Difference between revisions of "Team:ICT-Mumbai/Composite Part"

 
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<h1>Parts</h1>
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<p>This year, Team ICT-Mumbai contributed four composite parts towards the iGEM Parts Registry. Our best composite part is described below:</p>
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<h4>Best Composite Part</h4>
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<h5>BBa_K2802001:</h5>
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<p>This part is a composite part composed of BBa_K274380, BBa_I746351 and BBa_K518012. A promoter can be cloned upstream of this part, and the PSP3 <I>pag</I> phage activator that will be produced will bind to the P<sub>F</sub> phage promoter to drive expression of the PSP3 <I>pag</I> phage activator, forming a genetic amplification circuit. RFP, which will be produced from the gene cloned downstream of the second <I>pag</I> gene, will act as a detectable output of this genetic amplification circuit.</p>
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<h5>Design</h5>
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BBa_K2802000 and BBa_K518012 were joined together using 3A Assembly.</p>
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<img style="display: block; margin: auto; height: 400px;" src="https://static.igem.org/mediawiki/2018/1/14/T--ICT-Mumbai--Best-composite.jpg">
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The 718 bp BBa_K2802000 (lane 2) and 828 bp BBa_K518012 parts were joined together to make 1554 bp BBa_K2802001 (lane 3). The plasmid backbone is pSB1C3. Plasmids were digested with EcoRI and PstI to release the parts and determine correct assembly of the parts.
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<h4>Other Composite Parts</h4>
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<li><h5>BBa_K2802000:</h5></li>
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<p>This part is a composite part composed of BBa_K274380 and BBa_I746351. A promoter can be cloned upstream of this part, and the PSP3 <I>pag</I> phage activator that will be produced will bind to the P<sub>F</sub> phage promoter to drive expression of the PSP3 <I>pag</I> phage activator, forming a genetic amplification circuit. </p>
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<li><h5>BBa_K2802002:</h5></li>
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<p>This part is a composite part composed of BBa_K274371 and BBa_I746350. A promoter can be cloned upstream of this part, and the P2 <I>ogr</I> phage activator that will be produced will bind to the P<sub>O</sub> phage promoter to drive expression of the P2 <I>ogr</I> phage activator, forming a genetic amplification circuit.</p>
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<li><h5>BBa_K2802003:</h5></li>
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<p>This part is a composite part composed of BBa_K274371, BBa_I746350 and BBa_K518012. A promoter can be cloned upstream of this part, and the P2 <I>ogr</I> phage activator that will be produced will bind to the P<sub>O</sub> phage promoter to drive expression of the P2 <I>ogr</I> phage activator, forming a genetic amplification circuit. RFP, which will be produced from the gene cloned downstream of the second <I>ogr</I> gene, will act as a detectable output of this genetic amplification circuit.</p>
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<h4>Composite Parts Table</h4>
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<center><groupparts>iGEM18 ICT-Mumbai</groupparts></center>
  
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Composite Parts</h1>
 
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
 
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
 
 
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<h3>Note</h3>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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<h3>Best Composite Part Special Prize</h3>
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<p>To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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Latest revision as of 17:44, 17 October 2018

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Parts

This year, Team ICT-Mumbai contributed four composite parts towards the iGEM Parts Registry. Our best composite part is described below:

Best Composite Part

BBa_K2802001:

This part is a composite part composed of BBa_K274380, BBa_I746351 and BBa_K518012. A promoter can be cloned upstream of this part, and the PSP3 pag phage activator that will be produced will bind to the PF phage promoter to drive expression of the PSP3 pag phage activator, forming a genetic amplification circuit. RFP, which will be produced from the gene cloned downstream of the second pag gene, will act as a detectable output of this genetic amplification circuit.

Design

BBa_K2802000 and BBa_K518012 were joined together using 3A Assembly.


The 718 bp BBa_K2802000 (lane 2) and 828 bp BBa_K518012 parts were joined together to make 1554 bp BBa_K2802001 (lane 3). The plasmid backbone is pSB1C3. Plasmids were digested with EcoRI and PstI to release the parts and determine correct assembly of the parts.

Other Composite Parts

  1. BBa_K2802000:

    2. This part is a composite part composed of BBa_K274380 and BBa_I746351. A promoter can be cloned upstream of this part, and the PSP3 pag phage activator that will be produced will bind to the PF phage promoter to drive expression of the PSP3 pag phage activator, forming a genetic amplification circuit.

  2. BBa_K2802002:

    3. This part is a composite part composed of BBa_K274371 and BBa_I746350. A promoter can be cloned upstream of this part, and the P2 ogr phage activator that will be produced will bind to the PO phage promoter to drive expression of the P2 ogr phage activator, forming a genetic amplification circuit.

  3. BBa_K2802003:

    4. This part is a composite part composed of BBa_K274371, BBa_I746350 and BBa_K518012. A promoter can be cloned upstream of this part, and the P2 ogr phage activator that will be produced will bind to the PO phage promoter to drive expression of the P2 ogr phage activator, forming a genetic amplification circuit. RFP, which will be produced from the gene cloned downstream of the second ogr gene, will act as a detectable output of this genetic amplification circuit.

Composite Parts Table

<groupparts>iGEM18 ICT-Mumbai</groupparts>