Difference between revisions of "Team:OUC-China/Experiments"

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<h1>Experiments</h1>
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<p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
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Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.  
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<ul>
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<li ><a href="https://2018.igem.org/Team:OUC-China">Main</a>
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</li>
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<li>
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<a href="#">Project</a>
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<ul>
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<li><a href="https://2018.igem.org/Team:OUC-China/Description">Overview</a></li>
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<li><a href="https://2018.igem.org/Team:OUC-China/Design">Design</a></li>
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<li><a href="https://2018.igem.org/Team:OUC-China/Results">Results</a></li>
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<li><a href="https://2018.igem.org/Team:OUC-China/Demonstrate">Demonstrate</a></li>
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</ul>
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</li>
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<li><a href="#">Lab WORK</a>
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<ul>
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<li><a href="https://2018.igem.org/Team:OUC-China/Experiments">Experiments</a></li>
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<li><a href="https://2018.igem.org/Team:OUC-China/Safety">Safety</a></li>
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<li><a href="https://2018.igem.org/Team:OUC-China/InterLab">InterLab</a></li>
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</ul>
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</li>
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<li><a href="#">Model</a>
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                                                <ul>
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<li><a href="https://2018.igem.org/Team:OUC-China/Model">Overview</a></li>
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<li><a href="https://2018.igem.org/Team:OUC-China/miniToe">miniToe</a></li>
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<li><a href="https://2018.igem.org/Team:OUC-China/miniToe Family">miniToe Family</a></li>
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<li><a href="https://2018.igem.org/Team:OUC-China/polycistron">polycistron</a></li>
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</ul>
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</li>
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  <li><a href="#">Parts</a>
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<ul>
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<li><a href="https://2018.igem.org/Team:OUC-China/Parts">List</a></li>
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<li><a href="https://2018.igem.org/Team:OUC-China/Improve">Improve</a></li>
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</ul>
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<li><a href="#">HP</a>
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<ul>
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<li><a href="https://2018.igem.org/Team:OUC-China/Human_Practices">Human Practices</a></li>
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<li><a href="https://2018.igem.org/Team:OUC-China/Public_Engagement">Public Engagement</a></li>
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</ul>
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<li><a href="#">Team</a>
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<ul>
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<li><a href="https://2018.igem.org/Team:OUC-China/Team">Team Member</a></li>
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<li><a href="https://2018.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li>
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<li><a href="https://2018.igem.org/Team:OUC-China/Attributions">Attributions</a></li>
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</ul>
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</li>
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<li><a href="https://2018.igem.org/Team:OUC-China/Notebook">Notebook</a></li>
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<!-- Features -->
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<h2 class="major"><span>Experiments</span></h2>
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<p align="justify">
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<h3></h3>
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This year, our project can be divided into four systems. Between the different systems, there are different protocols. And we have sorted out these experimentals in detail.
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<h4 ><font size="6">1.Elementary Operation</font></h4 >
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<div style="text-align: left;">
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<br />&emsp;&emsp;(1)<a href="https://static.igem.org/mediawiki/2018/d/d2/T--OUC-China--conserva.pdf">Conservation</a>
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<br />&emsp;&emsp;(2)<a href="https://static.igem.org/mediawiki/2018/9/9d/T--OUC-China--gel.pdf">Gel Electrophoresis</a>
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<br />&emsp;&emsp;(3)<a href="https://static.igem.org/mediawiki/2018/e/e2/T--OUC-China--gel2.pdf">Gel Extraction </a>
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<br />&emsp;&emsp;(4)<a href="https://static.igem.org/mediawiki/2018/1/12/T--OUC-China--ino.pdf">Inoculation</a>
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<br />&emsp;&emsp;(5)<a href="https://static.igem.org/mediawiki/2018/f/fe/T--OUC-China--liga.pdf">Ligation system</a>
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<br />&emsp;&emsp;(6)<a href="https://static.igem.org/mediawiki/2018/1/11/T--OUC-China--medium.pdf">medium</a>
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<br />&emsp;&emsp;(7)<a href="https://static.igem.org/mediawiki/2018/3/35/T--OUC-China--plas1.pdf">Plasmid extraction</a>
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<br />&emsp;&emsp;(8)<a href="https://static.igem.org/mediawiki/2018/d/d2/T--OUC-China--poly.pdf">Polymerase Chain Reaction</a>
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<br />&emsp;&emsp;(9)<a href="https://static.igem.org/mediawiki/2018/6/6e/T--OUC-China--res.pdf">Restriction Enzyme Digestion </a>
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<br />&emsp;&emsp;(10)<a href="https://static.igem.org/mediawiki/2018/9/98/T--OUC-China--ster.pdf">Sterilization</a>
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<br />&emsp;&emsp;(11)<a href="https://static.igem.org/mediawiki/2018/8/86/T--OUC-China--transr.pdf">Transformation of Escherichia coli</a> </div> <br /><br />
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<h4 ><font size="6">2.Systems Detection</font></h4 >
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<div style="text-align: left;">
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<br />&emsp;&emsp;(1)<a href="https://static.igem.org/mediawiki/2018/6/61/T--OUC-China--assay.pdf">Assay of Gene Expression by Flow Cytometry</a>
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<br />&emsp;&emsp;(2)<a href="https://static.igem.org/mediawiki/2018/b/b1/T--OUC-China--fluo.pdf">Fluorescence measurement</a>
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<br />&emsp;&emsp;(3)<a href="https://static.igem.org/mediawiki/2018/d/dd/T--OUC-China--minitoe.pdf">miniToe Polycistron Fluorescence measurement</a>
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<br />&emsp;&emsp;(4)<a href="https://static.igem.org/mediawiki/2018/f/f6/T--OUC-China--thepp.pdf">The application of miniToe——Regulation of motA </a>  </div>
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<h3>What should this page contain?</h3>
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<ul>
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<li> Protocols </li>
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<li> Experiments </li>
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<li> Documentation of the development of your project </li>
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</ul>
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<h3>Inspiration</h3>
 
<ul>
 
<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
 
<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
 
<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
 
</ul>
 
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  <div class="copyright1">Contact Us : oucigem@163.com  | &copy;2018 OUC IGEM.All Rights Reserved. <br />
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Latest revision as of 17:18, 17 October 2018

Team OUC-China: Main

Experiments

This year, our project can be divided into four systems. Between the different systems, there are different protocols. And we have sorted out these experimentals in detail.  


1.Elementary Operation


  (1)Conservation
  (2)Gel Electrophoresis
  (3)Gel Extraction
  (4)Inoculation
  (5)Ligation system
  (6)medium
  (7)Plasmid extraction
  (8)Polymerase Chain Reaction
  (9)Restriction Enzyme Digestion
  (10)Sterilization
  (11)Transformation of Escherichia coli


2.Systems Detection








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