Difference between revisions of "Team:WashU StLouis"

 
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{{WashU_StLouis}}
 
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<h1> A Detection Mechanism for Wheat Rust Fungus </h1>
 
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<p>Washington University in St. Louis iGEM Project 2018 </p>
 
  
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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      <a href="https://2018.igem.org/Team:WashU_StLouis/Overview" title="Project Overview">
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    <div class="cont"><p style="font-size:32px;line-height:34px;font-family: 'Josefin Sans';">OUR NOVEL MULTISTEP APPROACH</p></div>
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<div class="column full_size" >
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    <div class="cont">
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<h3> Styling your wiki </h3>
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      <p style="font-size:14px;font-family: 'Josefin Sans'">
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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Virulent races of Puccinia graminis f. sp. tritici (Pgt), or wheat stem rust, have caused devastating effects on cereal grains worldwide, impacting global food security. We are engineering <I>Escherichia coli DH5α</I> and <I>Saccharomyces cerevisiae EBY100</I> to detect Pgt and improve response times to virulent strains. To detect Pgt, we are creating a device that will germinate spores from the Puccinia genus. The germinated spores produce ribitol, a sugar unique to Pgt. Our engineered <I>DH5α</I> will produce a fluorescent signal in the presence of ribitol, thus detecting Pgt. To detect specific virulent races of Pgt, we will modify yeast to contain the stem rust resistance gene Sr35 from Triticum monococcum; Sr35 recognizes its corresponding effector AvrSr35, secreted by Pgt, as part of the plant’s innate immune system. Using bimolecular fluorescence complementation, our yeast will detect AvrSr35, a first step in being able to indicate the virulence of the germinated Pgt spores.
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<p style="font-size:26px;font-family: 'Josefin Sans'">Transform <I>Escherichia coli DH5α</I> with a plasmid carrying a promoter induced by ribitol, a compound unique to infectious structures formed by fungal spores, upstream of a fluorescent protein.</p>
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      </div>
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    <div id="dsdesc" class="spdesc">
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      <div class="cont">
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<p style="font-size:26px;font-family: 'Josefin Sans'">Express wheat resistance genes in transformed <I>Saccharomyces cerevisiae EBY100</I> to detect virulence factors released by Pgt to distinguish whether or not the race of Pgt present is virulent to the strain of wheat.</p>
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      </a>
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    <a href="https://2018.igem.org/Team:WashU_StLouis/Broad" title="Detect">
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    Detect
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      </a>
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      <a href="https://2018.igem.org/Team:WashU_StLouis/Avirulence" title="Profile">
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    Profile
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<a href="https://2018.igem.org/Team:WashU_StLouis/Human_Practices" title="Human Practices">
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  <center><img src="https://static.igem.org/mediawiki/2018/0/08/T--WashU_StLouis--humanpracticesicon.png" height="64px"/></center>
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<a href="https://2018.igem.org/Team:WashU_StLouis/Demonstrate" title="Demonstrate">
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<a href="https://2018.igem.org/Team:WashU_StLouis/Composite_Part" title="Parts">
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      <div class="subpanel8" style="margin-right:0">
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<a href="https://2018.igem.org/Team:WashU_StLouis/Collaborations" title="Collaborations">
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  <center><img src="https://static.igem.org/mediawiki/2018/8/8e/T--WashU_StLouis--collaborationicon.png" height="64px"/></center>
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<h3> Uploading pictures and files </h3>
 
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
 
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
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<h3> Wiki template information </h3>
 
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
 
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<a href="https://2018.igem.org/wiki/index.php?title=Team:WashU_StLouis&action=edit">
 
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EDIT PAGE
 
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
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<ul>
 
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
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<li>Be clear about what you are doing and how you plan to do this.</li>
 
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
 
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<li>Have lots of fun! </li>
 
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
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                <a href="https://2018.igem.org/Team:WashU/Team">Team</a>
 
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                    <li><a href="https://2018.igem.org/Team:WashU/Team">Team</a></li>
 
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                    <li><a href="https://2018.igem.org/Team:WashU/Team/Financing">Financing</a></li>
 
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                    <li><a href="https://2018.igem.org/Team:WashU/Team/Collaborations">Collaborations</a></li>
 
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Latest revision as of 16:32, 17 October 2018

OUR NOVEL MULTISTEP APPROACH

Virulent races of Puccinia graminis f. sp. tritici (Pgt), or wheat stem rust, have caused devastating effects on cereal grains worldwide, impacting global food security. We are engineering Escherichia coli DH5α and Saccharomyces cerevisiae EBY100 to detect Pgt and improve response times to virulent strains. To detect Pgt, we are creating a device that will germinate spores from the Puccinia genus. The germinated spores produce ribitol, a sugar unique to Pgt. Our engineered DH5α will produce a fluorescent signal in the presence of ribitol, thus detecting Pgt. To detect specific virulent races of Pgt, we will modify yeast to contain the stem rust resistance gene Sr35 from Triticum monococcum; Sr35 recognizes its corresponding effector AvrSr35, secreted by Pgt, as part of the plant’s innate immune system. Using bimolecular fluorescence complementation, our yeast will detect AvrSr35, a first step in being able to indicate the virulence of the germinated Pgt spores.

Transform Escherichia coli DH5α with a plasmid carrying a promoter induced by ribitol, a compound unique to infectious structures formed by fungal spores, upstream of a fluorescent protein.

Express wheat resistance genes in transformed Saccharomyces cerevisiae EBY100 to detect virulence factors released by Pgt to distinguish whether or not the race of Pgt present is virulent to the strain of wheat.
Detect
Profile