Difference between revisions of "Team:OUC-China/Experiments"

 
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<h4 ><font size="6">1.Elementary Operation</font></h4 >
 
<h4 ><font size="6">1.Elementary Operation</font></h4 >
 
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<div style="text-align: left;">
<br />(1)Conservation
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<br />&emsp;&emsp;(1)<a href="https://static.igem.org/mediawiki/2018/d/d2/T--OUC-China--conserva.pdf">Conservation</a>
<br />(2)Gel Electrophoresis
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<br />&emsp;&emsp;(2)<a href="https://static.igem.org/mediawiki/2018/9/9d/T--OUC-China--gel.pdf">Gel Electrophoresis</a>
<br />(3)Gel Extraction
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<br />&emsp;&emsp;(3)<a href="https://static.igem.org/mediawiki/2018/e/e2/T--OUC-China--gel2.pdf">Gel Extraction </a>
<br />(4)Inoculation
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<br />&emsp;&emsp;(4)<a href="https://static.igem.org/mediawiki/2018/1/12/T--OUC-China--ino.pdf">Inoculation</a>
<br />(5)Ligation system
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<br />&emsp;&emsp;(5)<a href="https://static.igem.org/mediawiki/2018/f/fe/T--OUC-China--liga.pdf">Ligation system</a>
<br />(6)medium
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<br />&emsp;&emsp;(6)<a href="https://static.igem.org/mediawiki/2018/1/11/T--OUC-China--medium.pdf">medium</a>
<br />(7)Plasmid extraction
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<br />&emsp;&emsp;(7)<a href="https://static.igem.org/mediawiki/2018/3/35/T--OUC-China--plas1.pdf">Plasmid extraction</a>
<br />(8)Polymerase Chain Reaction
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<br />&emsp;&emsp;(8)<a href="https://static.igem.org/mediawiki/2018/d/d2/T--OUC-China--poly.pdf">Polymerase Chain Reaction</a>
<br />(9)Restriction Enzyme Digestion
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<br />&emsp;&emsp;(9)<a href="https://static.igem.org/mediawiki/2018/6/6e/T--OUC-China--res.pdf">Restriction Enzyme Digestion </a>
<br />(10)Sterilization
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<br />&emsp;&emsp;(10)<a href="https://static.igem.org/mediawiki/2018/9/98/T--OUC-China--ster.pdf">Sterilization</a>
<br />(11)Transformation of Escherichia coli </div> <br /><br />
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<br />&emsp;&emsp;(11)<a href="https://static.igem.org/mediawiki/2018/8/86/T--OUC-China--transr.pdf">Transformation of Escherichia coli</a> </div> <br /><br />
<h4 ><font size="6">2.Systems Detection</font></h4 >
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<h4 ><font size="6">2.Systems Detection</font></h4 >
<br />(1)Assay of Gene Expression by Flow Cytometry
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<div style="text-align: left;">
<br />(2)Fluorescence measurement
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<br />&emsp;&emsp;(1)<a href="https://static.igem.org/mediawiki/2018/6/61/T--OUC-China--assay.pdf">Assay of Gene Expression by Flow Cytometry</a>
<br />(3)miniToe Polycistron Fluorescence measurement
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<br />&emsp;&emsp;(2)<a href="https://static.igem.org/mediawiki/2018/b/b1/T--OUC-China--fluo.pdf">Fluorescence measurement</a>
<br />(4)The application of miniToe——Regulation of motA
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<br />&emsp;&emsp;(3)<a href="https://static.igem.org/mediawiki/2018/d/dd/T--OUC-China--minitoe.pdf">miniToe Polycistron Fluorescence measurement</a>
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<br />&emsp;&emsp;(4)<a href="https://static.igem.org/mediawiki/2018/f/f6/T--OUC-China--thepp.pdf">The application of miniToe——Regulation of motA </a>  </div>
 
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</section>
 
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   <div class="copyright1">Contact Us : oucigem@163.com  | &copy;2018 OUC IGEM.All Rights Reserved.  |  ………… </div>
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   <div class="copyright1">Contact Us : oucigem@163.com  | &copy;2018 OUC IGEM.All Rights Reserved. <br />
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Latest revision as of 17:18, 17 October 2018

Team OUC-China: Main

Experiments

This year, our project can be divided into four systems. Between the different systems, there are different protocols. And we have sorted out these experimentals in detail.  


1.Elementary Operation


  (1)Conservation
  (2)Gel Electrophoresis
  (3)Gel Extraction
  (4)Inoculation
  (5)Ligation system
  (6)medium
  (7)Plasmid extraction
  (8)Polymerase Chain Reaction
  (9)Restriction Enzyme Digestion
  (10)Sterilization
  (11)Transformation of Escherichia coli


2.Systems Detection








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