Difference between revisions of "Team:OUC-China/Experiments"
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This year, our project can be divided into four systems. Between the different systems, there are different protocols. And we have sorted out these experimentals in detail. | This year, our project can be divided into four systems. Between the different systems, there are different protocols. And we have sorted out these experimentals in detail. | ||
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| − | <div class="chapai"> | + | <div class="chapai"><br /> |
| − | <h4 ><font size=" | + | <h4 ><font size="6">1.Elementary Operation</font></h4 > |
| − | <br />Conservation | + | <div style="text-align: left;"> |
| − | <br />Gel Electrophoresis | + | <br />  （1）<a href="https://static.igem.org/mediawiki/2018/d/d2/T--OUC-China--conserva.pdf">Conservation</a> |
| − | <br />Gel Extraction | + | <br />  （2）<a href="https://static.igem.org/mediawiki/2018/9/9d/T--OUC-China--gel.pdf">Gel Electrophoresis</a> |
| − | <br />Inoculation | + | <br />  （3）<a href="https://static.igem.org/mediawiki/2018/e/e2/T--OUC-China--gel2.pdf">Gel Extraction </a> |
| − | <br />Ligation system | + | <br />  （4）<a href="https://static.igem.org/mediawiki/2018/1/12/T--OUC-China--ino.pdf">Inoculation</a> |
| − | <br />medium | + | <br />  （5）<a href="https://static.igem.org/mediawiki/2018/f/fe/T--OUC-China--liga.pdf">Ligation system</a> |
| − | <br />Plasmid extraction | + | <br />  （6）<a href="https://static.igem.org/mediawiki/2018/1/11/T--OUC-China--medium.pdf">medium</a> |
| − | <br />Polymerase Chain Reaction | + | <br />  （7）<a href="https://static.igem.org/mediawiki/2018/3/35/T--OUC-China--plas1.pdf">Plasmid extraction</a> |
| − | <br />Restriction Enzyme Digestion | + | <br />  （8）<a href="https://static.igem.org/mediawiki/2018/d/d2/T--OUC-China--poly.pdf">Polymerase Chain Reaction</a> |
| − | <br />Sterilization | + | <br />  （9）<a href="https://static.igem.org/mediawiki/2018/6/6e/T--OUC-China--res.pdf">Restriction Enzyme Digestion </a> |
| − | <br />Transformation of Escherichia coli | + | <br />  （10）<a href="https://static.igem.org/mediawiki/2018/9/98/T--OUC-China--ster.pdf">Sterilization</a> |
| − | <h4 ><font size=" | + | <br />  （11）<a href="https://static.igem.org/mediawiki/2018/8/86/T--OUC-China--transr.pdf">Transformation of Escherichia coli</a> </div> <br /><br /> |
| − | <br />Assay of Gene Expression by Flow Cytometry | + | <h4 ><font size="6">2.Systems Detection</font></h4 > |
| − | <br />Fluorescence measurement | + | <div style="text-align: left;"> |
| − | <br />miniToe Polycistron Fluorescence measurement | + | <br />  （1）<a href="https://static.igem.org/mediawiki/2018/6/61/T--OUC-China--assay.pdf">Assay of Gene Expression by Flow Cytometry</a> |
| − | <br />The application of miniToe——Regulation of motA | + | <br />  （2）<a href="https://static.igem.org/mediawiki/2018/b/b1/T--OUC-China--fluo.pdf">Fluorescence measurement</a> |
| − | + | <br />  （3）<a href="https://static.igem.org/mediawiki/2018/d/dd/T--OUC-China--minitoe.pdf">miniToe Polycistron Fluorescence measurement</a> | |
| − | + | <br />  （4）<a href="https://static.igem.org/mediawiki/2018/f/f6/T--OUC-China--thepp.pdf">The application of miniToe——Regulation of motA </a> </div> | |
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| − | <div class="copyright1">Contact Us : oucigem@163.com | ©2018 OUC IGEM.All Rights Reserved. | + | <div class="copyright1">Contact Us : oucigem@163.com | ©2018 OUC IGEM.All Rights Reserved. <br /> |
| + | <img src="https://static.igem.org/mediawiki/2017/b/b4/T--OUC-China--foot1.jpeg"alt="banner"width="80px"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/6/62/T--OUC-China--foot2.jpeg"alt="banner"width="80px"> | ||
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Latest revision as of 17:18, 17 October 2018
Experiments
This year, our project can be divided into four systems. Between the different systems, there are different protocols. And we have sorted out these experimentals in detail.
1.Elementary Operation
（1）Conservation
（2）Gel Electrophoresis
（3）Gel Extraction
（4）Inoculation
（5）Ligation system
（6）medium
（7）Plasmid extraction
（8）Polymerase Chain Reaction
（9）Restriction Enzyme Digestion
（10）Sterilization
（11）Transformation of Escherichia coli
2.Systems Detection
（1）Assay of Gene Expression by Flow Cytometry
（2）Fluorescence measurement
（3）miniToe Polycistron Fluorescence measurement
（4）The application of miniToe——Regulation of motA
Contact Us : oucigem@163.com | ©2018 OUC IGEM.All Rights Reserved.
