Line 24: | Line 24: | ||
<p> The Glycolate oxidase we submitted was cloned from /E. coli/ MG1655 and contains three subunits. It was inserted into the pETDuet vector for expression and was inducted by 0.1and 0.2 mM IPTG at 30ºC for 20 hours. | <p> The Glycolate oxidase we submitted was cloned from /E. coli/ MG1655 and contains three subunits. It was inserted into the pETDuet vector for expression and was inducted by 0.1and 0.2 mM IPTG at 30ºC for 20 hours. | ||
− | All three subunit of Glycolate Oxidase(GO) are shown in the SDS-PAGE. GO contains three subunits, D,E, and F, which weigh 55.5 kDa, 38.3 kDa, and 45.0 kDa respectively. Lane 1 was BL21(DE3) with pETDuet-GO, but without IPTG induction process. Lanes 2 and 3 were BL21(DE3) with empty pETDute vectors and contain 0.1 and 0.2 mM IPTG. Lanes 4 to 7 are BL21(De3) with pETDuet-GO. Lanes 4 and 6 were | + | All three subunit of Glycolate Oxidase(GO) are shown in the SDS-PAGE. GO contains three subunits, D,E, and F, which weigh 55.5 kDa, 38.3 kDa, and 45.0 kDa respectively. Lane 1 was BL21(DE3) with pETDuet-GO, but without IPTG induction process. Lanes 2 and 3 were BL21(DE3) with empty pETDute vectors and contain 0.1 and 0.2 mM IPTG. Lanes 4 to 7 are BL21(De3) with pETDuet-GO. Lanes 4 and 6 were induced with 0.1mM IPTG, and lanes 5 and 7 were induced with 0.2mM IPTG. All the inductions were done at 30ºC. |
</p> | </p> | ||
<center> | <center> |
Revision as of 20:42, 17 October 2018