Difference between revisions of "Team:Austin LASA/Description"

Revision as of 20:19, 17 October 2018

const r = i => h('a', {href: '#ref_' + i}, i);
h(g.Page, {title: 'Description', prev: 'https://2018.igem.org/Team:Austin_LASA/Collaborations', next: 'https://2018.igem.org/Team:Austin_LASA/Design', selector: [2, 0]},
  h(g.Section, {title: 'Why HIV Detection in Infants?'},
    h('p', null, 'HIV diagnosis of infants in the developing world continues to pose many problems. It is estimated that there are approximately 1500 new cases of HIV infection in children under the age of 15, 90% of which occur in the developing world and contracted HIV at birth [', r(1), ']. Infants often show symptoms of HIV very early on, and in some cases, HIV can rapidly evolve into AIDS [', r(2), ']. As such, it is imperative that infants with HIV are diagnosed as early as possible.'),
    h('p', null, 'The WHO has stated that earlier diagnosis of HIV in infants and children:'),
    h('ul', null,
      h('li', null, 'enables the early identification of who have HIV-infection, as a first step in securing their treatment and care'),
      h('li', null, 'enables the identification of those who are HIV-exposed but uninfected, facilitating follow-up care and prevention measures that will help to ensure that they remain uninfected'),
      h('li', null, 'assists in the effective use of essential resources by targeting ART on children who need treatment'),
      h('li', null, 'improves the psychosocial well-being of families and children, reducing potential stigma, discrimination and psychological distress for HIV-uninfected children and increasing the chances of adoption for orphans'),
      h('li', null, 'facilitates life-planning for parents and/or children who have HIV [', r(3), ']')
    ),
    h('p', null, 'Unfortunately, HIV diagnosis in infants faces more challenges than HIV diagnosis in adults. Typical serological assays result in large false positives and negatives as infants retain maternal HIV antibodies for up to 18 months, initially obtaining them through transplacental fluid in utero or through breastfeeding milk postnatally, and are therefore unreliable for use as a diagnostic method [', r(3), ']. As a result, HIV DNA/RNA PCR tests, p24 core antigen tests, and HIV cultures are the only reliable diagnostic tests for HIV in infants [', r(3), ', ', r(4), ']. PCR tests, although reliable, are frequently unavailable in resource-constrained settings where the proper lab equipment and skilled personnel are limited [', r(5), ']. HIV cultures, similarly to PCR tests, require proper lab equipment and skilled personnel in addition to taking up to two weeks to grow and obtain results [, r(3), ']. p24 core antigen tests also require proper lab equipment and skilled personnel, but are undergoing testing and clinical evaluation for their utility [', r(5), '].')
  ),
  h(g.Section, {title: 'References'},
    h('p', null, '[', h('a', {id: 'ref_1'}, '1'), '] W. (2011, February 03). Antiretroviral therapy of HIV infection in infants and children. Retrieved October 16, 2018, from ', h('a', {href: 'http://www.who.int/hiv/pub/paediatric/infants/en/'}, 'http://www.who.int/hiv/pub/paediatric/infants/en/')),
    h('p', null, '[', h('a', {id: 'ref_2'}, '2'), '] Krist, A. H., & Crawford-Faucher, A. (2002, May 15). Management of Newborns Exposed to Maternal HIV Infection. Retrieved October 16, 2018, from ', h('a', {href: 'https://www.aafp.org/afp/2002/0515/p2049.html'}, 'https://www.aafp.org/afp/2002/0515/p2049.html')),
    h('p', null, '[', h('a', {id: 'ref_3'}, '3'), '] Damet, G., & Vercauteren, G. (n.d.). Early detection of HIV infection in infants and children [PDF]. Geneva: WHO.'),
    h('p', null, '[', h('a', {id: 'ref_4'}, '4'), '] Diagnosis of HIV Infection in Infants and Children Pediatric ARV. (2017, November 15). Retrieved October 16, 2018, from ', h('a', {href: 'https://aidsinfo.nih.gov/guidelines/html/2/pediatric-arv/55/diagnosis-of-hiv-infection-in-infants-and-children'}, 'https://aidsinfo.nih.gov/guidelines/html/2/pediatric-arv/55/diagnosis-of-hiv-infection-in-infants-and-children')),
    h('p', null, '[', h('a', {id: 'ref_5'}, '5'), '] Shafiee, H., Wang, S., Inci, F., Toy, M., Henrich, T. J., Kuritzkes, D. R., & Demirci, U. (2015). Emerging technologies for point-of-care management of HIV infection. Annual review of medicine, 66, 387-405.'),
    h('p', null, '[', h('a', {id: 'ref_6'}, '6'), '] Gootenberg, J. S., Abudayyeh, O. O., Kellner, M. J., Joung, J., Collins, J. J., & Zhang, F. (2018). Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science, 360(6387), 439-444.'),
    h('p', null, '[', h('a', {id: 'ref_7'}, '7'), '] Chen, J. S., Ma, E., Harrington, L. B., Da Costa, M., Tian, X., Palefsky, J. M., & Doudna, J. A. (2018). CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Science, 360(6387), 436-439.')
  )
);

@@ Line 14: / Line 14: @@
        h('li', null, 'facilitates life-planning for parents and/or children who have HIV [', r(3), ']')
      ),
-     h('p', null, 'Unfortunately, HIV diagnosis in infants faces more challenges than HIV diagnosis in adults. Typical serological assays result in large false positives and negatives as infants retain maternal HIV antibodies for up to 18 months, initially obtaining them through transplacental fluid in utero or through breastfeeding milk postnatally, and are therefore unreliable for use as a diagnostic method [', r(3), ']. As a result, HIV DNA/RNA PCR tests, p24 core antigen tests, and HIV cultures are the only reliable diagnostic tests for HIV in infants [', r(3), ', ', r(4), ']. PCR tests, although reliable, are frequently unavailable in resource-constrained settings where the proper lab equipment and skilled personnel are limited [', r(5), ']. HIV cultures, similarly to PCR tests, require proper lab equipment and skilled personnel in addition to taking up to two weeks to grow and obtain results [, r(3), ']. p24 core antigen tests also require proper lab equipment and skilled personnel, but are undergoing testing and clinical evaluation for their utility [, r(5), '].')
+     h('p', null, 'Unfortunately, HIV diagnosis in infants faces more challenges than HIV diagnosis in adults. Typical serological assays result in large false positives and negatives as infants retain maternal HIV antibodies for up to 18 months, initially obtaining them through transplacental fluid in utero or through breastfeeding milk postnatally, and are therefore unreliable for use as a diagnostic method [', r(3), ']. As a result, HIV DNA/RNA PCR tests, p24 core antigen tests, and HIV cultures are the only reliable diagnostic tests for HIV in infants [', r(3), ', ', r(4), ']. PCR tests, although reliable, are frequently unavailable in resource-constrained settings where the proper lab equipment and skilled personnel are limited [', r(5), ']. HIV cultures, similarly to PCR tests, require proper lab equipment and skilled personnel in addition to taking up to two weeks to grow and obtain results [, r(3), ']. p24 core antigen tests also require proper lab equipment and skilled personnel, but are undergoing testing and clinical evaluation for their utility [', r(5), '].')
    ),
    h(g.Section, {title: 'References'},