Difference between revisions of "Team:ICT-Mumbai/Parts"

 
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<h1>Parts</h1>
 
<h1>Parts</h1>
 
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<p>Aim of our project is to isolate the promoters of the genes that are upregulated in presence of root exudates of the crops and use these promoters in different applications. As a case study, we worked on construction of a positive feedback circuit to amplify the production of PhoD (alkaline phosphatase) in response to root exudates, during which we prepared several composite parts and intermediates and submitted them to registry.</p>
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<p>The aim of our project is to isolate the promoters of the genes that are upregulated in presence of root exudates of the crops and use these promoters in different applications. As a case study, we worked on construction of a positive feedback circuit to amplify the production of the enzyme alkaline phosphatase in response to root exudates, during which we prepared several composite parts and intermediates and submitted them to the Registry.</p>
 
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<h4>Description</h4>
 
<ol type="1">
 
<ol type="1">
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   <li>BBa_2802000:</li>
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   <li><h5><a href="http://parts.igem.org/Part:BBa_K2802000">BBa_K2802000:</a> </h5></li>
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<p>This part is a composite part composed of BBa_K274380 and BBa_I746351. A promoter can be cloned upstream of this part, and the PSP3 <I>pag</I> phage activator that will be produced will bind to the PF phage promoter to drive expression of the PSP3 <I>pag</I> phage activator, forming a genetic amplifier. </p>
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<p>This part is a composite part composed of BBa_K274380 and BBa_I746351. A promoter can be cloned upstream of this part, and the PSP3 <I>pag</I> phage activator that will be produced will bind to the P<sub>F</sub> phage promoter to drive expression of the PSP3 <I>pag</I> phage activator, forming a genetic amplification circuit. </p>
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   <li>BBa_2802001:</li>
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   <li><h5><a href="http://parts.igem.org/Part:BBa_K2802001">BBa_K2802001:</a> </h5></li>
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<p>This part is a composite part composed of BBa_K274380, BBa_I746351 and BBa_K518012. A promoter can be cloned upstream of this part, and the PSP3 <I>pag</I> phage activator that will be produced will bind to the PFF phage promoter to drive expression of the PSP3 <I>pag</I> phage activator, forming a genetic amplifier. <I>rfp</I>, which will be produced from the gene cloned downstream of the second <I>pag</I> gene, will act as a detectable output of this genetic amplifier.
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<p>This part is a composite part composed of BBa_K274380, BBa_I746351 and BBa_K518012. A promoter can be cloned upstream of this part, and the PSP3 <I>pag</I> phage activator that will be produced will bind to the P<sub>F</sub> phage promoter to drive expression of the PSP3 <I>pag</I> phage activator, forming a genetic amplification circuit. RFP, which will be produced from the gene cloned downstream of the second <I>pag</I> gene, will act as a detectable output of this genetic amplification circuit.
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   <li>BBa_2802002:</li>
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   <li><h5><a href="http://parts.igem.org/Part:BBa_K2802002">BBa_K2802002:</a> </h5></li>
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<p>This part is a composite part composed of BBa_K274371 and BBa_I746350. A promoter can be cloned upstream of this part, and the P2 <I>ogr</I> phage activator that will be produced will bind to the PO phage promoter to drive expression of the P2 <I>ogr</I> phage activator, forming a genetic amplifier.</p>
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<p>This part is a composite part composed of BBa_K274371 and BBa_I746350. A promoter can be cloned upstream of this part, and the P2 <I>ogr</I> phage activator that will be produced will bind to the P<sub>O</sub> phage promoter to drive expression of the P2 <I>ogr</I> phage activator, forming a genetic amplification circuit.</p>
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<li>BBa_2802003:</li>
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  <li><h5><a href="http://parts.igem.org/Part:BBa_K2802003">BBa_K2802003:</a> </h5></li>
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<p>This part is a composite part composed of BBa_K274371, BBa_I746350 and BBa_K518012. A promoter can be cloned upstream of this part, and the P2 <I>ogr</I> phage activator that will be produced will bind to the PO phage promoter to drive expression of the P2 <I>ogr</I> phage activator, forming a genetic amplifier. RFP, which will be produced from the gene cloned downstream of the second <I>ogr</I> gene, will act as a detectable output of this genetic amplifier.</p>
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<p>This part is a composite part composed of BBa_K274371, BBa_I746350 and BBa_K518012. A promoter can be cloned upstream of this part, and the P2 <I>ogr</I> phage activator that will be produced will bind to the P<sub>O</sub> phage promoter to drive expression of the P2 <I>ogr</I> phage activator, forming a genetic amplification circuit. RFP, which will be produced from the gene cloned downstream of the second <I>ogr</I> gene, will act as a detectable output of this genetic amplification circuit.</p>
 
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<h4>Composite Parts Table</h4>
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<center><groupparts>iGEM18 ICT-Mumbai</groupparts></center>
 
<center><groupparts>iGEM18 ICT-Mumbai</groupparts></center>
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<h4>Intermediate Parts Table</h4>
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Following are the intermediate parts submitted by the team to the iGEM registry</p>
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<table class="center" id="cssTable">
 
<table class="center" id="cssTable">
 
<tr>
 
<tr>
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<th>Description</th>
 
<th>Description</th>
 
<th>Designer</th>
 
<th>Designer</th>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_S05458">BBa_S05458</a></td>
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<td>Intermediate</td>
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<td>K274380:I746351 followed by <i>Bacillus subtilis</i> RBS-phoD gene</td>
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<td>Shamlan M. S. Reshamwala</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_S05459">BBa_S05459</a></td>
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<td>Intermediate</td>
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<td>K274371:I746350 followed by <i>Bacillus subtilis</i> RBS-phoD gene</td>
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<td>Shamlan M. S. Reshamwala</td>
 
</tr>
 
</tr>
 
</table>
 
</table>

Latest revision as of 17:33, 17 October 2018

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Parts

The aim of our project is to isolate the promoters of the genes that are upregulated in presence of root exudates of the crops and use these promoters in different applications. As a case study, we worked on construction of a positive feedback circuit to amplify the production of the enzyme alkaline phosphatase in response to root exudates, during which we prepared several composite parts and intermediates and submitted them to the Registry.


Description

  1. BBa_K2802000:

    2. This part is a composite part composed of BBa_K274380 and BBa_I746351. A promoter can be cloned upstream of this part, and the PSP3 pag phage activator that will be produced will bind to the PF phage promoter to drive expression of the PSP3 pag phage activator, forming a genetic amplification circuit.

  2. BBa_K2802001:

    3. This part is a composite part composed of BBa_K274380, BBa_I746351 and BBa_K518012. A promoter can be cloned upstream of this part, and the PSP3 pag phage activator that will be produced will bind to the PF phage promoter to drive expression of the PSP3 pag phage activator, forming a genetic amplification circuit. RFP, which will be produced from the gene cloned downstream of the second pag gene, will act as a detectable output of this genetic amplification circuit.

  3. BBa_K2802002:

    4. This part is a composite part composed of BBa_K274371 and BBa_I746350. A promoter can be cloned upstream of this part, and the P2 ogr phage activator that will be produced will bind to the PO phage promoter to drive expression of the P2 ogr phage activator, forming a genetic amplification circuit.

  4. BBa_K2802003:

    5. This part is a composite part composed of BBa_K274371, BBa_I746350 and BBa_K518012. A promoter can be cloned upstream of this part, and the P2 ogr phage activator that will be produced will bind to the PO phage promoter to drive expression of the P2 ogr phage activator, forming a genetic amplification circuit. RFP, which will be produced from the gene cloned downstream of the second ogr gene, will act as a detectable output of this genetic amplification circuit.

Composite Parts Table

<groupparts>iGEM18 ICT-Mumbai</groupparts>


Intermediate Parts Table

Name Type Description Designer
BBa_S05458 Intermediate K274380:I746351 followed by Bacillus subtilis RBS-phoD gene Shamlan M. S. Reshamwala
BBa_S05459 Intermediate K274371:I746350 followed by Bacillus subtilis RBS-phoD gene Shamlan M. S. Reshamwala