The William and Mary iGEM team is deeply grateful to all who supported the team throughout the year. Behind our team is a community of supporters, whether advisors, friends, or sponsors, and our project would not have been possible without them.

We would like to thank Dr. Margaret Saha for the instrumental role she played in our project. As our PI and team advisor, she has poured a great amount of energy into ensuring our team has the tools we need to succeed. Her dedication and boundless enthusiasm have been invaluable and for this we are forever grateful. We would also like to thank Dr. Patel for his guidance with our math modeling; he has gone above and beyond in mentoring the math team.

We’d like to thank Dr. Eric Bradley for his assistance with lab facilities, Dr. Mark Forsyth, and Dr. Joshua Puzey. We’d also like to thank Jacob Sawyer, advanced imaging specialist, for his microscopy guidance.

We would also like to thank John Marken our student advisor for his assistance in developing the conceptual framework of our project, as well as for his mentoring of our math team and for his invaluable advice on the presentation of our project.

Special thanks to Andy Halleran of Richard Murray Lab at the California Institute of Technology for his technical support and for providing us with a starter set of 3G compatible parts as well as protocols and advice on using them.

Our outreach efforts would not have been possible without support from the following:

• Anne Petersen: Science Coordinator for the Virginia Department of Education
• Jennifer Daugherty: Regional Sales Manager for Thermofisher Scientific
• Donna Farnham: Counsel Event Assistant for Girl Scouts of the Colonial Coast.
• Jacqueline Brooks: Counsel Partnership and Event Manager Girl Scouts of the Colonial Coast
• Ashley Zhang: Under Secretary General of the General Assembly William and Mary Model United Nations
• Laura Mullen: Manager of Activitie,s Williamsburg Landing
• John Leone: Williamsburg-James City Public School Educator
• Deanna Marroletti: Camp Launch
• Surgeon Singh: Spira, CTO (business speaker)
• Amanda Synder: Medical Explorers
• Patel Ramsey: WindsorMeade, Lifestyle Coordinator
• Valerie Cushman: Women’s Weekend, Director of Alumni Initiatives William and Mary
• Graham Henshaw: Clinical professor at the William and Mary Mason School of Business, Executive Director of the Entrepreneurship Center
• NASA: Mini-proposal (funding)
• William and Mary STEMinist Club
• Williamsburg Classical Academy (homeschooling coalition)
• Building with Biology
• Williamsburg Gazette
• Melissa Degaraff - Administrative Coordinator Christopher Wren Association

Dr. Dennis Manos has been an invaluable resource in providing financial support. We would also like to thank the following organizations for generously providing supplies and funding to help our ideas become a reality:

• Epoch Life Sciences Inc
• BioBasic
• NEB

All work was performed by the undergraduate members of unless indicated otherwise. Our three high school trainees (Nitin Arora, Nikhil Arora and Davis Hatcher) helped us with some of our molecular cloning work in July (Colony PCRs, Gel electrophoresis) as a hands on experience to help them get acquainted with molecular cloning techniques while they designed their projects. All text, figures code, and experiments were conceived, designed and implemented by the team, with advising from our PIs and our student advisors.

D Ewen Cameron and James J Collins. Tunable protein degradation in bacteria. Nature biotechnology, 32(12):1276–1281, 20

Gal-Mor O, Valdez Y, Finlay BB. The temperature-sensing protein TlpA is repressed by PhoP and dispensable for virulence of Salmonella enterica serovar Typhimurium in mice. Microbes Infect / Institut Pasteur. 2006;8:2154–62. doi:10.1016/j.micinf.2006.04.015.

CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. Sonya V. Iverson, Traci L. Haddock, Jacob Beal, and Douglas M. Densmore. ACS Synthetic Biology 2016 5 (1), 99-103 DOI: 10.1021/acssynbio.5b00124

Kracikova, M., Akiri, G., George, A., Sachidanandam, R., and Aaronson, S.A. (2013). A threshold mechanism mediates p53 cell fate decision between growth arrest and apoptosis. Cell Death Differ. 20, 576–588.

Nandan Kumar Jana, Siddhartha Roy, Bhabatarak Bhattacharyya, Nitai Chandra Mandal; Amino acid changes in the repressor of bacteriophage lambda due to temperature-sensitive mutations in its cI gene and the structure of a highly temperature-sensitive mutant repressor, Protein Engineering, Design and Selection, Volume 12, Issue 3, 1 March 1999, Pages 225–233, https://doi.org/10.1093/protein/12.3.225

One-Step Cloning and Chromosomal Integration of DNA François St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin. ACS Synthetic Biology 2013 2 (9), 537-541. DOI: 10.1021/sb400021

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Zhang C, Tsoi R, Wu F, You L (2016) Processing Oscillatory Signals by Incoherent Feedforward Loops. PLoS Comput Biol 12(9): e1005101. doi:10.1371/journal.pcbi.1005101