Difference between revisions of "Team:ICT-Mumbai/Parts"

 
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<h1>Parts</h1>
 
<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<p>The aim of our project is to isolate the promoters of the genes that are upregulated in presence of root exudates of the crops and use these promoters in different applications. As a case study, we worked on construction of a positive feedback circuit to amplify the production of the enzyme alkaline phosphatase in response to root exudates, during which we prepared several composite parts and intermediates and submitted them to the Registry.</p>
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<h4>Description</h4>
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  <li><h5><a href="http://parts.igem.org/Part:BBa_K2802000">BBa_K2802000:</a> </h5></li>
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<p>This part is a composite part composed of BBa_K274380 and BBa_I746351. A promoter can be cloned upstream of this part, and the PSP3 <I>pag</I> phage activator that will be produced will bind to the P<sub>F</sub> phage promoter to drive expression of the PSP3 <I>pag</I> phage activator, forming a genetic amplification circuit. </p>
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  <li><h5><a href="http://parts.igem.org/Part:BBa_K2802001">BBa_K2802001:</a> </h5></li>
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<p>This part is a composite part composed of BBa_K274380, BBa_I746351 and BBa_K518012. A promoter can be cloned upstream of this part, and the PSP3 <I>pag</I> phage activator that will be produced will bind to the P<sub>F</sub> phage promoter to drive expression of the PSP3 <I>pag</I> phage activator, forming a genetic amplification circuit. RFP, which will be produced from the gene cloned downstream of the second <I>pag</I> gene, will act as a detectable output of this genetic amplification circuit.
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  <li><h5><a href="http://parts.igem.org/Part:BBa_K2802002">BBa_K2802002:</a> </h5></li>
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<p>This part is a composite part composed of BBa_K274371 and BBa_I746350. A promoter can be cloned upstream of this part, and the P2 <I>ogr</I> phage activator that will be produced will bind to the P<sub>O</sub> phage promoter to drive expression of the P2 <I>ogr</I> phage activator, forming a genetic amplification circuit.</p>
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  <li><h5><a href="http://parts.igem.org/Part:BBa_K2802003">BBa_K2802003:</a> </h5></li>
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<p>This part is a composite part composed of BBa_K274371, BBa_I746350 and BBa_K518012. A promoter can be cloned upstream of this part, and the P2 <I>ogr</I> phage activator that will be produced will bind to the P<sub>O</sub> phage promoter to drive expression of the P2 <I>ogr</I> phage activator, forming a genetic amplification circuit. RFP, which will be produced from the gene cloned downstream of the second <I>ogr</I> gene, will act as a detectable output of this genetic amplification circuit.</p>
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<h4>Composite Parts Table</h4>
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<center><groupparts>iGEM18 ICT-Mumbai</groupparts></center>
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<h3>Adding parts to the registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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ADD PARTS
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<h4>Intermediate Parts Table</h4>
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<th>Name</th>
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<th>Type</th>
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<th>Description</th>
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<th>Designer</th>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_S05458">BBa_S05458</a></td>
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<td>Intermediate</td>
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<td>K274380:I746351 followed by <i>Bacillus subtilis</i> RBS-phoD gene</td>
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<td>Shamlan M. S. Reshamwala</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_S05459">BBa_S05459</a></td>
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<td>Intermediate</td>
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<td>K274371:I746350 followed by <i>Bacillus subtilis</i> RBS-phoD gene</td>
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<h3>Inspiration</h3>
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<td>Shamlan M. S. Reshamwala</td>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h3>What information do I need to start putting my parts on the Registry?</h3>
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<p>The information needed to initially create a part on the Registry is:</p>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h3>Part Table </h3>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM18 ICT-Mumbai</groupparts>
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Latest revision as of 17:33, 17 October 2018

Simply




Contact us: igemteamictmumbai@gmail.com  | Follow us:

Parts

The aim of our project is to isolate the promoters of the genes that are upregulated in presence of root exudates of the crops and use these promoters in different applications. As a case study, we worked on construction of a positive feedback circuit to amplify the production of the enzyme alkaline phosphatase in response to root exudates, during which we prepared several composite parts and intermediates and submitted them to the Registry.


Description

  1. BBa_K2802000:

    2. This part is a composite part composed of BBa_K274380 and BBa_I746351. A promoter can be cloned upstream of this part, and the PSP3 pag phage activator that will be produced will bind to the PF phage promoter to drive expression of the PSP3 pag phage activator, forming a genetic amplification circuit.

  2. BBa_K2802001:

    3. This part is a composite part composed of BBa_K274380, BBa_I746351 and BBa_K518012. A promoter can be cloned upstream of this part, and the PSP3 pag phage activator that will be produced will bind to the PF phage promoter to drive expression of the PSP3 pag phage activator, forming a genetic amplification circuit. RFP, which will be produced from the gene cloned downstream of the second pag gene, will act as a detectable output of this genetic amplification circuit.

  3. BBa_K2802002:

    4. This part is a composite part composed of BBa_K274371 and BBa_I746350. A promoter can be cloned upstream of this part, and the P2 ogr phage activator that will be produced will bind to the PO phage promoter to drive expression of the P2 ogr phage activator, forming a genetic amplification circuit.

  4. BBa_K2802003:

    5. This part is a composite part composed of BBa_K274371, BBa_I746350 and BBa_K518012. A promoter can be cloned upstream of this part, and the P2 ogr phage activator that will be produced will bind to the PO phage promoter to drive expression of the P2 ogr phage activator, forming a genetic amplification circuit. RFP, which will be produced from the gene cloned downstream of the second ogr gene, will act as a detectable output of this genetic amplification circuit.

Composite Parts Table

<groupparts>iGEM18 ICT-Mumbai</groupparts>


Intermediate Parts Table

Name Type Description Designer
BBa_S05458 Intermediate K274380:I746351 followed by Bacillus subtilis RBS-phoD gene Shamlan M. S. Reshamwala
BBa_S05459 Intermediate K274371:I746350 followed by Bacillus subtilis RBS-phoD gene Shamlan M. S. Reshamwala