Difference between revisions of "Team:ECUST/Biocide"

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<h1 class="box-heading">Result</h1>
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<p>We insert fragment of Cecropin AD into vector pET-28a(Figure ) </p>
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<p>The plasmid was transformed to E. coli BL21 and cultured at 37 °C for 12 h. positive monoclonal bacteria were cultured and verified by PCR(Figure). </p>
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<p>We verified the expression of Cecropin AD by SDS-PAGE(Figure). </p>
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Revision as of 17:40, 17 October 2018

BIOCIDE

Description

When the concentration of Fe3+ in the environment is high, it means that the hard skeleton of the microenvironment of the iron bacteria is destroyed and loses protection. At this time, the added bactericide will have a greater killing effect on the bacteria. The bactericide we selected was cecropin AD, a cationic chimeric antimicrobial peptide obtained from cecropin, consisting of the first 11 amino acid residues of cecropin A and the last 26 amino acid residues of cecropin D. It has strong antibacterial activity against Gram-positive and negative bacteria.

Design