team in organising the 51st LitBO (Lithuanian Biology Olympiad) and its bootcamp where students were preparing for the International Biology Olympiad. He was responsible for giving lectures about fundamental biological principles and synthetic biology, as well as planning experiments for students.</p>
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        <p>SynORI stands for synthetic origin of replication. It is a framework designed to make working with single
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<p>Our instructor Auksė held a lecture for Turing School students (13-18 yrs. of age) who planned to become future inventors and leaders of the IT sector. She delineated the possibilities of synthetic biology, revealed future applications of CRISPR/Cas system, and most importantly described ways of how IT skills could be applied in bioinformatics and above mentioned fields.</p>>
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            and multi-plasmid systems precise, easy and on top of that - more functional.</p>
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        <p>The SynORI framework enables scientists to build a multi-plasmid system in a standardized manner by:</p>
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        <ol>
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            <li>Selecting the number of plasmid groups</li>
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            <li>Choosing the copy number of each group</li>
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            <li>Picking the type of copy number control (specific to one group or regulating all of them at once).</li>
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        </ol>
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<p>SILVER photo</p>
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        </p>
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<h1>DNA Day's Celebration</h1>
  
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        <p></p>
 
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        <p>The framework also includes a possibility of adding a selection system that reduces the usage of antibiotics
 
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            (only 1 antibiotic for up to 5 different plasmids!) and an active partitioning system to make sure that low
 
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            copy number plasmid groups are not lost during the division.
 
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        </p>
 
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        <p></p>
 
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            <img src="https://static.igem.org/mediawiki/parts/8/84/Collect.png" alt="img">
 
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        <h2>Applications</h2>
 
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        <p>
 
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            <h5>Everyday lab work</h5>
 
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            <p>
 
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                A multi-plasmid system that is easy to assemble and control. With our framework the need to limit your
 
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                research to a particular plasmid copy number just because there are not enough right replicons to
 
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                choose from, is eliminated. With SynORI you can easily create a vector with a desired copy number that
 
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                suits your needs.</li>
 
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            </p>
 
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            <h5>Biological computing</h5>
 
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            <p>
 
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                The ability to choose a wide range of copy number options and their control types will make the
 
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                synthetic biology engineering much more flexible and predictable. Introduction of plasmid copy number
 
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                regulation is equivalent to adding a global parameter to a computer system. It enables the coordination
 
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                of multiple gene group expression.
 
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            </p>
 
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            <h5>Smart assembly of large protein complexes</h5>
 
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            <p>
 
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                The co-expression of multi-subunit complexes using different replicons brings incoherency to an already
 
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                chaotic cell system. This can be avoided by using SynORI, as in this framework every plasmid group uses
 
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                the same type of control, and in addition can act in a group-specific manner.</p>
 
  
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            <h5>Metabolic engineering</h5>
 
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            <p>
 
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                A big challenge for heterologous expression of multiple gene pathways is to accurately adjust the
 
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                levels of each enzyme to achieve optimal production efficiency. Precise promoter tuning in
 
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                transcriptional control and synthetic ribosome binding sites in translational control are already
 
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                widely used to maintain expression levels. In addition to current approaches, our framework allows a
 
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                simultaneous multiple gene control. Furthermore, an inducible regulation that we offer, can make the
 
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                search for perfect conditions a lot easier.
 
  
  
  
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            </p>
 
  
  
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        </p>
 
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        <p>
 
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        </p>
 
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        <table style="width:100%">
 
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<thead>
 
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<td align='center'>Species sign in ODE system</td>
 
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<td align='center'>Species</td>
 
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<td align='center'>Initial concentration (M)</td>
 
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</thead>
 
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<tbody>
 
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<tr>
 
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<td align='center'>A</td>
 
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<td align='center'>pDNA+RNA I+RNAII early</td>
 
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<td align='center'>0</td>
 
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</tr>
 
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<tr>
 
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<td align='center'>B</td>
 
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<td align='center'>pDNA+RNA II short</td>
 
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<td align='center'>0</td>
 
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</tr>
 
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<tr>
 
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<td align='center'>RNAI</td>
 
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<td align='center'>RNA I</td>
 
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<td align='center'>1E-6</td>
 
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</tr>
 
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<tr>
 
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<td align='center'>D</td>
 
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<td align='center'>pDNA+RNA II long</td>
 
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<td align='center'>0</td>
 
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</tr>
 
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<tr>
 
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<td align='center'>E</td>
 
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<td align='center'>pDNA+RNAII primer</td>
 
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<td align='center'>0</td>
 
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</tr>
 
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<tr>
 
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<td align='center'>F</td>
 
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<td align='center'>RNA II long</td>
 
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<td align='center'>0</td>
 
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</tr>
 
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<tr>
 
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<td align='center'>G</td>
 
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<td align='center'>pDNA</td>
 
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<td align='center'>4E-8*</td>
 
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</tr>
 
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<tr>
 
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<td align='center'>H</td>
 
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<td align='center'>pDNA+RNA II+RNA I late</td>
 
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<td align='center'>0</td>
 
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</tr>
 
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<tr>
 
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<td align='center'>RNA II</td>
 
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<td align='center'>RNA II</td>
 
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<td align='center'>0</td>
 
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</tr>
 
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<tr>
 
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<td align='center'>J</td>
 
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<td align='center'>RNAI+RNAII</td>
 
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<td align='center'>0</td>
 
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</tr>
 
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</tbody>
 
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</table>
 
 
     </div>
 
     </div>
 
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</div>

Revision as of 16:29, 17 October 2018

Human Practices Overview

Reaching Society

Encouraged by the rapid Life Sciences development in Lithuania and our team’s previous achievements, we decided that it was meaningful to share our experience with the younger generation. Visiting schools, inviting pupils to visit Vilnius University Life Sciences Center and introducing SynDrop to them helped us not only to reveal but also clarify our own attitude towards synthetic biology. The discussion that we have organised during the DNA Day’s celebration has become a great inspiration to search for a creative approach to implement our project’s idea and to make it more public-friendly.

Passing on the Knowledge to the Younger Generation

Excited by the possibilities synthetic biology has to offer, our team aimed to involve younger generation in this novel field of science by educating them in schools, during pupil-olympiads, and meetings of other societies.

Our team members continued the tradition of previous Vilnius-Lithuania iGEM teams and held lectures for students in different schools across the country. During these visits scholars had an opportunity to learn the main principles of synthetic biology and laboratory work bearing in mind SynDrop project as an example. Also, high-school students have visited our laboratory in Vilnius University Life Sciences Center and implemented their knowledge into practice, e.g. learning how to balance a centrifuge or using Burker camera in order to calculate cells.

Additionally, we delegated our member Valentas to represent our team in organising the 51st LitBO (Lithuanian Biology Olympiad) and its bootcamp where students were preparing for the International Biology Olympiad. He was responsible for giving lectures about fundamental biological principles and synthetic biology, as well as planning experiments for students.

Our instructor Auksė held a lecture for Turing School students (13-18 yrs. of age) who planned to become future inventors and leaders of the IT sector. She delineated the possibilities of synthetic biology, revealed future applications of CRISPR/Cas system, and most importantly described ways of how IT skills could be applied in bioinformatics and above mentioned fields.

>

SILVER photo

DNA Day's Celebration

invert