Difference between revisions of "Team:NEU China B/Collaborations"

Collaboration with DUOT

Our two teams have held a meeting to discuss some question about the whole project.
During the meeting, we solved some problems of our projects.

Question from NEU

Quiz1：The idea of your team is very novel and has important practical value. But as a medicine that enters the human body, how to control it does not harm the intestinal flora?
Answer: We have reviewed a group quantity control system of Imperial College of Science and Technology in 2016. We have made improvements in light of our own situation. Simply put, the concentration of signal substances controlled by the bacteria itself controls the opening and closing of the suicide system, thereby regulating the number of bacteria.
Quiz2：Your final product is a bacterial preparation. There are great potential threats to the environment. Have you thought about any suicide mechanism? We are doing a cold lethal system, and if you are interested we can cooperate.
Answer：Thank you very much for your proposal. We also checked the information and found the Blu-ray lethal system, etc., but I am still very happy to work with you. You can provide our plasmids. We can help you to characterize and provide relevant data.

Question from DUOT

Quiz1: As a system for detecting lactic acid, what are the advantages of your products with lactic acid detection products on the market?
Answer: Compared with the normal detection of lactic acid products, we have more use of biological related technologies. By referring to some of the design experience of the 2015-year team at the ETH Zurich, we used the group sensing system of E. coli and used fiber optic circuitry for detection,. It is low cost and ease of use.

Quiz2: How do you ensure that E. coli in your system does not pollute the fermentation industry? Answer: We intend to paste our product into a portable device, and the part that touches the fermentation liquid is only the probe. The E. coli inside the unit does not pollute the fermentation broth.

Collaboration with SCAU-China

Our team conducted an exchange discussion with NEU_China_B in August 2018. This meeting is mainly to share the two team's projects main content and two teams each encountered in the experiment problem. The Q&A are as follow.
NEU_China_B: How do you ensure that the integrative vector in transformants will not lose?
SCAU-China: The integrative vector we used is pFQ20 provided by the Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences. The homology arms in integrative vector for homologous recombination allows the target genes to be stably transferred into the genome of Microcoleus vaginatus. It has been verified through our experiment results.
SCAU-China: How to match the concentration of AI-2 expressed by the first plasmid pCFDuet-1 with the GFP concentration expressed by pet28a(+).

NEU_China_B: Our experiment was to release the AI-2 signal molecule after the pCFDuet-1 expression plasmid induction of lactic acid concentration, and AI-2 signal molecule can inhibit the lldr sequence on the second plasmid pet28a(+).
The lldr sequence is capable of producing GFP-deterring proteins. In the experiment, we verified that two plasmid copies were more similar, and that the o1o2 sequence could be started normally when the lactic acid concentration was lower (less than 1m mol), and then the GFP gene could be produced fluorescent signal.
Therefore, the expression of two plasmids is more matched, and the experimental results are not affected in this project.

What’s more, we provided the yogurt produced by SCAU Assets Management Co.Ltd. to NEU_China_B as the experiment materials for their LED project, and they obtained considerable data in the detection of lactic acid content.

Collaboration with Fujian normal university

1. Online meeting.

Our two teams focused on the problems encountered in the experiment, including some enzymes that we did not use like Gibson enzymes. Our team mainly raised the problem of the actual operation of importing two plasmids into bacteria. In response to this problem, the students of Fujian Normal University gave us a solution. They tell us that we need to import the first plasmid and then the second plasmid. In addition, they also provide us with some experience about fermentation industry.

2. Plasmid construction

We have two teams in the construction of the plasmid also carried out a certain level of cooperation. The students of Fujian Normal University helped us to construct plasmid p-LsrA-GFP. We helped them to measure the fluorescence value of proU-GFP and construct the plasmid proU-GFP. (http://parts.igem.org/Part:BBa_K2570020)

3. Visiting beer factory

Under the guidance of the students of Fujian Normal University, we have visited the beer factory inside the college to learn about the whole production line of beer production and the function of each step. In the course of the visit, we retrieved a portion of the beer fermentation liquid to observe the experiment, and used chemical methods to detect the lactic acid content in the fermentation process.

(photos of fermentation tanks in FJNU)

4. Handbook

We help the students of Fujian Normal University make a handbook about the strains. This note is intended primarily to understand the perceptions of different strains in people around the world. On top of this we plan to carry out the co-operation of the part of both teams.