Difference between revisions of "Team:NUS Singapore-Sci/enzyme kinetics"

(Created page with "{{NUS_Singapore-Sci}} <html> <head> <title> NUS Singapore Science: InterLab </title> <meta charset="UTF-8"> </head> <body> <h1 id="title"> <div style="font-size: 2.2em...")
 
Line 14: Line 14:
  
 
<div class="clear extra_space"></div>
 
<div class="clear extra_space"></div>
 +
 +
<div class="numberedsection">
 +
Enzyme Kinetics and Least Squares Regression
 +
</div>
 +
 +
<div class="text">
 +
The efficiency of our RESCUE system is likely to be dependent on multiple factors such as mismatch distance, length of spacer regions as with ADAR-dCas13b constructs (Cox <i>et al.</i>, 2016), as well as the relative concentrations of the substrates/enzymes. The concept of regression models can be utilized to identify and evaluate the significance of these factors from experimental results. As such, a early build of an enzyme kinetics regression model on dCas13b-APOBEC editing efficiency may help us to gain further insights of the RESCUE system.
 +
</div>
 +
 +
<div class="section">
 +
Goal
 +
</div>

Revision as of 18:00, 17 October 2018

NUS Singapore Science: InterLab

Enzyme
Kinetics

Enzyme Kinetics and Least Squares Regression
The efficiency of our RESCUE system is likely to be dependent on multiple factors such as mismatch distance, length of spacer regions as with ADAR-dCas13b constructs (Cox et al., 2016), as well as the relative concentrations of the substrates/enzymes. The concept of regression models can be utilized to identify and evaluate the significance of these factors from experimental results. As such, a early build of an enzyme kinetics regression model on dCas13b-APOBEC editing efficiency may help us to gain further insights of the RESCUE system.
Goal