Difference between revisions of "Team:OUC-China/Safety"

 
(18 intermediate revisions by 2 users not shown)
Line 38: Line 38:
 
}
 
}
  
−
 
+
 +
.nb{
 +
background-color:#F7F7F7;
 +
}
 +
 +
 +
.sk{
 +
width:1000px;
 +
padding:0px 50px 0px 50px;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
background-color:white;
 +
}
 +
  
 
#myBtn {
 
#myBtn {
Line 141: Line 154:
 
<li><a href="#">Team</a>
 
<li><a href="#">Team</a>
 
<ul>
 
<ul>
−
<li><a href="https://2018.igem.org/Team:OUC-China/Team">Team_Member</a></li>
+
<li><a href="https://2018.igem.org/Team:OUC-China/Team">Team Member</a></li>
 
<li><a href="https://2018.igem.org/Team:OUC-
 
<li><a href="https://2018.igem.org/Team:OUC-
  
Line 165: Line 178:
 
</div>
 
</div>
 
<!-- Main -->
 
<!-- Main -->
−
<div id="main" class="container">
+
<div class="nb">
 
 
 
 
 
 
−
<div class="12u">
+
<div class="sk">
  
 
<!-- Features -->
 
<!-- Features -->
Line 179: Line 192:
 
<p>
 
<p>
 
<h3></h3>
 
<h3></h3>
−
&emsp;&emsp;Safety is the acutest problem when it comes to genetically modified organism. We  
+
Safety is the acutest problem when it comes to genetically modified organism. We figured this problem out from three
  
−
figured this problem out from three aspects: Project design, Lab work and Shipment.
+
aspects: Project design, Lab work and Shipment.
 
<br /><br />
 
<br /><br />
 
<h3>Project design</h3>
 
<h3>Project design</h3>
−
<br />&emsp;&emsp;Being responsible for our earth and ourselves is our attitude towards every  
+
<br />Being responsible for our earth and ourselves is our attitude towards every  
  
 
experiment. In our project, all the parts we have utilized are selected from Risk Group 1, none of  
 
experiment. In our project, all the parts we have utilized are selected from Risk Group 1, none of  
−
 
+
−
which are with a Red Flag. What’s more, nonpathogenic bacteria, E.coli DH5α is employed as the main  
+
which are with a Red Flag. What’s more, nonpathogenic bacteria, <i>E.coli</i> DH5 Alpha is employed as the main  
  
 
chassis for our prior design on the DNA sequence, stem-loop and enzyme. As a matter of fact, when  
 
chassis for our prior design on the DNA sequence, stem-loop and enzyme. As a matter of fact, when  
Line 196: Line 209:
 
industrialized applications and scientific research. Based on this, We designed more variants of csy4  
 
industrialized applications and scientific research. Based on this, We designed more variants of csy4  
  
−
enzymes and hairpins. (See more details in xxx and xxx about the variants of csy4 enzymes and
+
enzymes and hairpins. （Click <a href='https://2018.igem.org/Team:OUC-China/Design'> here </a> to see more! ）So we used these to create a toolkit named miniToe family for synthetic biology which can  
−
 
+
−
hairpins.）So we used these to create a toolkit named miniToe family for synthetic biology which can  
+
  
 
control the expression of the target gene gradient. What’s more, we used the motA gene, one of the  
 
control the expression of the target gene gradient. What’s more, we used the motA gene, one of the  
  
−
genes regulating the motility of E.coli, to verify the feasibility and effectiveness of the toolkit.  
+
genes regulating the motility of <i>E.coli</i>, to verify the feasibility and effectiveness of the toolkit.  
  
 
For the sake of environment, all the chassis organisms will be sterilized before being abandoned to  
 
For the sake of environment, all the chassis organisms will be sterilized before being abandoned to  
Line 210: Line 221:
 
<p>
 
<p>
 
<h3>Safe lab work</h3>
 
<h3>Safe lab work</h3>
−
<br />&emsp;&emsp;We works at a neat lab named “Lab for Microinnovation and Enterprise”, which is a  
+
<br />We works at a neat lab named “Lab for Microinnovation and Enterprise”, which is a  
  
 
BSL-2 laboratory. Before working in the lab, we are supposed to be trained by the skillful hands, not  
 
BSL-2 laboratory. Before working in the lab, we are supposed to be trained by the skillful hands, not  
Line 235: Line 246:
 
precautions</p></div>  
 
precautions</p></div>  
  
−
&emsp;&emsp;Then for lab safety, a routine inspection is performed twice a day by everyone, not only  
+
Then for lab safety, a routine inspection is performed twice a day by everyone, not only for ensuring normal operation
  
−
for ensuring normal operation of our lab like supplying experimental consumables and inspecting the  
+
of our lab like supplying experimental consumables and inspecting the running of instruments, but also keeps it away
  
−
running of instruments, but also keeps it away from non-biological hazards, especially the water and  
+
from non-biological hazards, especially the water and electricity. For high efficiency, responsibility implements to
  
−
electricity. For high efficiency, responsibility implements to every team member.
+
every team member.
 
<br />
 
<br />
 
<br />
 
<br />
Line 251: Line 262:
 
width="400" >
 
width="400" >
 
</div>  
 
</div>  
−
<br />
+
<br /> <div align="center"><p style="font-size:80%;color:gray"">Fig.2 Table for routine inspection</p></div>  
−
<div align="center"><p style="font-size:80%;color:gray"">Fig.2 Table for routine inspection</p></div>  
+
Furthermore, several operational zones are divided scientifically for special experiments like A zone where is weighing
  
−
<br />&emsp;&emsp;Furthermore, several operational zones are divided scientifically for special
+
the drugs, preparing the medium and sterilizing, B zone where is the recording experimental note and organizing
  
−
experiments like A zone where is weighing the drugs, preparing the medium and sterilizing, B zone  
+
experimental data area, C zone where is a molecular operation area, D zone where is RNA electrophoresis and a
  
−
where is the recording experimental note and organizing experimental data area, C zone where is a  
+
polyacrylamide gel electrophoresis area. For us, lab safety is a hot topic and we have come up with a practical lab
  
−
molecular operation area, D zone where is RNA electrophoresis and a polyacrylamide gel electrophoresis
+
safety manual which normalizes our
  
−
area.
+
lovely lab.
−
<br />&emsp;&emsp;For us, lab safety is a hot topic and we have come up with a practical lab safety
+
−
 
+
−
manual which normalizes our lovely lab.
+
 
  <br />
 
  <br />
 
  <br />
 
  <br />
 
<h3>Practical lab safety manual</h3>
 
<h3>Practical lab safety manual</h3>
−
<br />1.Routine inspections must perform between 6:30-8:00pm and 9:30-11:00pm.
+
<br /> 1.Routine inspections must perform between 6:30-8:00pm and 9:30-11:00pm.
−
<br />2.Lab cleaning must be done twice a week with disinfectant moping the floor for at least 3times.
+
<br /> 2.Lab cleaning must be done twice a week with disinfectant moping the floor for at least 3times.
−
<br />3.Well prepared experiment plans should be written before you start.
+
<br /> 3.Well prepared experiment plans should be written before you start.
−
<br />4.Lab coats and gloves must be worn when conducting experiments.
+
<br /> 4.Lab coats and gloves must be worn when conducting experiments.
−
<br />5.Short skirts, shorts, and open shoes must not be worn.
+
<br /> 5.Short skirts, shorts, and open shoes must not be worn.
−
<br />6.If you see a colleague doing something dangerous, point it out to him or her.
+
<br /> 6.If you see a colleague doing something dangerous, point it out to him or her.
−
<br />7.Lab coats must not be worn outside laboratories and in public areas.
+
<br /> 7.Lab coats must not be worn outside laboratories and in public areas.
−
<br />8.Know where safety equipment (eyewash, shower and extinguisher) is located.
+
<br /> 8.Know where safety equipment (eyewash, shower and extinguisher) is located.
−
<br />9.Food/drink is not allowed in laboratories where chemicals are used stored.
+
<br /> 9.Food/drink is not allowed in laboratories where chemicals are used stored.
−
<br />10.Know how to clean up spills of common chemicals and specific chemicals you see, for spilling
+
<br /> 10.Always read the directions before using instruments and read MSDS before handling new  
−
 
+
−
ethidium bromide dealt with sodium hydroxide.
+
−
<br />11.Always read the directions before using instruments and read MSDS before handling new  
+
  
 
chemicals carefully.
 
chemicals carefully.
−
<br />12.When post-stain gel electrophoresis, we must wear the gloves that are on the left two and
 
−
 
−
right three.
 
 
<br /> <br />
 
<br /> <br />
 
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/3/38/T--OUC-China--Safety004.jpg"  
 
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/3/38/T--OUC-China--Safety004.jpg"  
Line 295: Line 297:
 
height="400" >
 
height="400" >
 
</div>  <br />             
 
</div>  <br />             
−
  <div class="pos_top ">
+
  <div align="center">
 
  <img src="https://static.igem.org/mediawiki/2018/d/d1/T--OUC-China--Safety005.jpg" width="840" >
 
  <img src="https://static.igem.org/mediawiki/2018/d/d1/T--OUC-China--Safety005.jpg" width="840" >
 
</div>  
 
</div>  
−
<div align="center">
+
<br /> <div align="center"><p style="font-size:80%;color:gray"">Fig.3 Safety operation</p></div>  
−
<br />&emsp;&emsp;
+
  
−
<br />&emsp;&emsp;
+
<h3>Safe shipment</h3>
 +
<br />For submitting our parts to igem HQ, we first took the initiative to consult Chinese entry-exit inspection and quarantine laws and regulations about shipping DNA from China to the USA, which is forbidden without tedious red tape. Fortunately, GenScript cooperated with igem HQ forward our part sample to the USA. Ergo, we sealed dried DNA and sent it in the submission kit to GenScript, which clearly labelled as DNA without disguise. Our kit poses no risks and is perfectly safe for shipment.
  
−
<br />&emsp;&emsp;
+
<br />
−
 
+
<br />
−
<br />&emsp;&emsp;
+
<h3>Safety form</h3>
 +
<br />  <a href="https://2018.igem.org/Safety/Final_Safety_Form?team_id=2615">Click here to check our safety form.</a>
 +
<br /><br /><br /><br /><br /><br />
  
 
</p>
 
</p>
Line 337: Line 341:
  
  
−
  <div class="copyright1">Contact Us : oucigem@163.com  | &copy;2018 OUC IGEM.All Rights Reserved. |
+
    <div class="copyright1">Contact Us : oucigem@163.com  | &copy;2018 OUC IGEM.All Rights Reserved. <br />
−
 
+
<img src="https://static.igem.org/mediawiki/2017/b/b4/T--OUC-China--foot1.jpeg"alt="banner"width="80px">
−
  ………… </div>
+
<img src="https://static.igem.org/mediawiki/2017/6/62/T--OUC-China--foot2.jpeg"alt="banner"width="80px">
 +
<img src="https://static.igem.org/mediawiki/2018/f/f3/T--OUC-China--lalala.png"alt="banner"width="80px">
 +
<img src="https://static.igem.org/mediawiki/2017/5/51/T--OUC-China--NSG.png"alt="banner"height="65px">
 +
<img src="https://static.igem.org/mediawiki/2017/2/2a/T--OUC-China--ML.png"alt="banner"height="65px">&emsp;
 +
  </div>
 
 
  

Latest revision as of 17:19, 17 October 2018

Team OUC-China: Main
banner

Safety

Safety is the acutest problem when it comes to genetically modified organism. We figured this problem out from three aspects: Project design, Lab work and Shipment.

Project design


Being responsible for our earth and ourselves is our attitude towards every experiment. In our project, all the parts we have utilized are selected from Risk Group 1, none of which are with a Red Flag. What’s more, nonpathogenic bacteria, E.coli DH5 Alpha is employed as the main chassis for our prior design on the DNA sequence, stem-loop and enzyme. As a matter of fact, when using hairpins and csy4 enzyme to tune the expression of genes, it is relatively safe for industrialized applications and scientific research. Based on this, We designed more variants of csy4 enzymes and hairpins. （Click here to see more! ）So we used these to create a toolkit named miniToe family for synthetic biology which can control the expression of the target gene gradient. What’s more, we used the motA gene, one of the genes regulating the motility of E.coli, to verify the feasibility and effectiveness of the toolkit. For the sake of environment, all the chassis organisms will be sterilized before being abandoned to prevent the genes from leaking.

Safe lab work


We works at a neat lab named “Lab for Microinnovation and Enterprise”, which is a BSL-2 laboratory. Before working in the lab, we are supposed to be trained by the skillful hands, not only for the general lab safety rules, but also for the standard experimental operation avoiding unnecessary damage. No experiment carries no risk to the experimenters, hence we are properly equipped with facilities, such as latex gloves, nitrile gloves, goggles, lab coats to protect us from biotic and abiotic hazards.


Fig.1 Our laboratory and personal safety precautions

Then for lab safety, a routine inspection is performed twice a day by everyone, not only for ensuring normal operation of our lab like supplying experimental consumables and inspecting the running of instruments, but also keeps it away from non-biological hazards, especially the water and electricity. For high efficiency, responsibility implements to every team member.


Fig.2 Table for routine inspection

Furthermore, several operational zones are divided scientifically for special experiments like A zone where is weighing the drugs, preparing the medium and sterilizing, B zone where is the recording experimental note and organizing experimental data area, C zone where is a molecular operation area, D zone where is RNA electrophoresis and a polyacrylamide gel electrophoresis area. For us, lab safety is a hot topic and we have come up with a practical lab safety manual which normalizes our lovely lab.

Practical lab safety manual


1.Routine inspections must perform between 6:30-8:00pm and 9:30-11:00pm.
2.Lab cleaning must be done twice a week with disinfectant moping the floor for at least 3times.
3.Well prepared experiment plans should be written before you start.
4.Lab coats and gloves must be worn when conducting experiments.
5.Short skirts, shorts, and open shoes must not be worn.
6.If you see a colleague doing something dangerous, point it out to him or her.
7.Lab coats must not be worn outside laboratories and in public areas.
8.Know where safety equipment (eyewash, shower and extinguisher) is located.
9.Food/drink is not allowed in laboratories where chemicals are used stored.
10.Always read the directions before using instruments and read MSDS before handling new chemicals carefully.



Fig.3 Safety operation

Safe shipment


For submitting our parts to igem HQ, we first took the initiative to consult Chinese entry-exit inspection and quarantine laws and regulations about shipping DNA from China to the USA, which is forbidden without tedious red tape. Fortunately, GenScript cooperated with igem HQ forward our part sample to the USA. Ergo, we sealed dried DNA and sent it in the submission kit to GenScript, which clearly labelled as DNA without disguise. Our kit poses no risks and is perfectly safe for shipment.

Safety form


Click here to check our safety form.





Contact Us : oucigem@163.com | ©2018 OUC IGEM.All Rights Reserved.
banner banner banner banner banner